A new glanapterygine catfish of the genus Listrura (Siluriformes: Trichomycteridae) from southeastern Brazil, corroborated by morphological and molecular data

Abstract

Listrura costai, new species, is described from small streams in a swampy coastal plain in the rio Jurumirim basin, Angra dos Reis Municipality, Rio de Janeiro State, southeastern Brazil. The new species is morphologically very similar to L. nematopteryx and L. picinguabae, all possessing only one long pectoral-fin ray. It differs from its congeners by possessing an autapomorphic character: first hypobranchial with an anterior process (vs. process absent). Other features such as coloration, numbers of opercular and interopercular odontodes, number of anal-fin rays, head length, and shape of some bone structures help to distinguish the new species from L. nematopteryx and L. picinguabae. Molecular analyses using partial sequences of the mitochondrial DNA genes cytochrome oxidase c subunit 1 and cytochrome b from the new species and morphologically similar species are provided. The results about both molecular markers corroborate the validity of the new species by significant genetic distance values between it and congeneric species, and by its phylogenetic position in the hypotheses performed by maximum-parsimony method.Listrura costai, espécie nova, é descrita de exemplares obtidos em pequenos córregos restritos à bacia do rio Jurumirim, município de Angra dos Reis, estado do Rio de Janeiro, sudeste do Brasil. A espécie nova é morfologicamente muito similar a L. nematopteryx e L. picinguabae, todas possuindo um único e longo raio na nadadeira peitoral. Difere-se dos seus congêneres por possuir um caráter autapomórfico: primeiro hipobranquial com um processo anterior (vs. processo ausente). Outras características como coloração, número de odontóides operculares e interoperculares, número de raios na nadadeira anal, e forma de algumas estruturas ósseas ajudam a diferenciar a nova espécie de L. nematopteryx e L. picinguabae. Análises moleculares usando sequências parciais dos genes mitocondriais citocromo oxidase c subunidade 1 e citocromo b da nova espécie e espécies morfologicamente similares são apresentadas. Os resultados de ambos os marcadores moleculares corroboram a validade da espécie nova pelos valores significativos das distâncias genéticas entre esta e as espécies congenéricas, e por sua posição filogenética nas hipóteses feitas pelo o método de máxima parcimônia.

A new species of Ituglanis (Siluriformes: Trichomycteridae) from the rio Uruguai basin, southern Brazil

“…2). A similar process was observed in representatives of the basal trichomycterid subfamilies Copionodontinae and Trichogeninae, in two Trichomycterus species -T. pardus Cope (= T. rivulatus Valenciennes) and T. spelaeus DoNascimiento, Villarreal & Provenzano -and in the glanapterygine Listrura costai VillaVerde, Lazzarotto & Lima (de Pinna, 1998;DoNascimiento et al, 2001;Wosiacki, 2002;Villa-Verde et al, 2012). In Ituglanis, a similar process was only observed at the right side of a single paratype of I. goya (F. Langeani, PhD: written communication, March 2017).…”

Multigene phylogeny reveals convergent evolution in small interstitial catfishes from the Amazon and Atlantic forests (Siluriformes: Trichomycteridae)

“…The analyses included a set of partial sequences of two nuclear genes: recombination activating 2 (RAG2) and myosin heavy chain 6 (MYH6); and partial sequences of three mitochondrial genes: 16S ribosomal RNA (16S), cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit 4 (ND4). Amplification of the target DNA fragments was made through the polymerase chain reaction (PCR) method, using the following primers: MHRAG2‐F1 and MHRAG2‐R1 (Hardman & Page, ), RAG2 TRICHO F (5′‐GACAGYCGAGGATGCAATCGGAA‐3′) and RAG2 TRICHO R (5′‐CTGTCCTSCATYTCATGGGGTTCACG‐3′), herein described; myh6_F459 and myh6_R1322 (Li, Ortí, Zhang, & Lu, ), MYH6 TRICHO F (5′‐ACK GAC AGA GAG AAC CAG TC‐3′) and MYH6 TRICHO R (5′‐ACK GAC AGA GAG AAC CAG TC‐3′), herein described; 16SarL and 16SbrH (Palumbi, Martin, Romano, Stice, & Grabowski, ); L5698‐ASN and H7271‐COI (Villa‐Verde et al, ), FISHF1 and FISHR1 (Ward, Zemlak, Innes, Last, & Hebert, ); ND4 H3 L11935 and H12857 (Palumbi et al, ). Double‐stranded PCR amplifications were performed in 60 μl reactions with reagents at the following concentrations: 5 × GreenGoTaq Reaction Buffer (Promega), 3.2 mm MgCl 2 , 1 μm of each primer, 75 ng of total genomic DNA, 0.2 mm of each dNTP and 1 U of standard Taq polymerase or Promega GoTaq Hot Start polymerase.…”

A new species of Hyphessobrycon Durbin from northeastern Brazil: evidence from morphological data and DNA barcoding (Characiformes, Characidae)

“…The most widespread molecular method used in taxonomy has been the DNA barcoding, which consists on the use of a single gene from mitochondrial DNA (cytochrome oxidase subunit I – COI) as a proxy for species differentiation (Hebert et al 2003). In fact, several studies have been carried out using molecular markers and new species have been delimited and/or described, in most cases, based both on molecular and morphological evidence (e.g., Costa and Amorim 2011, Costa et al 2012, Roxo et al 2012, Villa-Verde et al 2012, Castro-Paz et al 2014, Costa et al 2014, Benzaquem et al 2015, Mattos et al 2015, Costa et al 2017). …”