A new growth medium for virulent <i>Bordetella pertussis</i>

Sutherland, Ian W.; Wilkinson, John F.

doi:10.1002/path.1700820220

Cited by 24 publications

(7 citation statements)

References 12 publications

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“…The strains used were: Bordetella pertusSis, NCTC 8474, 4412 Media. Virulent BordeteZZa pertussis strains were grown in the tris + resin liquid medium described in an earlier paper (Sutherland & Wilkinson, 1961). Other species were grown in a medium which contained (quantities/l.…”

Section: Methodsmentioning

confidence: 99%

Azurin: A Copper Protein Found in Bordetella

Sutherland

Wilkinson

1963

Journal of General Microbiology

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SUMMARYAll strains of Bordetella pertussis, B. bronchiseptica and B. parapertussis tested, were found to contain a blue protein; this has been named ' Azurin'. It has been extracted from the cells of all three species and it has also been isolated from culture supernatant fluids of B . pertussis. The azurin was purified by chromatography on ion-exchange cellulose derivatives and obtained in physically pure form; the materials from each species were identical. In one strain of B. bronchiseptica azurin comprised 0.1 yo of the bacterial dry weight. Azurin is a water-soluble autoxidizable protein of high oxidation-reduction potential (+a95 mV.). It has an absorption maximum at 625 mp in the oxidized form, with a millimolar extinction coefficient of 3.5 x lo3. It contains 0.45 yo copper which can be completely removed by dialysis against cyanide. The molecular weight is 14,600 (S20, = 1-58 x Dz0, = 10.6 x 10-7). Azurin can be reduced by cysteine, glutathione and other reducing agents ; the blue colour disappears on reduction. Azurin undergoes reduction in the presence of concentrated cell-free extracts of B. bronchiseptica and succinate, and is reoxidized on aeration.

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Section: Methodsmentioning

confidence: 99%

Azurin: A Copper Protein Found in Bordetella

Sutherland

Wilkinson

1963

Journal of General Microbiology

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“…The degradation of toxic amounts of, e.g., aldehydes (91), benzylamines (96), and phenol (19,73) has been found to be stimulated upon addition of montmorillonite or activated carbon. Also, the adsorption of specific inhibitors, and, as a consequence, their removal from the solution have been shown to promote bacterial growth (38,97).…”

Section: Small Moleculesmentioning

confidence: 99%

Influence of interfaces on microbial activity.

Loosdrecht¹,

Lyklema²,

Norde³

et al. 1990

Microbiological Reviews

503

206

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“…Anil~erlite CG-120 chromatograpllic resin, 11 hich promoted growth both in shake flasks and in the fermenter, has a strong negative charge as does glass. Chromatographic resins have been used to remove inhibitors from cultures of Bordetellu pertussis by Sutherland & Wilkinson (1961). That the action of the resin is similar t o the action of a glass surface is suggested by the use of glass beads to remove inhibitors from culture-of the same organism 19%).…”

Section: Stoichiornet Y Of Methane Oxidationmentioning

confidence: 99%

Quantitative Aspects of Growth of the Methane Oxidizing Bacterium Methylococcus capsulatus on Methane in Shake Flask and Continuous Chemostat Culture

Harwood

Pirt

1972

Journal of Applied Bacteriology

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Experiments were directed towards the production of high biomass concentrations in cultures of Methylococcus capaulatus. In shake flasks the effects of ammonium ion, phosphate ion and various trace metals on growth were studied. I n the chemostat the effects on growth of methane limitation, oxygen limitation, aeration, dilution rate, pH value and temperature wore studied and carbon balances were made in steady state conditions. Growth in the fermenter was stimulated by the use of Amberlite CG-120 ion exchange resin in the medium. The probability that Amberlite removed a growth inhibitor is discussed.MANY NEW SPECIES of methane-utilizing bacteria have been isolated in the past few ymrs (Whittenbury, Phillips & Wilkinson, 1970). The possibility of producing single cell protein from natural gas is attractive, but the economics of such a process are speculative because we lack precise information on factors governing growth of these organisms. This lack probably reflects the considerable technical difficulty in growing pure cultures of these bacteria. Our work is concerned with the growth of one species of methylotrophic bacterium, Methylococcus cupsulatus, in shake flasks and in continuous chemostat culture and presents new data on factors influencing the production of biomass and the stoichiometry of methane oxidation. Materials and Methods OrganismThe strain of M . capsulatus isolated by Foster & Davis (1966) was used. Stock suspensions were kindly supplied by Professor J. R. Nonis, Shell Research Limited, Broad Oak Road, Sittingbourne, Kent. HediumThe liquid medium (T4) used both in shake flasks and in continuous culture was made up in 3 parts, A, B

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A new growth medium for virulent Bordetella pertussis

Cited by 24 publications

References 12 publications

Azurin: A Copper Protein Found in Bordetella

Azurin: A Copper Protein Found in Bordetella

Influence of interfaces on microbial activity.

Quantitative Aspects of Growth of the Methane Oxidizing Bacterium Methylococcus capsulatus on Methane in Shake Flask and Continuous Chemostat Culture

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