A New Inhibitor of γ-aminobutric Acid Aminotransferase from Streptomyces sp. ZZ035 Isolated from a Folk Medicinal Soil in China

Abstract

Strain ZZ035 was isolated from a folk medicinal soil in China. Polyphasic taxonomy procedure indicated that this strain belonged to the genus Streptomyces and was closest to S. cinnamonensis with high similarities (99.2%) of 16S rDNA sequence. 1 was isolated from the broth of this strain and identified as 5-amonimethyl-2-iminoimidazolidine-4-carboxylic acid. Its chemical structure was established by its spectroscopic data, and 1 H and 13 C signals were assigned by its 1 H, 13C, Dept and HSQC spectra. Considering that 1 was a γ-aminobutric acid (GABA) derivative, its inhibitory activity against GABA-aminotransferase (GABA-AT) was assayed, and the result showed that 1 can inhibit GABA-AT activity with the 50% inhibitory concentration of approximately 60 μM. As 1 was a 2,3-disubstituted GABA derivative not published before, the approximate GABA-AT inhibitory activity of 1 compared to positive control vigabatrin showed that 1 were worthy of further research and developing.

Triglycerides Isolated from <i>Streptomyces</i> sp. ZZ035 and Their Nuclear Magnetic Resonance Spectroscopic Characters

“…Polyphasic taxonomy procedure indicated that this strain belonged to the genus Streptomyces and was closest to S. cinnamonensis [2]. Its 16S deoxyribonucleic acid (16S rDNA) sequence was deposited at NCBI GenBank with accession numbers KJ995739.…”

“…To reveal the anti-infection reasons of this soil, a sample was collected in Xianjing Countryside in Zhuzhou County, China, and sixty-one actinomycete strains ZZ01 to ZZ061 were selectively isolated from this sample [1]. After chemical analysis were performed for discovering strains producing a series of secondary metabolites, the bioactive evaluation showed that thirteen strains had antimicrobial activities against Staphylococcus aureus, Escherichia coli and/or Candida albicans, and then the classification and identification of targeted seven strains indicated that they belonged to the genus Streptomyces [2]. Among them, Streptomyces sp.…”