A new hybrid scaffold constructed of solid freeform-fabricated PCL struts and collagen struts for bone tissue regeneration: fabrication, mechanical properties, and cellular activity

Ahn, Sang-Ho; Kim, YongBok; Lee, Hyeongjin; Kim, GeunHyung

doi:10.1039/c2jm33310d

Cited by 43 publications

(43 citation statements)

References 45 publications

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“…15 Unfortunately, bioinks which full this catalogue of requirements are currently not readily available. Plotted cell-free hydrogel structures with very well dened (pore) architecture have been reported, 16,17 however, inclusion of cells in the fabrication process generally comes at the cost of shape delity.…”

Section: Introductionmentioning

confidence: 99%

Development and characterisation of a new bioink for additive tissue manufacturing

et al. 2014

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Additive manufacturing forms a potential route towards economically viable production of cellular constructs for tissue engineering. Hydrogels are a suitable class of materials for cell delivery and 3D culture, but are generally unsuitable as construction materials. Gelatine-methacrylamide is an example of such a hydrogel system widely used in the field of tissue engineering, e.g. for cartilage and cardiovascular applications. Here we show that by the addition of gellan gum to gelatinemethacrylamide and tailoring salt concentrations, rheological properties such as pseudo-plasticity and yield stress can be optimised towards gel dispensing for additive manufacturing processes. In the hydrogel formulation, salt is partly substituted by mannose to obtain isotonicity and prevent a reduction in cell viability. With this, the potential of this new bioink for additive tissue manufacturing purposes is demonstrated.

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Section: Introductionmentioning

confidence: 99%

Development and characterisation of a new bioink for additive tissue manufacturing

et al. 2014

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“…4D). Previously, it has been shown that the compressive modulus of porous scaffolds reduces significantly as the porosity of the scaffolds increases [25,38,39]. For the same reason, the dual-pore scaffolds showed intermediate mechanical properties compared to structured and random pore scaffolds, indicating the expected relationship between porosity and the mechanical stiffness of the scaffolds.…”

Section: Mechanical Testingmentioning

confidence: 92%

Fabrication of scalable tissue engineering scaffolds with dual-pore microarchitecture by combining 3D printing and particle leaching

Mohanty

Sanger

Heiskanen

et al. 2016

Materials Science and Engineering: C

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a b s t r a c t a r t i c l e i n f oLimitations in controlling scaffold architecture using traditional fabrication techniques are a problem when constructing engineered tissues/organs. Recently, integration of two pore architectures to generate dual-pore scaffolds with tailored physical properties has attracted wide attention in tissue engineering community. Such scaffolds features primary structured pores which can efficiently enhance nutrient/oxygen supply to the surrounding, in combination with secondary random pores, which give high surface area for cell adhesion and proliferation. Here, we present a new technique to fabricate dual-pore scaffolds for various tissue engineering applications where 3D printing of poly(vinyl alcohol) (PVA) mould is combined with salt leaching process. In this technique the sacrificial PVA mould, determining the structured pore architecture, was filled with salt crystals to define the random pore regions of the scaffold. After crosslinking the casted polymer the combined PVA-salt mould was dissolved in water. The technique has advantages over previously reported ones, such as automated assembly of the sacrificial mould, and precise control over pore architecture/dimensions by 3D printing parameters. In this study, polydimethylsiloxane and biodegradable poly(ϵ-caprolactone) were used for fabrication. However, we show that this technique is also suitable for other biocompatible/biodegradable polymers. Various physical and mechanical properties of the dual-pore scaffolds were compared with control scaffolds with either only structured or only random pores, fabricated using previously reported methods. The fabricated dual-pore scaffolds supported high cell density, due to the random pores, in combination with uniform cell distribution throughout the scaffold, and higher cell proliferation and viability due to efficient nutrient/oxygen transport through the structured pores. In conclusion, the described fabrication technique is rapid, inexpensive, scalable, and compatible with different polymers, making it suitable for engineering various large scale organs/tissues.

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“…Not only does it provide cells with a physical support, studies have also shown that a mechanical strength which matches in vivo conditions can strongly influence cell proliferation and differentiation [56][57][58] . Several researchers have used a hybrid material approach to create a mechanically suitable environment [59,60] . However, the printability, from changes in viscosities and a mismatch in the most suitable printing technique for the materials, must be assessed and thus further adds to the complexity of the issue.…”

Section: Mechanical Strength and Structural Integritymentioning

confidence: 99%

3D bioprinting for tissue engineering: Stem cells in hydrogels

Mehrban¹,

Teoh²,

Birchall³

2024

IJB

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Surgical limitations require alternative methods of repairing and replacing diseased and damaged tissue. Regenerative medicine is a growing area of research with engineered tissues already being used successfully in patients. However, the demand for such tissues greatly outweighs the supply and a fast and accurate method of production is still required. 3D bioprinting offers precision control as well as the ability to incorporate biological cues and cells directly into the material as it is being fabricated. Having precise control over scaffold morphology and chemistry is a significant step towards controlling cellular behaviour, particularly where undifferentiated cells, i.e., stem cells, are used. This level of control in the early stages of tissue development is crucial in building more complex systems that morphologically and functionally mimic in vivo tissue. Here we review 3D printing hydrogel materials for tissue engineering purposes and the incorporation of cells within them. Hydrogels are ideal materials for cell culture. They are structurally similar to native extracellular matrix, have a high nutrient retention capacity, allow cells to migrate and can be formed under mild conditions. The techniques used to produce these materials, as well as their benefits and limitations, are outlined.

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A new hybrid scaffold constructed of solid freeform-fabricated PCL struts and collagen struts for bone tissue regeneration: fabrication, mechanical properties, and cellular activity

Cited by 43 publications

References 45 publications

Development and characterisation of a new bioink for additive tissue manufacturing

Development and characterisation of a new bioink for additive tissue manufacturing

Fabrication of scalable tissue engineering scaffolds with dual-pore microarchitecture by combining 3D printing and particle leaching

3D bioprinting for tissue engineering: Stem cells in hydrogels

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