A new high-molecular-weight glutenin subunit from the slovak wheat (Triticum aestivum L.) cultivar ‘Trebišovská 76’

Mihálik, Daniel; Nogová, Ľubica; Ondreičková, Katarína; Gubišová, Marcela; Gubiš, Jozef; Gregová, E.; Dokupilová, Ivana; Drška, Roman; Kraic, Ján

doi:10.1007/s10068-013-0045-5

Cited by 5 publications

(5 citation statements)

References 19 publications

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“…6 is similar to the sequence EU266533 from Slovak wheat variety ‘Trebišovská 76’, with identity of 99.70%, and 1Dy12 . 7 is similar to the sequence JF736016 from wheat cultivar Shinchunaga (97.44%) [ 44 ]. The length of both 1Dy12 .…”

Section: Resultsmentioning

confidence: 77%

“…The length of both 1Dy12 . 6 and EU266533 sequences is 2022 bp, and longer than other sequences of 1Dy allele ( Table 1 ) [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

“…The HMW-GSs encoded by the Glu-1D locus are found to be responsible for major wheat dough quality variances, particularly elasticity and strength, and have been successfully employed in wheat quality improvement [ 42 , 43 ]. Up to now, only five active Glu-1Dy genes have been cloned and characterized from bread wheat [ 44 ]. Recently, we characterized the allelic variation at the Glu-1 locus in 485 Chinese wheat landraces using MALDI-TOF-MS method, and identified several novel Glu-1 subunits [ 41 ].…”

Section: Introductionmentioning

confidence: 99%

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Two Novel Y-Type High Molecular Weight Glutenin Genes in Chinese Wheat Landraces of the Yangtze-River Region

Peng

Zhang

et al. 2015

PLoS ONE

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High molecular weight glutenin subunits (HMW-GSs) are key determinants for the end-use quality of wheat. Chinese wheat landraces are an important resource for exploring novel HMW-GS genes to improve the wheat baking quality. Two novel Glu-1Dy HMW-GSs (designated as 1Dy12.6 and 1Dy12.7) were identified and cloned from two Chinese wheat landraces Huazhong830 and Luosimai. The 1Dy12.6 and 1Dy12.7 subunits were deposited as the NCBInr Acc. No KR262518, and KR262519, respectively. The full open reading frames (ORFs) of 1Dy12.6 and 1Dy12.7 were 2022 bp and 1977 bp, encoding for proteins of 673 and 658 amino acid residues, respectively. Each contains four typical primary regions of HMW-GSs (a signal peptide, N- and C-terminal regions, and a central repetitive region). Their deduced molecular masses (70,165 Da and 68,400 Da) were strikingly consistent with those identified by MALDI-TOF-MS (69,985Da and 68,407 Da). The 1Dy12.6 is the largest 1Dy glutenin subunits cloned in common wheat up to date, containing longer repetitive central domains than other 1Dy encoded proteins. In comparison with the most similar active 1Dy alleles previously reported, the newly discovered alleles contained a total of 20 SNPs and 3 indels. The secondary structure prediction indicated that 1Dy12.6 and 1Dy12.7 have similar proportion of α-helix, β-turn, and β-bend to those of 1Dy10 (X12929). The phylogenetic analysis illustrated that the x- and y-type subunits of glutenins were well separated, but both 1Dy12.6 and 1Dy12.7 were clustered with the other Glu-1Dy alleles. Our results revealed that the 1Dy12.6 and 1Dy12.7 subunit have potential to strengthen gluten polymer interactions, and are valuable genetic resources for wheat quality improvement.

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Section: Resultsmentioning

confidence: 77%

“…The length of both 1Dy12 . 6 and EU266533 sequences is 2022 bp, and longer than other sequences of 1Dy allele ( Table 1 ) [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Two Novel Y-Type High Molecular Weight Glutenin Genes in Chinese Wheat Landraces of the Yangtze-River Region

Peng

Zhang

et al. 2015

PLoS ONE

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“…High molecular weight glutenin subunits of wheat determineits unique dough properties and also baking performance. With the development in proteomics, some authors [ 7 , 24 ] have identified new HMW-GS using electrophoretic and mass spectrometry analysis, but other authors [ 35 , 36 ] also reported novel HMW-GS using mostly DNA analysis. In this study, novel HMW-GS was identified using the most popular electrophoretic methods such as SDS-PAGE and 2D-PAGE.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Novel x-Type HMW Glutenin Subunit 1B × 6.5 in Wheat

Hlozáková

Gálová

Šliková

et al. 2021

Plants

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A novel high molecular weight glutenin subunit encoded by the Glu-1B locus was identified in the French genotype Bagou, which we named 1B × 6.5. This subunit differed in SDS-PAGE from well-known 1B × 6 and 1B × 7 subunits, which are also encoded at this locus. Subunit 1B × 6.5 has a theoretical molecular weight of 88,322.83 Da, which is more mobile than 1B × 6 subunit, and isoelectric point (pI) of about 8.7, which is lower than that for 1B × 6 subunit. The specific primers were designed to amplify and sequence 2476 bp of the Glu-1B locus from genotype Bagou. A high level of similarity was found between the sequence encoding 1B × 6.5 and other x-type encoding alleles of this locus.

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“…Such genes occupy approximately about one-fifth of all protein-encoding genes within plant genomes [ 30 ]. They include housekeeping genes, for example, genes encoding key enzymes included in the primary metabolism, storage proteins, and other proteins [ 31 , 32 ]. The fact that the EeDAcT gene does not contain introns emphasizes its importance in plant metabolism and plant life.…”

Section: Discussionmentioning

confidence: 99%

Diacylglycerol Acetyltransferase Gene Isolated from Euonymus europaeus L. Altered Lipid Metabolism in Transgenic Plant towards the Production of Acetylated Triacylglycerols

Mihálik

Lančaričová

Mrkvová

et al. 2020

Life

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Euonymus species from the Celastraceae family are considered as a source of unusual genes modifying the oil content and fatty acid composition of vegetable oils. Due to the possession of genes encoding enzyme diacylglycerol acetyltransferase (DAcT), Euonymus plants can synthesize and accumulate acetylated triacyglycerols. The gene from Euonymus europaeus (EeDAcT) encoding the DAcT was identified, isolated, characterized, and modified for cloning and genetic transformation of plants. This gene has a unique nucleotide sequence and amino acid composition, different from orthologous genes from other Euonymus species. Nucleotide sequence of original EeDAcT gene was modified, cloned into transformation vector, and introduced into tobacco plants. Overexpression of EeDAcT gene was confirmed, and transgenic host plants produced and accumulated acetylated triacylglycerols (TAGs) in immature seeds. Individual transgenic plants showed difference in amounts of synthesized acetylTAGs and also in fatty acid composition of acetylTAGs.

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A new high-molecular-weight glutenin subunit from the slovak wheat (Triticum aestivum L.) cultivar ‘Trebišovská 76’

Cited by 5 publications

References 19 publications

Two Novel Y-Type High Molecular Weight Glutenin Genes in Chinese Wheat Landraces of the Yangtze-River Region

Two Novel Y-Type High Molecular Weight Glutenin Genes in Chinese Wheat Landraces of the Yangtze-River Region

Molecular Characterization of Novel x-Type HMW Glutenin Subunit 1B × 6.5 in Wheat

Diacylglycerol Acetyltransferase Gene Isolated from Euonymus europaeus L. Altered Lipid Metabolism in Transgenic Plant towards the Production of Acetylated Triacylglycerols

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