A new human breast cancer cell line, KPL-1 secretes tumour-associated antigens and grows rapidly in female athymic nude mice

Abstract

We recently established a new human breast cell line, designated KPL-1, which was derived from the malignant effusion of a patient with breast cancer. This cell line is highly tumorigenic and grows rapidly in female nude mice. Cytogenetic analysis indicated its human origin and revealed a hypertriploid modal number of chromosomes. Electron microscopic examination suggested that the KPL-1 cells are of epithelial origin. Immunohistochemical studies revealed that the cells express cytokeratin, carcinoembryonic antigen and CA 15-3. They also possess a large number of oestrogen receptors but not progesterone receptors. Interestingly, KPL-1 cells seem to grow oestrogen independently in vitro. No amplification of c-erbB-2, c-myc, H-ras and N-ras genes was detected. KPL-1 cells secrete a large amount of tissue polypeptide antigen (TPA). Although the secretion of CA 15-3 seemed to be constant throughout all cell growth phases, TPA secretion increased during the exponential growth phase and decreased during the plateau phase. Serum TPA levels significantly correlated with the volume of KPL-1 tumours transplanted into nude mice. These data suggest that this KPL-1 cell line may be useful for studying oestrogen-independent growth and the kinetics of tumour-associated antigens in vivo as well as in vitro. Images Figure 1 Figure 2 Figure 3 Figure 5 Figure 9

Additive antitumour effect of the epidermal growth factor receptor tyrosine kinase inhibitor gefitinib (Iressa, ZD1839) and the antioestrogen fulvestrant (Faslodex, ICI 182,780) in breast cancer cells

“…KPL-1, KPL-3C and KPL-4 human breast cancer cell lines were established in our laboratory (Kurebayashi et al, 1995;Kurebayashi et al, 1996;Kurebayashi et al, 1999). KPL-1 cells are derived from a postmenopausal patient with hormone-refractory breast cancer.…”

A new human breast cancer cell line, KPL-3C, secretes parathyroid hormone-related protein and produces tumours associated with microcalcifications in nude mice

“…DNA dot -blot hybridisation was performed as previously described (Kurebayashi et al, 1995). Briefly, DNA samples were spotted onto Hybond N nylon sheets (Amersham, Arlington Heights, IL, USA) using a Hybri-dot blotting manifold (BRL, Bethesda, MD, USA).…”

“…These radiographic findings suggest that the microcalcifications of the transplanted tumours are very similar to those commonly observed in human breast cancer. Three transplanted tumours injected with other cell lines (MCF-7, MKL-4 or KPL-1) (Soule et al, 1973;Kurebayashi et al, 1993Kurebayashi et al, 1995 were also radiographed by the same method. No such microcalcifications were observed in those tumours.…”

A Radicicol Derivative, KF58333, Inhibits Expression of Hypoxia‐inducible Factor‐1α and Vascular Endothelial Growth Factor, Angiogenesis and Growth of Human Breast Cancer Xenografts

“…16) To clarify if a radicicol derivative, KF58333, has anti-angiogenic activity, it was administered to two human breast cancer cell lines, KPL-1 and KPL-4. 17,18) The findings of the present study show that this agent significantly inhibits vascular endothelial growth factor (VEGF) expression in vitro and angiogenesis in vivo. VEGF expression is reported to be partly regulated by the hypoxia-inducible factor (HIF)-1 signaling pathway.…”

“…The characteristics of these cell lines was reported elsewhere. 17,18) In brief, the KPL-1 cell line expresses a high level of estrogen receptors and a low level of HER2. The KPL-4 cell line expresses a low level of estrogen receptors and a high level of HER2.…”