A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas

Moutailler, Sara; Yousfi, Lena; Mousson, Laurence; Devillers, Elodie; Vazeille, Marie; Vega-Rúa, Anubis; Perrin, Yvon; Jourdain, Frédéric; Chandre, Fabrice; Cannet, Arnaud; Chantilly, Sandrine; Restrepo, Johana; Guidez, Amandine; Dusfour, Isabelle; Abreu, Filipe Vieira Santos de; Santos, Taissa Pereira dos; Jiolle, Davy; Visser, Tessa M.; Koenraadt, Constantianus J. M.; Wongsokarijo, Merril; Diallo, Mawlouth; Diallo, Diawo; Gaye, Alioune; Boyer, Sébastien; Duong, Veasna; Piorkowski, Géraldine; Paupy, Christophe; Oliveira, Ricardo Lourenço de; Lamballerie, Xavier de; Failloux, Anna‐Bella

doi:10.3390/v11100904

Cited by 24 publications

(39 citation statements)

References 35 publications

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“…aegypti females were stored at − 80 °C in Eppendorf tubes that contained silica gel grains and a small tray of Whatman filter paper to prevent water damage and degradation of the mosquitoes. They were sent to the Animal Health Laboratory, ANSES (Maisons-Alfort, France) for virus detection, see reference [45] for more details on the findings.…”

Section: Experimental Designmentioning

confidence: 99%

Optimisation and field validation of odour-baited traps for surveillance of Aedes aegypti adults in Paramaribo, Suriname

Visser

Cock

Hiwat³

et al. 2020

Parasites Vectors

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Background: Emerging arboviral diseases like Zika, dengue and chikungunya that are transmitted by Aedes aegypti mosquitoes, are increasingly threatening human health. Blends of human-like synthetic chemical attractants can be used to attract host-seeking mosquitoes. The aim of this study was to test new combinations of traps and odour baits in the laboratory, followed by testing the best candidates in the field to improve Ae. aegypti monitoring and surveillance.Methods: First, the BG-Suna trap was evaluated for capturing laboratory-reared Ae. aegypti by testing normal and inverted positions in screen cage tests. Secondly, the attractiveness of the MB5 blend, CO 2 , and their combination was tested. Thirdly, we tested the attractiveness of different trap types (BG-Suna, BG-Sentinel, MM-X and CDC light trap). Finally, we confirmed laboratory results in the field in Paramaribo, Suriname, using the MB5 and BG-Lure odour blends, CO 2 and the BG-Sentinel and BG-Bowl trap using a Latin Square design. Results:The MB5 blend in combination with CO 2 outperformed traps baited only with CO 2 or MB5 in screen cage tests (P < 0.0001). The BG-Sentinel trap performed equally well as the inverted BG-Suna and was taken to the field (P = 0.729). In the field, we captured Ae. aegypti, Cx. quinquefasciatus and Cx. nigripalpus. We confirmed the laboratory results and found that the combination of the MB5 blend and CO 2 almost doubled Ae. aegypti female captures (P = 0.004) and more than doubled Culex spp. female captures (P = 0.005) compared to using only CO 2 . Interestingly, the MB5 blend outperformed the commercially available BG-Lure, in the BG-Sentinel (P < 0.001). The BG-Bowl also attracted Ae. aegypti when baited with the MB5 blend in similar numbers as the BG-Sentinel baited with the MB5 (P = 0.362). Conclusions:Our study demonstrated that the BG-Sentinel trap baited with the MB5 blend and CO 2 outperforms the current golden standard (BG-Sentinel trap with BG-Lure) for monitoring Ae. aegypti females and males, in both laboratory and field experiments. The BG-Bowl baited with the MB5 blend is a good candidate for home use. Finally, the results show that CO 2 is an indispensable component of the attractive blend.

