A new HPTLC platformed luminescent biosensor system for facile screening of captan residue in fruits

Chen, Yisheng; Huang, Chao; Hellmann, Bernhard; Jin, Zhengyu; Xu, Xueming; Xiao, Guosheng

doi:10.1016/j.foodchem.2019.125691

Cited by 11 publications

(7 citation statements)

References 26 publications

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“…Hence, in order to determine the number of compounds, and HPTLC analysis on the ethanolic extract of the leaf, stem as well as roots were conducted. HPTLC is a valuable tool for the investigation of herbal products with respect to different aspects of their quality 12 . The majority of plant ingredients react with vanillinsulphuric acid with colored zones; therefore this spray reagent was used to detect the presence of phytocompounds through HPTLC of G. pentaphylla leaf extracts.…”

Section: Resultsmentioning

confidence: 99%

Untitled

2021

IJPSR

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Medicinal plants are often used by humankind since their evolution. They are known to have prominent therapeutic potential. The present work is conducted on Glycosmis pentaphylla (Rutaceae) an ethnobotanically important medicinal plant rich in secondary metabolites. The plant possesses metabolites like alkaloids, flavonoids, terpenoids, etc., which can be used against various illnesses. The plant is traditionally used by the folklore people in many parts of the world. The aim of the study is to investigate the bioactive compounds present and to evaluate the significance of the therapeutic and pharmacological uses of the phytoconstituents. All the standard phytochemical and spectroscopic procedures were followed for the detection and estimation of phytoconstituents. The leaves, stem, and root of Glycosmis pentaphylla were collected and was extracted using various solvents based on their polarity. Preliminary phytochemical analysis reveals the occurrence of several secondary metabolites. The quantitative estimation exhibited the presence of secondary metabolites abundantly in leaves on comparison with stem and root. Further, an HPTLC analysis to determine the number of phytoconstituents was also conducted on the leaf extract. The preliminary and HPTLC results revealed the presence of more constituents on the leaf extract, and hence, this was further subjected to an FT-IR analysis to determine the major functional group in the crude extract. From this study, it can be concluded that the ethnomedicinal plant Glycosmis pentaphylla contains various bioactive compounds. Further phytochemical and pharmacological studies are an urgent need for the isolation and discovery of some novel drugs to cure several disorder.

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Section: Resultsmentioning

confidence: 99%

Untitled

2021

IJPSR

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“…The bioluminescent bacteria strain Photobacterium phosphoreum was provided by Nanjing Institute of Soil Science, Chinese Academy of Sciences. The method of preparing bacterial suspension with bioluminescence was principally based on the steps previously described [ 23 , 24 ]. When strong greenish bioluminescence became observable to eye inspection, as shown in Figure 1 , the bacterial suspension was diluted with another 100 mL liquid medium prior to usage.…”

Section: Methodsmentioning

confidence: 99%

“…This enabled the analyst to expand the capacity of HPTLC by easily integrating a large array of assays that conventionally performed interpedently, such as surface enhanced Raman spectroscopy [ 15 , 16 , 17 ] and IR [ 18 , 19 ]. Particularly, HPTLC separation in combination with elaborately chosen effect-directed assays, also termed bioautography, had been demonstrated a novel and promising tool for screening of compounds with specific bioactivity in bio-mixtures [ 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

HPTLC-Bioluminescent Bioautography Screening of Herbal Teas for Adulteration with Hypolipidemic Drugs

Wang

et al. 2023

Biosensors

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Teas based on nutraceutical herbs are an effective tool against hyperlipidemia. However, the adulteration with chemical drugs is frequently detected. By coupling bioluminescent bioautography with high performance thin-layer chromatography (HPTLC), we developed a facile method suitable for screening hypolipidemic drugs (ciprofibrate and bezafibrate) adulteration in five different herbal teas (lotus leaf, Apocynum, Ginkgo biloba, Gynostemia and chrysanthemum). First, the sensitivity of a bioluminescent bacteria to the analyte was evaluated on different HPTLC layer materials, revealing that the best performance was achieved on the silica gel layer. On this basis, sample extracts were separated on silica gel plates via a standardized HPTLC procedure, forming a selective detection window for the targeted compound. Then, the separation results were rapidly visualized by the bioluminescence inhibition of bacteria cells within 6 min after dipping. The observed inhibition displayed an acceptable limit of detection (<20 ng/zone or 2 mg/kg) and linearity (R2 ≥ 0.9279) within a wide concentration range (50–1000 ng/zone). Furthermore, the optimized method was performed with artificially adulterated samples and the recovery rates were determined to be within the range of 71% to 91%, bracing its practical reliability. Showing superiorly high simplicity, throughput and specificity, this work demonstrated that the analytical method jointly based on HPTLC and bioautography was an ideal tool for screening bioactive compounds in complex biological matrix.

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“…It displays outstandingly broader detection spectrum compared to those of the other bioautography based on enzyme reactions. More specifically, any chemicals causing the up/downregulation of cell bioluminescence could be documented [31] . Since they are mostly isolated from the sea/lake water, bioluminescent bacteria will not be associated with safety and availability problems compared to those genetically modified microorganism [18,[32][33] .…”

Section: Food Science and Human Wellnessmentioning

confidence: 99%

“…Since they are mostly isolated from the sea/lake water, bioluminescent bacteria will not be associated with safety and availability problems compared to those genetically modified microorganism [18,[32][33] . When jointly used with HPTLC, bioluminescent bacteria have been successfully applied for detection multi-class veterinary drugs and pesticides, displaying satisfactory usability [31,34] . More importantly, the instant change of bioluminescence emission when exposed to bioactive compounds staying on the HPTLC platform shortens the visualization to minutes, further increasing the applicability of this combination.…”

Section: Food Science and Human Wellnessmentioning

confidence: 99%

Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC

Chen¹,

Xi²

2024

Food Science and Human Wellness

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As a widely used food preservative, methyl paraben was experimentally evidenced with serious hormonelike adverse effects. Herein, a high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantifi cation and confi rmation of methyl paraben was proposed and validated in vinegar and coconut juice. First, the detectability of the bioautography to the analyte on different layer materials was estimated, revealing that normal silica gel was the best choice. After that, the liquid of sample extract and working solution were separated to overcome the background noises due to co-extracted matrices. The separation result was then coupled to the optimized bioautography, enabling instant and straightforward screening of the targeted compound. For accurate quantifi cation, bioluminescent inhibition pattern caused by the analyte was processed by image analysis, giving useful sensitivity (LOD > 16 mg/kg), precision (RSD < 10.1%) and accuracy (spike-recovery rate 76.9%-112.2%). Finally, the suspected result was confi rmed by determining its MS fi ngerprint, further strengthening the reliability of screening.

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A new HPTLC platformed luminescent biosensor system for facile screening of captan residue in fruits

Cited by 11 publications

References 26 publications

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HPTLC-Bioluminescent Bioautography Screening of Herbal Teas for Adulteration with Hypolipidemic Drugs

Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC

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