A new genomic species in Borrelia burgdorferi sensu lato isolated from Japanese ticks

Postić, D.; Belfaiza, J; Isogai, Emiko; Girons, Isabelle Saint; Grimont, Patrick A. D.; Baranton, Guy

doi:10.1016/0923-2508(93)90054-6

Cited by 58 publications

(41 citation statements)

References 22 publications

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“…The Borrelia species identity as determined by antigenic differences has been suggested to correlate with the origin of ticks. In Japan, isolates from I. ovatus were different from those of I. persulcatus (8,24,25,33). In our survey, it may be suggested that I. ovatus and H. flava can acquire spirochetes by feeding on a same deer affected with I. persulcatus.…”

Section: Discussionmentioning

confidence: 81%

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Borrelia burgdorferi Sensu Lato in an Endemic Environment: Wild Sika Deer (Cervus nippon yesoensis) with Infected Ticks and Antibodies

Isogai

Masuzawa

et al. 1996

Microbiology and Immunology

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Ticks and blood samples were collected from wild sika deer (Cervus nippon yesoensis) during a hunting season (August to October) of 1991 at a selected location in Hokkaido, Japan. Ixodes persulcatus (adult and nymph) and I. ovatus (adult) were the common ticks on sika deer. Spirochetes were detected in the midgut of the ticks by the indirect peroxidase-conjugated antibody staining method and by dark-field microscopy after cultivation. By the reactive pattern of monoclonal antibodies, isolates were considered to belong to Borrelia garinii or B. japonica. In an antibody test, the percentage of seropositive deer was 69.0%. Most of the adult sika deer were positive for antibodies to the spirochetes. There are significant age-dependency in antibody level and seropositive rate. The surveillance of deer should be valuable in monitoring the transmission risk of B. burgdorferi sensu lato in nature.

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Section: Discussionmentioning

confidence: 81%

“…However, there have been reported suspected cases on the basis of serological tests (19,26). B. japonica found in I. ovatus was a new species (24,33). In our experiments, several inbred mouse strains were infected with B. japonica but the initial subcutaneous lesions were mild (unpublished data).…”

Section: Discussionmentioning

confidence: 95%

Borrelia burgdorferi Sensu Lato in an Endemic Environment: Wild Sika Deer (Cervus nippon yesoensis) with Infected Ticks and Antibodies

Isogai

Masuzawa

et al. 1996

Microbiology and Immunology

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“…Members of the species complex are distributed throughout the temperate zones of the Northern Hemisphere. Initially, most species of the genus Borrelia within the complex were delineated using DNA-DNA hybridization or diversity at the rrs locus (Baranton et al, 1992;Fukunaga et al, 1996;Le Fleche et al, 1997;Masuzawa et al, 2001;Postic et al, 1998Postic et al, , 1993. However, more recently multilocus sequence analysis (MLSA) has been accepted as a taxonomic instrument for bacterial species assignment (Bishop et al, 2009;Gevers et al, 2005) and was used to delineate species of the genus Borrelia (Postic et al, 2007;Richter et al, 2006;Rudenko et al, 2011).…”

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confidence: 99%

Borrelia bavariensis sp. nov. is widely distributed in Europe and Asia

Margos

Wilske²,

Sing³

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

105

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Since the original description of Borrelia bavariensis sp. nov. in 2009, additional samples available from humans and ticks from Europe and Mongolia, respectively, have been used to further characterize Borrelia strains belonging to this group of spirochaetes that utilize rodents as reservoir hosts. These investigations suggested the presence of related strains in Europe and Asia and confirmed their status as representing a distinct species. Furthermore, samples that were investigated by researchers from China and Japan confirm the ecological relationship of members of this proposed species with rodents and suggest that it has a wide distribution in Eurasia. Here, we use phylogenetic and genetic distance analyses to validate B. bavariensis sp. nov. as a species within the Borrelia burgdorferi sensu lato species complex. The type strain is PBiT ( = DSM 23469T = BAA-2496T).

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“…Borrelia burgdorferi sensu lato are classified into seven species: B. burgdorferi (7,13) sensu stricto isolated in North America and Europe; Borrelia garinii (3) and Borrelia afzelii (8) isolated in Europe and Japan; Borrelia japonica (14,25), Borrelia tanukii (10,17,19) and Borrelia trudae (10,17) isolated from Ixodes ovatus, Ixodes tanuki and Ixodes turdus in Japan; and Borrelia andersonii isolated from Ixodes dentatus in North America (15). Postic et al (24) developed a simple and rapid method for identification of species of B. burgdorferi sensu lato based on restriction fragment length polymorphism (RFLP) analysis of the 5S-23S ribosomal RNA (rRNA) intergenic spacer amplicon, and identified three genomic groups (Groups DN127, VS116 and Poti B2) among isolates from North America and Europe.…”

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confidence: 99%

Genetic Diversity of Borrelia burgdorferi Sensu Lato Isolated in Far Eastern Russia

Masuzawa

Iwaki

Sato

et al. 1997

Microbiology and Immunology

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One-hundred and fifty-seven Borrelia isolated from adult ticks, Ixodes persulcatus, and wild rodents, Clethrionomys rufocanus and Apodemus peninsulae, in the far eastern part of Russia were charaeterized and identified by restriction fragment length polymorphism (RFLP) of the 5S-23S rRNA intergenic spacer. Some isolates showed unique RFLP patterns and were determined as Borrelia garinii on the basis of a sequence analysis of the intergenic spacer amplicon and reactivity with species-specific monoclonal antibodies (MAbs). 86.5 and 12.7% of the tick isolates, and 74.2 and 12.9% of the rodent isolates were determined as Borrelia garinii and Borrelia afzelii, respectively, but no Borrelia burgdorferi sensu stricto was detected. This finding is similar to the results obtained from Borrelia surveys of I. persulcatus and wild rodents in Hokkaido, Japan.

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A new genomic species in Borrelia burgdorferi sensu lato isolated from Japanese ticks

Cited by 58 publications

References 22 publications

Borrelia burgdorferi Sensu Lato in an Endemic Environment: Wild Sika Deer (Cervus nippon yesoensis) with Infected Ticks and Antibodies

Borrelia burgdorferi Sensu Lato in an Endemic Environment: Wild Sika Deer (Cervus nippon yesoensis) with Infected Ticks and Antibodies

Borrelia bavariensis sp. nov. is widely distributed in Europe and Asia

Genetic Diversity of Borrelia burgdorferi Sensu Lato Isolated in Far Eastern Russia

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