A new insight to biomarkers related to resistance in survived-white spot syndrome virus challenged giant tiger shrimp,Penaeus monodon

Abstract

The emergence of diseases such as white spot disease has become a threat to Penaeus monodon cultivation. Although there have been a few studies utilizing RNA-Seq, the cellular processes of host-virus interaction in this species remain mostly anonymous. In the present study, P. monodon was challenged with WSSV by intramuscular injection and survived for 12 days. The effect of the host gene expression by WSSV infection in the haemocytes, hepatopancreas and muscle of P. monodon was studied using Illumina HiSeq 2000. The RNA-Seq of cDNA libraries was developed from surviving WSSV-challenged shrimp as well as from normal healthy shrimp as control. A comparison of the transcriptome data of the two groups showed 2,644 host genes to be significantly up-regulated and 2,194 genes significantly down-regulated as a result of the infection with WSSV. Among the differentially expressed genes, our study discovered HMGB, TNFSF and c-Jun in P. monodon as new potential candidate genes for further investigation for the development of potential disease resistance markers. Our study also provided significant data on the differential expression of genes in the survived WSSV infected P. monodon that will help to improve understanding of host-virus interactions in this species.

Transcriptomic analysis of the black tiger shrimp (Penaeus monodon) reveals insights into immune development in their early life stages

“…Cactus is phosphorylated, dislocated from Dorsal, and degraded, while NF-κB transcription factor Dorsal translocated into the nucleus to activate the expression of AMPs 28 , 78 . In P. monodon , Toll-like receptors (TLRs) have been reported as part of shrimp defense mechanisms against pathogen invasion 79 , 80 . Expression of TLR transcripts of P. monodon larva and adult are inducible upon exposure to V. harveyi 80 , 81 , suggesting their involvement in activating shrimp immune responses against pathogenic bacteria.…”

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

“…With the rapid development of high-throughput RNA sequencing technologies, including RNA sequencing (RNA-seq), more and more studies use RNA-seq to expose the immune molecular mechanism of shrimp against viral infections, bacterial infections, and environmental stress (Kurita and Nakajima, 2012;Wang et al, 2014;Zeng et al, 2014;Qin et al, 2018;Jin and Zhu, 2019;Nian et al, 2019;Duan et al, 2020). Among them, hemocytes were widely used in transcriptome studies of shrimp virus infections, such as WSSV, TSV, and DIV1 (Sookruksawong et al, 2013;Ghani and Bhassu, 2019;Wang et al, 2019;. Therefore, the immune molecular mechanism of shrimp against a virus infection can be better understood by using the transcriptome analysis of hemocytes combined with enzyme activity study.…”

Identification of Multigene Biomarker for Shrimp White Feces Syndrome by Full-Length Transcriptome Sequencing

“…Transcriptome analysis, which can be used to improve understanding of the genes underlying host response to disease, can also facilitate the identification of biomarkers for specific diseases as reported in compelling studies (Stockhammer et al, 2010;Zhu et al, 2017;Klett et al, 2018). A prior study identified three genes as new potential candidates for white spot syndrome virus resistance markers in giant tiger shrimp using transcriptome analysis (Mohd Ghani and Bhassu, 2019). One previous study identified a total of 24 key genes involved in multiple KEGG signaling pathways in Japanese flounder infected with Edwardsiella by sequencing the gill transcriptome and analyzing the gene interaction network .…”

“…Biomarkers contributing to the genetic resistance of the yellow catfish to bacterial infections were also established using transcriptome analysis . Similarly, prior studied used RNA-seq to uncover the transcriptome changes of Penaeus monodon that suffered from white spot syndrome virus and acute hepatopancreatic necrosis disease (Mohd Ghani and Bhassu, 2019;Soo et al, 2019). However, the research data cited above were generated by the second-generation sequencing technology, which meant that short-read sequence prevented the assembly of long transcripts (Xu et al, 2015).…”