A new human monoclonal antibody to rhesus blood group E antigen. Useful Typing Reagent for E-positive red cells.

吉源, 権; 勇一, 刀根; 奈留美, 須合; 孔, 宮崎; 俊明, 加藤; 久實, 池田; 定美, 関口

doi:10.3925/jjtc1958.38.446

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Cell Transformation and Fusion1

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1994

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“…Peripheral blood lymphocytes from a healthy female blood donor whose serum antibody showed anti-Jr" specificity with a titer of 1:128 in antiglobulintestwereisolated by density gradient centrifugation on performed as described previously [13]. In short, EBV-transformed they were in general [481 * The study in vivO using 51 Cr-labelled red Ficoll-Conray.…”

Section: Cell Transformation and Fusionmentioning

confidence: 99%

A Human Monoclonal Antibody to High‐Frequency Red Cell Antigen Jr^a

Miyazaki¹,

Kwon

Yamamoto

et al. 1994

Vox Sanguinis

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A human-mouse heterohybridoma (HMR0921) secreting human monoclonal IgG3, lambda antibody was produced from peripheral blood lymphocytes of a healthy blood donor with serum antibody to Jra, by EBV transformation and hybridization with mouse myeloma cell line P3X63Ag8.653. The reactivity of HMR0921 antibody was assessed by antiglobulin test with a panel of red cells including 14 different rare blood types. Only Jr(a-) red cells were negative. The strict specificity of this antibody to Jra antigen was further confirmed by absorption test with fluorescence flow cytometry. On screening of 28,744 blood donor samples by HMR0921 antibody, we detected 19 agglutination-negative samples, which were confirmed as Jr(a-) by conventional anti-Jra antisera. Therefore, our HMR0921 antibody is extremely useful for detecting rare Jr(a-) blood.

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Section: Cell Transformation and Fusionmentioning

confidence: 99%