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Section: Experimental Designmentioning

confidence: 99%

Optimisation and field validation of odour-baited traps for surveillance of Aedes aegypti adults in Paramaribo, Suriname

Visser

Cock

Hiwat³

et al. 2020

Parasites Vectors

Self Cite

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“…The fundamental feature of the microfluidic chip is the integration of functional modules. The major advantages of this technology are high efficiency, high density, high throughput and low consumption [25][26][27][28]. Compared with the traditional PCR method, the microfluidic chip is much smaller in size and more portable, uses much less reagents, changes temperature more rapidly, integrates with other devices more easily, and has more moderate demands for experimental environment and lab assistants.…”

Section: Introductionmentioning

confidence: 99%

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Jiang¹,

Jiang

et al. 2020

Preprint

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KEYWORDS 2 swine disease, microfluidic chips, PCR, multiplex and on-site detection 3 Abstract Background: At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process takes a rather long time, has the risks of degradation of biological samples and generation of pathogenic microorganisms, and does not meet the rapid on-site detection demand. Therefore, development of a technology for rapid and high-throughput detection of pathogenic microorganisms at the customs port is of great significance. This study was to develop a microfluidic chip to be applied to rapid high-throughput detection for swine disease with higher accuracy and lower risk of spreading pathogenic microorganisms during transportation. Results: PCR technology has the advantages of high accurate and sensitivity in disease detection, clinical testing and food quarantine, so it plays an important role in customs inspection. However, the traditional PCR detection instrument has a large size, is timeconsuming and has strict requirements on the experimental environment, which greatly limit its application in on-site testing. In this paper, the positive nucleic acid of four swine diseases were detected by a portable and rapid microfluidic PCR system, which could achieve a on-site real-time quantitative PCR detection. Eight clinical samples were detected together on the microfluidic chip in the system, and the detection results were obtained in about an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results show that the high sensitivity and specificity of the microfluidic PCR detection system in disease detection will play an important role in customs inspection and quarantine during customs clearance. Conclusion: The microfluidic PCR detection system established in this study could meet the requirements for rapid detection of samples at the customs port The new method can avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reducing the inspection cycle, and would enable parallel inspections on one chip which greatly raising the efficiency of inspection.Corporation for their support of chip design and fabrication and microchip based PCR detection instrument. Authors' contributionsJY designed and directed the study. JS and JY perform the experiment ,collect results and analyze data. YW and BZ draft the first vision of the manuscript. KMW, CG and DXZ collect clinical serum samples. BZ critically revised the manuscripts. All the participants carefully reviewed this vision manuscript. Ethics approval and consent to participate 11Not applicable Consent for publicationNot applicable Competing interest. Publisher's NoteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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“…The fundamental feature of the microfluidic chip is the integration of functional modules. The major advantages of this technology are high efficiency, high density, high throughput and low consumption [23][24][25][26]. Compared with the traditional PCR method, the microfluidic chip is much smaller in size and more portable, uses much less reagents, changes temperature more rapidly, integrates with other devices more easily, and has more moderate demands for experimental environment and lab assistants.…”

Section: Introductionmentioning

confidence: 99%

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Zhou

Jiang²,

et al. 2020

Preprint

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Background：At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process takes a rather long time, has the risks of degradation of biological samples [32] and generation of pathogenic microorganisms [33], and does not meet the rapid on-site detection demand [34]. Therefore, development of a technology for rapid and high- throughput detection of pathogenic microorganisms at the customs port is of great significance. This study was to develop a microfluidic chip to be applied to rapid high-throughput swine disease detection with higher accuracy and lower risk of spreading pathogenic microorganisms during transportation.Results: PCR technology has the advantages of high accurate and sensitivity in disease detection, clinical testing and food quarantine, so it plays an important role in customs inspection. However, the traditional PCR detection instrument has a large size, is time-consuming and has strict requirements on the experimental environment, which greatly limit its application in on-site testing. In this paper, the clinical samples of four swine diseases were detected by a portable and rapid microfluidic PCR system, which could achieve a on- site real-time quantitative PCR detection. Eight clinical samples were detected together on the microfluidic chip in the system, and the detection results were obtained in about an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results show that the high sensitivity and specificity of the microfluidic PCR detection system in disease detection will play an important role in customs inspection and quarantine during customs clearance.Conclusion: The microfluidic PCR detection system established in this study could meet the requirements for rapid detection of samples at the customs portThe new method can avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reducing the inspection cycle, and would enable parallel inspections on one chip which greatly raising the efficiency of inspection.

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A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas

Cited by 24 publications

References 35 publications

Optimisation and field validation of odour-baited traps for surveillance of Aedes aegypti adults in Paramaribo, Suriname

Optimisation and field validation of odour-baited traps for surveillance of Aedes aegypti adults in Paramaribo, Suriname

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

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