A new isoform of interleukin-32 suppresses IL-8 mrna expression in the intestinal epithelial cell line ht-29

Imaeda, Hirotsugu; Andoh, Akira; Aomatsu, Tomoki; Osaki, Rie; Bamba, Shigeki; Inatomi, Osamu; Shimizu, Tomoharu; Fujiyama, Yoshihide

doi:10.3892/mmr.2011.442

Cited by 10 publications

(9 citation statements)

References 24 publications

(42 reference statements)

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“…The pro-inflammatory nature of IL-32 made it of great interest in the context of autoimmune diseases. Though most of the studies have been done in inflammatory bowel disease 34,35 and RA, IL-32 is also involved in a range of autoimmune disorders, including T1D, 12 ankylosing spondylitis 36 and granulomatosis with polyangiitis. 37 Furthermore, IL-32 is strongly expressed in peripheral blood mononuclear cells (PBMC) and in serum from patients with Grave's diseases.…”

Section: Il -32 In Autoimmunit Ymentioning

confidence: 99%

The role of Interleukin‐32 in autoimmunity

et al. 2021

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Interleukin 32 (IL-32) was first identified in 1992 as a novel human gene encoding a 27-KD protein expressed in activated natural killer (NK) cells and T cells in humans and was named NK4. 1 In 2005, Kim et al found that the product of NK4 possesses the characteristics of a pro-inflammatory cytokine, and accordingly, the name was changed to IL-32. 2 A growing body of evidence implicates IL-32 in health and disease. Its roles in host responses to pathogens 3,4 allergy and asthma, 5 cancer 6 and cardiovascular diseases 7 have been reviewed recently. The objective of this review is to discuss the role of IL-32 in autoimmune diseases and, in particular, rheumatoid arthritis (RA) and type 1 diabetes (T1D). 2 | GENE AND ISOFORMS The IL32 gene has at least eight exons that reside on human chromosome 16p13.3. 2 Interestingly, the gene is encoded in higher mammals but not in rodents. 8 As of May 2020, the Ensembl database (ensembl.org) contains 35 splice variant transcripts of the gene, of which 30 potentially encode proteins. The longest IL-32γ isoform is identical to the original NK4 transcript, and alternative splicing yields nine experimentally validated isoforms: IL-32α, IL-32β, IL-32γ, IL-32δ, IL-32ε, IL-32ζ, IL-32η, IL-32 and IL-32sm. 2,8-10 The moststudied isoforms are IL-32α, IL-32β, IL-32γ and IL-32δ, with gamma being the longest and the most-studied isoform. IL-32α, IL-32β and IL-32γ are structurally similar to each other, and IL-32η and IL-32θ are similar to each other. The

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Section: Il -32 In Autoimmunit Ymentioning

confidence: 99%

The role of Interleukin‐32 in autoimmunity

et al. 2021

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“…A new isoform of IL-32 has been identified in human colonic subepithelial myofibroblasts (SEMFs); this isoform lacks exon 3 and 4 of the IL-32γ isoform (longest isoform) and was named as IL-32ε [ 50 ]. An enhanced level of the transcript of IL-32ε was found in inflamed mucosa of IBD patients.…”

Section: Reviewmentioning

confidence: 99%

“…An enhanced level of the transcript of IL-32ε was found in inflamed mucosa of IBD patients. TNF-α, in a time- and dose-dependent manner, was found to be an active inducer of transcript of this new IL-32ε [ 50 ]. It has been reported previously in a study in HT-29 cells, that transfection of IL-32ε results in an overall decrease of IL-8 transcript mediated by TNFα, but the expression of the shortest isoform IL-32α, which lacks exon 3 and 7, showed no effect on the IL-8 transcript.…”

Section: Reviewmentioning

confidence: 99%

A panoramic spectrum of complex interplay between the immune system and IL-32 during pathogenesis of various systemic infections and inflammation

2015

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Cytokines have always been of great interest due to their vast potential and participation in the progression and pathogenesis of various ailments. Interleukin-32 (IL-32) is a recently identified cytokine, whose gene is located on human chromosome 16 p13.3, with eight exons and six splice variants (IL-32α to IL-32ζ). IL-32α, the most abundant form, is secreted by different types of cells including T cells, natural killer (NK) cells, monocytes, endothelial cells and epithelial cells. It acts as a preferential mediator and effector of abnormal immune responses to multiple inflammatory and auto immune diseases including rheumatoid arthritis, chronic obstructive pulmonary disease (COPD), inflammatory bowel disease (IBD), etc. It was found to stimulate the induction of various chemokines, pro-inflammatory cytokines including IL-1β, IL-6, IL-8, TNF-α and macrophage inflammatory protein-2 (MIP-2). Hence, IL-32 mediates the crucial interplay among immune system and body cells during pathogenesis of various insults. The aim of the present effort is to summarize the role, mechanism of pathogenesis and potential therapeutic applications of IL-32 in different systemic infections and diseased conditions.

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“…This leads to increased production of IL-6 and IL-1β ( 30 31 ). IL-32ε, an isoform of IL-32, has been identified in human colonic subepithelial myofibroblasts and found to be enhanced in the inflamed mucosa of patients with inflammatory bowel disease ( 32 ). The role of IL-32 in bowel inflammation has been investigated in vivo using IL-32γ TG mice ( 3 33 ), in which an increased concentration of IL-32γ was associated with faster and more severe progression of acute bowel inflammation compared to wild-type (WT) mice.…”

Section: Role Of Il-32 In Inflammatory Diseasesmentioning

confidence: 99%

Role of IL-32 Gamma on Bone Metabolism in Autoimmune Arthritis

et al. 2018

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IL-32 acts as a pro-inflammatory cytokine by inducing the synthesis of inflammatory molecules as well as promoting the morphological changes involved in the transformation of monocytes into osteoclasts (OCs). Evaluation of the functions of IL-32 has mainly focused on its inflammatory properties, such as involvement in the pathogenesis of various autoimmune diseases. Recently, IL-32 was shown to be involved in bone metabolism, in which it promotes the differentiation and activation of OCs and plays a key role in bone resorption in inflammatory conditions. IL-32γ also regulates bone formation in conditions such as ankylosing spondylitis and osteoporosis. In this review, we summarize the results of recent studies on the role of IL-32γ in bone metabolism in inflammatory arthritis.

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A new isoform of interleukin-32 suppresses IL-8 mrna expression in the intestinal epithelial cell line ht-29

Cited by 10 publications

References 24 publications

The role of Interleukin‐32 in autoimmunity

The role of Interleukin‐32 in autoimmunity

A panoramic spectrum of complex interplay between the immune system and IL-32 during pathogenesis of various systemic infections and inflammation

Role of IL-32 Gamma on Bone Metabolism in Autoimmune Arthritis

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