A new immunomodulatory protein from Ganoderma microsporum inhibits epidermal growth factor mediated migration and invasion in A549 lung cancer cells

Lin, Ching‐Hsiung; Sheu, Gwo‐Tarng; Lin, Yu‐Wen; Yeh, Chin-Shui; Huang, Yu‐Hsin; Lai, Yen‐Chein; Chang, Jan-Gowth; Ko, Jiunn‐Liang

doi:10.1016/j.procbio.2010.06.006

Cited by 89 publications

(52 citation statements)

References 33 publications

(39 reference statements)

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“…and identified (Bastiaan-Net et al, 2013;Kino et al, 1989;Lin et al, 1997Lin et al, , 2010. Other six have been found in F. veltipes (Ko et al, 1995), V. volvacea , Auricularia polytricha (Sheu et al, 2004), Pleurotus citrinopileatus , Poria cocos (Chang et al, 2009) and Trametes versicolor (Li et al, 2011).…”

Section: Introductionmentioning

confidence: 95%

Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta

Shi²,

Ding

et al. 2015

Food and Chemical Toxicology

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Section: Introductionmentioning

confidence: 95%

Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta

Shi²,

Ding

et al. 2015

Food and Chemical Toxicology

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“…are attributed mainly to triterpenoids and polysaccharides, but more recently a new glycoprotein class in Ganoderma spp. named protein FIPs was identified as being immunomodulatory and anticancer [Lin et al, 2010]. Another report suggests that the mycelia of G. lucidum have high potential in lowering blood pressure level due to the presence of several antihypertensive proteins [Ansor et al, 2013].…”

Section: Proteomic Analysismentioning

confidence: 99%

Biochemical Characterization and Antioxidant and Antiproliferative Activities of Different <b><i>Ganoderma </i></b>Collections

et al. 2015

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The aim of this study was to conduct a molecular and biochemical characterization and to compare the antioxidant and antiproliferative activities of four Ganoderma isolates belonging to Ganoderma lucidum (Gl-4, Gl-5) and Ganoderma resinaceum (F-1, F-2) species. The molecular identification was performed by ITS and IGS sequence analyses and the biochemical characterization by enzymatic and proteomic approaches. The antioxidant activity of the ethanolic extracts was compared by three different methods and their flavonoid contents were also analyzed by high-performance liquid chromatography. The antiproliferative effect on U937 cells was determined by MTT assay. The studied mycelia differ both in the enzymatic activities and protein content. The highest content in total phenol and the highest antioxidant activity for DPPH free radical scavenging and chelating activity on Fe²⁺ were observed with the Gl-4 isolate of G. lucidum. The presence of quercetin, rutin, myricetin, and morin as major flavonoids with effective antioxidant activity was detected. The ethanolic extracts from mycelia of G. lucidum isolates possess a substantial antiproliferative activity against U937 cells in contrast to G. resinaceum in which the antiproliferative effects were insignificant. This study provides a comparison between G. lucidum and G. resinaceum mycelial strains, and shows that G. resinaceum could be utilized to obtain several bioactive compounds.

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“…Homologous cloning involves designing primers using known FIPs as templates and cloning FIPs in the same genus, so it is limited to identifying highly homologous proteins (identity>90%), making it difficult to further characterize FIP proteins. Until now, only five FIPs have been identified using the homologous cloning method: FIP-gmi [14], FIP-gsi from Ganoderma sinense [15], FIP-cru from Chroogomphis rutilus [16], FIP-gat from Ganoderma atrum [7], and LZ-9 [17]. Therefore, researchers have shifted away from the extraction or homologous cloning methods for identifying new FIPs and instead search for sequence similarity in the burgeoning genome databases.…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterisation of a Novel Protein FIP-sch3 from Stachybotrys chartarum

Zhao

et al. 2016

PLoS ONE

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In this study, a novel FIP named FIP-sch3 has been identified and characterised. FIP-sch3 was identified in the ascomycete Stachybotrys chartarum, making it the second FIP to be identified outside the order of Basidiomycota. Recombinant FIP-sch3 (rFIP-shc3) was produced in Escherichia coli and purified using GST-affinity magnetic beads. The bioactive characteristics of FIP-sch3 were compared to those of well-known FIPs LZ-8 from Ganoderma lucidum and FIP-fve from Flammulina velutipes, which were produced and purified using the same method. The purified rFIP-sch3 exhibited a broad spectrum of anti-tumour activity in several types of tumour cells but had no cytotoxicity in normal human embryonic kidney 293 cells. Assays that were implemented to study these properties indicated that rFIP-sch3 significantly suppressed cell proliferation, induced apoptosis and inhibited cell migration in human lung adenocarcinoma A549 cells. The anti-tumour effects of rFIP-sch3 in A549 cells were comparable to those of rLZ-8, but they were significantly greater than those of rFIP-fve. Molecular assays that were built on real-time PCR further revealed potential mechanisms related to apoptosis and migration and that underlie phenotypic effects. These results indicate that FIP-shc3 has a unique anti-tumour bioactive profile, as do other FIPs, which provide a foundation for further studies on anti-tumour mechanisms. Importantly, this study also had convenient access to FIP-sch3 with potential human therapeutic applications.

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A new immunomodulatory protein from Ganoderma microsporum inhibits epidermal growth factor mediated migration and invasion in A549 lung cancer cells

Cited by 89 publications

References 33 publications

Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta

Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta

Biochemical Characterization and Antioxidant and Antiproliferative Activities of Different <b><i>Ganoderma </i></b>Collections

Identification and Characterisation of a Novel Protein FIP-sch3 from Stachybotrys chartarum

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