A New IL‐1 Receptor Inhibitor 139A: Fermentation, Isolation, Physico‐Chemical Properties and Structure.

Chen, Jing; Wu, Jianbo; Li, Yuan; Jiang, Rong; Li, Baoyi

doi:10.1002/chin.200329165

Cited by 8 publications

(15 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…139 fermentation culture using the previously described protocol. 9 Western blot analysis FLSs were seeded at 1 3 10 5 /ml onto 6-well plates in DMEM and cultivated at 37 uC for 24 h, and various concentrations of ebosin in DMEM (assay for MAPK and NF-kB: 400, 80, 16, 3.2, 0.64, and 0.128 mg/ml; assay for IKK, IkB: 80, 16 and 3.2 mg/ml) were added and the cells were cultivated at 37 uC for an additional 12 h. IL-1b (10 ng/ml) was then added for 15 min (assay for MAPK) or 3 h (assay for IKK/NF-kB). After removing the supernatant through centrifugation (800g, 10 min), the cells were lysed in RIPA lysis buffer (Applygen) at 4 uC for 30 min.…”

Section: Isolation Of Ebosinmentioning

confidence: 99%

“…8 Ebosin, a novel exopolysaccharide (EPS) comprising rhamnose, fucose, arabinose, mannose, xylose, glucose, galactose and galacturonic acid, was isolated from the fermentation culture of Streptomyces sp. 139 9 and shown to significantly suppress the development of rat collagen-induced arthritis (CIA). 10 The remarkable anti-inflammatory effect of ebosin on CIA is mediated through the inhibition of IL-1b, IL-6 and TNF-a production at both transcriptional and posttranscriptional levels.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes

Zhang¹,

Wang²,

Bai³

et al. 2015

Cell Mol Immunol

View full text Add to dashboard Cite

The interleukin-1b-mitogen-activated protein kinase (MAPK) and NF-kB signaling pathways are involved in the pathogenesis of rheumatoid arthritis. Ebosin, a novel exopolysaccharide (EPS), exhibits anti-inflammatory activity in rat collagen-induced arthritis by suppressing the production of tumor necrosis factor-a, interleukin-6 and interleukin-1b. The aim of the present study was to assess the effects of ebosin on NF-kB and MAPK signaling pathways mediated through interleukin-1b in rat fibroblast-like synoviocytes (FLSs). Western blotting showed decreased production of phosphorylated p38, JNK1, JNK2, IKKa, IKKb and IkB in the cytoplasm and NF-kB in the nucleus upon ebosin treatment. The DNA-binding activity of NF-kB in the cell nucleus was also inhibited by ebosin treatment, as demonstrated using an electrophoresis mobility gel shift assay. Analysis of the results of the immunofluorescence assay also showed a reduced amount of NF-kB in the nucleus of cells affected by ebosin. These results provided evidence for the effects of ebosin on both interleukin-1b-mediated MAPK and NF-kB signaling pathways in rat FLSs. In addition, enzyme-linked immunosorbent assay demonstrated that ebosin reduces the levels of matrix metalloproteinases MMP-1 and MMP-3 and the chemokines, interleukin-8 and RANTES. Thus, the results of the present study provide further evidence for understanding the medicinal activity of ebosin at a molecular level, therefore nominating this EPS as a potential therapeutic candidate for the treatment of rheumatic arthritis.

show abstract

Section: Isolation Of Ebosinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes

Zhang¹,

Wang²,

Bai³

et al. 2015

Cell Mol Immunol

View full text Add to dashboard Cite

show abstract

“…139 (gtf ) were cultured at 28°C for 96 h respectively. Following the protocol as described before [9], Ebosin, EPS-7 m and EPS-7 g were isolated from the supernatants of fermentation cultures of respective strains.…”

Section: Isolation Of Epssmentioning

confidence: 99%

“…Recently a novel EPS namely Ebosin was isolated from the supernatants of fermentation cultures of Streptomyces sp. 139 [9], which has remarkable anti-rheumatic arthritis activity in vivo [10]. Its biosynthesis gene cluster (ste) consisting of 27 ORFs was also identified [11].…”

Section: Introductionmentioning

confidence: 99%

Characterization of an Ebosin derivative produced by heterologous gene replacement in

Zhang

Shan²,

Bao

et al. 2014

Microb Cell Fact

View full text Add to dashboard Cite

Background: Ebosin is a novel exopolysaccharide (EPS) produced by Streptomyces sp. 139 and evidenced to possess an anti-rheumatic arthritis activity in vivo. The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production. Aiming to generate derivatives of Ebosin for better activity, we replaced ste7 with a gene encoding for a glucosyltransferase (gtf) from Streptococcus thermophilus.Results: This alteration resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition dramatically changed, especially in the proportion of glucose which increased from 1.1% (Ebosin) to 84.01% (EPS-7 g). In an ELISA analysis, EPS-7 g exhibited a higher binding activity for IL-1R, as a competitor of interleukin-1, than that of Ebosin. It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS). In addition, experiments with acute inflamed mice induced by croton oil showed a significantly higher anti-inflammatory activity of EPS-7 g compared with Ebosin. Conclusions: The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments. This is the first report demonstrating a modification of EPS structure via heterologous gene replacement in Streptomyces.

show abstract

“…Ebosin contains galactose, arabinose, mannose, fucose, xylose, rhamnose, galacturonic acid and glucose. Its average molecular weight is 8.8 Â 10 5 Da and the structure is relatively complex [5]. Ebosin biosynthesis gene cluster (ste) consisting of 27 ORFs has been identified previously [6e8].…”

Section: Introductionmentioning

confidence: 99%

Biochemical characterization of a novel bifunctional glycosyl-1-phosphate transferase involved in the exopolysaccharide biosynthesis

Wang

et al. 2015

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

A New IL‐1 Receptor Inhibitor 139A: Fermentation, Isolation, Physico‐Chemical Properties and Structure.

Abstract: For Abstract see ChemInform Abstract in Full Text.

Cited by 8 publications

References 1 publication

Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes

Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes

Characterization of an Ebosin derivative produced by heterologous gene replacement in

Biochemical characterization of a novel bifunctional glycosyl-1-phosphate transferase involved in the exopolysaccharide biosynthesis

Contact Info

Product

Resources

About