A New Integrative Conjugative Element Occurs in
            <i>Mycoplasma agalactiae</i>
            as Chromosomal and Free Circular Forms

Marenda, Marc S.; Barbe, Valérie; Gourgues, Géraldine; Mangenot, Sophie; Sagné, Evelyne; Citti, Christine

doi:10.1128/jb.00114-06

Cited by 45 publications

(83 citation statements)

References 23 publications

(18 reference statements)

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“…and Spiroplasma spp., have been shown to conjugatively transfer DNA (25,73,182,183,225,258,272). Mycoplasma pulmonis and Spiroplasma citri can transfer chromosomal DNA markers, and some early evidence suggested FIG.…”

Section: Cell Wall-less Systemsmentioning

confidence: 99%

“…73,2009 BIOLOGICAL DIVERSITY OF PROKARYOTIC T4SS 797 that bidirectional chromosomal transfer can occur during conjugation (25,182 (20,73,183,231,247). Interestingly, some M. fermentans ICEs lack homologs of known integrases, transposases, or recombinases, suggesting that a novel enzyme might be involved in the excision of these elements (43).…”

Section: Cell Wall-less Systemsmentioning

confidence: 99%

“…These proteins possess a repeated amino acid domain, localize extracellularly, and might mediate attachment to host target cells (see below). Other commonly associated genes code for ParA or Soj proteins or, intriguingly, in the case of S. kunkelii, MreB, a cell shape-determining protein postulated to be important for the helical morphology of spiroplasmas (20,183,231). These cytoskeletal proteins might function as spatial determinants for the assembly of the T4SS, reminiscent of the ParA-like proteins of gram-negative systems.…”

Section: Cell Wall-less Systemsmentioning

confidence: 99%

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Biological Diversity of Prokaryotic Type IV Secretion Systems

Martı́nez

Christie

2009

Microbiol Mol Biol Rev

621

780

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SUMMARY Type IV secretion systems (T4SS) translocate DNA and protein substrates across prokaryotic cell envelopes generally by a mechanism requiring direct contact with a target cell. Three types of T4SS have been described: (i) conjugation systems, operationally defined as machines that translocate DNA substrates intercellularly by a contact-dependent process; (ii) effector translocator systems, functioning to deliver proteins or other macromolecules to eukaryotic target cells; and (iii) DNA release/uptake systems, which translocate DNA to or from the extracellular milieu. Studies of a few paradigmatic systems, notably the conjugation systems of plasmids F, R388, RP4, and pKM101 and the Agrobacterium tumefaciens VirB/VirD4 system, have supplied important insights into the structure, function, and mechanism of action of type IV secretion machines. Information on these systems is updated, with emphasis on recent exciting structural advances. An underappreciated feature of T4SS, most notably of the conjugation subfamily, is that they are widely distributed among many species of gram-negative and -positive bacteria, wall-less bacteria, and the Archaea. Conjugation-mediated lateral gene transfer has shaped the genomes of most if not all prokaryotes over evolutionary time and also contributed in the short term to the dissemination of antibiotic resistance and other virulence traits among medically important pathogens. How have these machines adapted to function across envelopes of distantly related microorganisms? A survey of T4SS functioning in phylogenetically diverse species highlights the biological complexity of these translocation systems and identifies common mechanistic themes as well as novel adaptations for specialized purposes relating to the modulation of the donor-target cell interaction.

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Section: Cell Wall-less Systemsmentioning

confidence: 99%

Section: Cell Wall-less Systemsmentioning

confidence: 99%

Section: Cell Wall-less Systemsmentioning

confidence: 99%

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Biological Diversity of Prokaryotic Type IV Secretion Systems

Martı́nez

Christie

2009

Microbiol Mol Biol Rev

621

780

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“…Extrachromosomal forms of the integrative conjugative element have been shown in M. fermentans (Calcutt et al, 2002) and M. agalactiae (Marenda et al, 2006). A model has been proposed where the element is excised, at low frequency, from the chromosome, circularized as a nonreplicative intermediate, and transferred by conjugation to a recipient.…”

Section: Evidence For a Circular Intermediate Icehmentioning

confidence: 99%

“…ated by the excision from genome, like occurred in M. fermentans (Calcutt et al, 2002) and M. agalactiae (Marenda et al, 2006). The circular conformation of the excised ICEH has important implications for the biology of the element, since in this form, the constin can remain more stable, increasing the probability of horizontal transfers.…”

Section: Evidence For a Circular Intermediate Icehmentioning

confidence: 99%

Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae

et al. 2007

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Diversification of bacterial species and pathotypes is largely caused by lateral gene transfer (LGT) of diverse mobile DNA elements such as plasmids, phages, transposons and genomic islands. Thus, acquisition of new phenotypes by LGT is very important for bacterial evolution and relationship with hosts. This paper reports a 23 kb region containing fourteen CDSs with similarity to the previous described Integrative Conjugal Element of Mycoplasma fermentans (ICEF). This element, named ICEH, is present as one copy at distinct integration sites in the chromosome of 7448 and 232 pathogenic strains and is absent in the type strain J (non-pathogenic). Notable differences in the nucleotide composition of the insertion sites were detected, and could be correlated to a lack of specificity of the ICEH integrase. Although present in strains of the same organism, the ICEH elements are more divergent than the typical similarity between other chromosomal locus of Mycoplasma hyopneunomiae, suggesting an accelerated evolution of these constins or an ongoing process of degeneration, while maintaining conservation of the tra genes. An extrachromosomal form of this element had been detected in the 7448 strain, suggesting a possible involvement in its mobilization and transference of CDSs to new hosts.

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Molecular Biology Perspective of Susceptibility and Resistance in Main Target Pathogens in the Respective Species and Antimicrobials of Concern

Pokludová

2020

Antimicrobials in Livestock 1: Regulation, Science, Practice

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A New Integrative Conjugative Element Occurs in Mycoplasma agalactiae as Chromosomal and Free Circular Forms

Cited by 45 publications

References 23 publications

Biological Diversity of Prokaryotic Type IV Secretion Systems

Biological Diversity of Prokaryotic Type IV Secretion Systems

Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae

Molecular Biology Perspective of Susceptibility and Resistance in Main Target Pathogens in the Respective Species and Antimicrobials of Concern

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