A new glucomannan from Bletilla striata: Structural and anti-fibrosis effects

Wang, Ying; Liú, Dan; Chen, Shujun; Wang, Yan; Jiang, Huifen; Yin, Huijuan

doi:10.1016/j.fitote.2013.10.008

Cited by 87 publications

(44 citation statements)

References 16 publications

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“…B. striata polysaccharide (BSP) is extracted from B. striata and consists of α-mannose, β-mannose and β-glucose (17). BSP has a number of biological functions, including anti-inflammatory (18), anti-tumor (19), anti-fibrosis (20) and antibacterial (21) properties, as well as inducing endothelial cell proliferation and vascular endothelial growth-factor expression (16). However, to the best of our knowledge, no previous studies have investigated the protective role of BSP in epithelial barrier disruption.…”

Section: Introductionmentioning

confidence: 99%

Bletilla�striata polysaccharide has a protective effect on intestinal epithelial barrier disruption in TAA‑induced cirrhotic rats

et al. 2018

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It has been reported that intestinal epithelial barrier dysfunction serves an important role in the development of liver cirrhosis. However, at present there is no satisfactory treatment for intestinal mucosal lesions and ulcers associated with cirrhosis. The aim of the present study was to investigate the effect of Bletilla striata polysaccharide (BSP) on intestinal epithelial barrier disruption in rats with thioacetamide (TAA)-induced liver cirrhosis. Rats were randomly divided into 5 groups (n=10): BSP low dosage (15 mg/kg), BSP middle dosage (30 mg/kg), BSP high dosage (60 mg/kg), experiment and control groups. All groups except control group were administered with TAA (200 mg/kg/day) to induce liver cirrhosis. Following modeling, rats in the low, middle and high-dose BSP groups received BSP. ELISA kits were used to measure the endotoxin, alanine transaminase (ALT) and aspartate transaminase (AST) levels in the portal vein, while interleukin (IL)-6 and tumor necrosis factor (TNF)-α expression in the ileal tissue was measured. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting were used to detect the expression of zonula occludens (ZO)-1 and occludin mRNA and protein, respectively. Intestinal epithelial tissue pathology was detected using hematoxylin-eosin (HE) staining. Immunohistochemistry was performed to assess the expression of ZO-1 and occludin in intestinal epithelial tissues. Following treatment with BSP, ALT, AST and endotoxin levels in the portal vein, as well as IL-6 and TNF-α expression in ileal tissues, were significantly decreased compared with model group (P<0.05 or 0.01). Furthermore, BSP treatment upregulated the expression of ZO-1 and occludin mRNA and protein compared with the model group (P<0.05 or 0.01) and cytoplasmic staining for these proteins was increased. The degree of intestinal epithelial tissue pathological damage was significantly reduced in the BSP groups. In conclusion, BSP is able to reduce endotoxin levels, inhibit the inflammatory cytokines IL-6 and TNF-α and elevate expression of ZO-1 and occludin at tight junctions. Together, these results suggest a novel protective agent for the restoration of intestinal epithelial barrier disruption.

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Section: Introductionmentioning

confidence: 99%

Bletilla�striata polysaccharide has a protective effect on intestinal epithelial barrier disruption in TAA‑induced cirrhotic rats

et al. 2018

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“…polysaccharides (BSPs), the major active ingredients of B . striata , were extracted from the tubers, and are composed of (1→2)-α-D-mannopyranose and (1→4)-β-D-glucose, as characterized by nuclear magnetic resonance spectroscopy [16]. BSPs have been used in pharmaceutical and biomedical industry because of their negligible cytotoxic effects and properties, such as biocompatibility and biodegradability [17–19].…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo evaluation of docetaxel-loaded stearic acid-modified Bletilla striata polysaccharide copolymer micelles

et al. 2017

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Bletilla striata polysaccharides (BSPs) have been used in pharmaceutical and biomedical industry, the aim of the present study was to explore a BSPs amphiphilic derivative to overcome its application limit as poorly water-soluble drug carriers due to water-soluble polymers. Stearic acid (SA) was selected as a hydrophobic block to modify B. striata polysaccharides (SA-BSPs). Docetaxel (DTX)-loaded SA-BSPs (DTX-SA-BSPs) copolymer micelles were prepared and characterized. The DTX release percentage in vitro and DTX concentration in vivo was carried out by using high performance liquid chromatography. HepG2 and HeLa cells were subjected to MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazonium bromide) assay to evaluate the cell viability. In vitro evaluation of copolymer micelles showed higher drug encapsulation and loading capacity. The release percentage of DTX from DTX-SA-BSPs copolymer micelles and docetaxel injection was 66.93 ± 1.79% and 97.06 ± 1.56% in 2 days, respectively. The DTX-SA-BSPs copolymer micelles exhibited a sustained release of DTX. A 50% increase in growth inhibition was observed for HepG2 cells treated with DTX-SA-BSPs copolymer micelles as compared to those treated with docetaxel injection for 72 h. DTX-SA-BSPs copolymer micelles presented a similar growth inhibition effect on Hela cells. Furthermore, absolute bioavailability of DTX-SA-BSPs copolymer micelles was shown to be 1.39-fold higher than that of docetaxel injection. Therefore, SA-BSPs copolymer micelles may be used as potential biocompatible polymers for cancer chemotherapy.

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“…Chinese pharmacopeia (2010) recorded that B. striata has functions of hemostasis, detumescence and bodily function enhancement. Wang, Liu et al (2014) showed that B. striata polysaccharide is composed of (1 → 2)-␣-d-mannopyranose and (1 → 4)-␤-d-glucopyranose residues with using 1D and 2D NMR spectroscopy. At present, studies of polysaccharides from B. striata mainly involve regarding it as a biomaterial in the field of medical applications (Zhang, Zhang, Shi, & Sun, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Diao et al (2008) clarified the mechanism of the polysaccharide from B. striata on wound healing. Wang, Liu et al (2014) noted that the B. striata polysaccharide was pivotal in protection against the effects of renal fibrosis, which is probably mediated by downregulated TGF-␤ RI, TGF-␤ RII and ␣-SMA in vitro. In addition, some studies present that the B. striata polysaccharide can be used as an additive in the food and cosmetics industries (Liu, Huang, Huang, & Wang, 2005 The polysaccharide has a wide variety of functions because of its particular complex structure and certain molecular weight.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical characterization of the polysaccharide from Bletilla striata : Effect of drying method

Kong

Feng

et al. 2015

Carbohydrate Polymers

189

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A new glucomannan from Bletilla striata: Structural and anti-fibrosis effects

Cited by 87 publications

References 16 publications

Bletilla�striata polysaccharide has a protective effect on intestinal epithelial barrier disruption in TAA‑induced cirrhotic rats

Bletilla�striata polysaccharide has a protective effect on intestinal epithelial barrier disruption in TAA‑induced cirrhotic rats

In vitro and in vivo evaluation of docetaxel-loaded stearic acid-modified Bletilla striata polysaccharide copolymer micelles

Physicochemical characterization of the polysaccharide from Bletilla striata : Effect of drying method

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