A New In Vivo Animal Model to Create Intervertebral Disc Degeneration Characterized by MRI, Radiography, CT/Discogram, Biochemistry, and Histology

Zhou, HaoWei; Hou, Shuxun; Shang, Wei-lin; Wu, Wenwen; Cheng, Yao I.; Mei, Fang; Peng, Baogan

doi:10.1097/01.brs.0000259835.31062.3d

Cited by 26 publications

(14 citation statements)

References 39 publications

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“…5,6 The decrease in T2 relaxation times with the stage of degeneration is associated with a decrease in water and glycosaminoglycan content in the disk. [15][16][17] The inverse correlation of T2 relaxation time in the disk and patient age has been reported by Jenkins et al 18 and Watanabe et al 14 We found a similar inverse correlation between age and relaxation time for grade II disks. In our study, the average T2 relaxation time decreased progressively by decades except for those in their 50s, probably due to small sample sizes.…”

Section: Discussionsupporting

confidence: 75%

T2 Relaxation Times Correlated with Stage of Lumbar Intervertebral Disk Degeneration and Patient Age

Marinelli¹,

Haughton²,

Anderson³

2010

AJNR Am J Neuroradiol

103

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Section: Discussionsupporting

confidence: 75%

T2 Relaxation Times Correlated with Stage of Lumbar Intervertebral Disk Degeneration and Patient Age

Marinelli¹,

Haughton²,

Anderson³

2010

AJNR Am J Neuroradiol

103

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“…It is suitable for documenting lesions of the AF . This technique can also specifically be used in an ovine model to characterize the ovine disc …”

Section: Discussionmentioning

confidence: 99%

“…27,34 This technique can also specifically be used in an ovine model to characterize the ovine disc. 25 As part of the clinical treatment of disc herniation with autologous chondrocytes, this procedure was successfully used to show closure of the AF before injecting the cells. 35 However, discography is an invasive method, as the disc is punctured with a needle and the contrast agent is injected.…”

Section: Discussionmentioning

confidence: 99%

Long‐Term Pathology of Ovine Lumbar Spine Degeneration Following Injury Via Percutaneous Minimally Invasive Partial Nucleotomy

Schwan

Ludtka

Friedmann

et al. 2019

Journal Orthopaedic Research

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The focus of this work is to assess the long‐term progression of degeneration in the ovine lumbar spine following a minimally invasive model injury comparable to the damage of an intervertebral disc (IVD) herniation. A partial nucleotomy was performed on 18 sheep via the percutaneous dorsolateral approach. The animals were culled at 6 and 12 months to evaluate the damaged and neighboring functional spine units (FSUs) for degenerative characteristics via μ‐CT and histology. Both quantitative μ‐CT and histology investigations demonstrated statistically significant differences between the native and damaged FSUs investigated. Qualitative analysis of μ‐CT revealed numerous pathological markers consistent with intervertebral disc degeneration (IDD), with differences in frequency and severity between the native and damaged FSUs. The annulus fibrosus reforms a pressure seal within 6 weeks, but the extent of the trauma is significant enough to initiate IVD degeneration, which is already clearly visible at 6 months and especially so 12 months post‐op. IDD pathology consistent with signs of a herniation was seen in both the 6‐ and 12‐month groups. This technique provides a useful model injury for the preclinical evaluation of IDD in large animal models, especially in regards to simulating disc herniation as well as for testing the efficacy of associated therapies in the future. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2376–2388, 2019

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“…Higher-grade degenerative processes often lead to clinically evident conditions that may require medical or surgical management. Current research attempts at investigating disc degeneration have focused on models thought to represent the late-stage degenerative changes [6-16]. Inevitably, induction of the degenerative cascade in these models requires the mechanical disruption of the AF and creating structural and morphological changes that mimic late degeneration [15-17].…”

Section: Introductionmentioning

confidence: 99%

An organ culture system to model early degenerative changes of the intervertebral disc

et al. 2011

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IntroductionBack pain, a significant source of morbidity in our society, is related to the degenerative changes of the intervertebral disc. At present, the treatment of disc disease consists of therapies that are aimed at symptomatic relief. This shortcoming stems in large part from our lack of understanding of the biochemical and molecular events that drive the disease process. The goal of this study is to develop a model of early disc degeneration using an organ culture. This approach is based on our previous studies that indicate that organ culture closely models molecular events that occur in vivo in an ex vivo setting.MethodsTo mimic a degenerative insult, discs were cultured under low oxygen tension in the presence of TNF-α, IL-1β and serum limiting conditions.ResultsTreatment resulted in compromised cell survival and changes in cellular morphology reminiscent of degeneration. There was strong suppression in the expression of matrix proteins including collagen types 1, 2, 6 and 9, proteoglycans, aggrecan and fibromodulin. Moreover, a strong induction in expression of catabolic matrix metalloproteinases (MMP) 3, 9 and 13 with a concomitant increase in aggrecan degradation was seen. An inductive effect on NGF expression was also noticed. Although similar, nucleus pulposus and annulus fibrosus tissues showed some differences in their response to the treatment.ConclusionsResults of this study show that perturbations in microenvironmental factors result in anatomical and gene expression change within the intervertebral disc that may ultimately compromise cell function and induce pathological deficits. This system would be a valuable screening tool to investigate interventional strategies aimed at restoring disc cell function.

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A New In Vivo Animal Model to Create Intervertebral Disc Degeneration Characterized by MRI, Radiography, CT/Discogram, Biochemistry, and Histology

Cited by 26 publications

References 39 publications

T2 Relaxation Times Correlated with Stage of Lumbar Intervertebral Disk Degeneration and Patient Age

T2 Relaxation Times Correlated with Stage of Lumbar Intervertebral Disk Degeneration and Patient Age

Long‐Term Pathology of Ovine Lumbar Spine Degeneration Following Injury Via Percutaneous Minimally Invasive Partial Nucleotomy

An organ culture system to model early degenerative changes of the intervertebral disc

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