A new gene from <i>Xenorhabdus bovienii</i> and its encoded protease inhibitor protein against <i>Acyrthosiphon pisum</i>

Zeng, Fanrong; Xue, Renfeng; Zhang, Heqing; Jiang, Taozhen

doi:10.1002/ps.3299

Cited by 11 publications

(14 citation statements)

References 44 publications

(53 reference statements)

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“…The gene encoding the protease inhibitor protein has been recognized and expressed in the symbiotic bacterium Xenorhabdus b ovienii strains BJFS526 and Xbpi-1. This protein’s impact on the pea aphid Acyrthosiphon pisum was also investigated ( Zeng et al, 2012 ; Jin et al, 2014 ). Xenorhabdus szentirmaii is a one-of-a-kind source of antimicrobial peptides that are effective against virtually all known phytopathogens ( Fuchs et al, 2014 ; Fodor et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)

et al. 2022

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The current work is aimed at isolating and identifying new Entomopathogenic bacterium (EPB) strains associated with Steinernema feltiae and assessing the EPB’s biocontrol potential on Aphis punicae and Aphis illinoisensis adults in the laboratory. From S. feltiae, five bacterial isolates were isolated and molecularly characterized. Lysinibacillus xylanilyticus strain TU-2, Lysinibacillus xylanilyticus strain BN-13, Serratia liquefaciens strain TU-6, Stenotrophomonas tumulicola strain T5916-2-1b, and Pseudochrobactrum saccharolyticum strain CCUG are the strains. Pathogenicity tests demonstrated that bacterial cells were more toxic against the two aphid species than bacterial cell-free supernatants. S. tumulicola strain T5916-2-1b cells and filtrate were reported to have the strongest potential to kill A. punicae and A. illinoisensis individuals within 6 h after treatment, with 100% mortality of both insects 24 and 48 h after treatment. Based on the results of the study, it looked like endogenous Steinernema-associated EPB could be used directly as a biocontrol agent for A. punicae and A. illinoisensis.

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Section: Introductionmentioning

confidence: 99%

Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)

et al. 2022

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“…The protease inhibitor protein encoded gene from strains BJFS526 and Xbpi-1 of the symbiotic bacterium Xenorhabdus bovienii has been identified and expressed. The effects of this protein on pea aphids ( Acyrthosiphon pisum ) have also been investigated [ 16 , 17 ]. Fuchs et al [ 18 ] reported that Xenorhabdus szentirmaii is a unique source of highly efficient antimicrobial peptides against almost all known plant pathogens.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification, and Biocontrol Potential of Entomopathogenic Nematodes and Associated Bacteria against Virachola livia (Lepidoptera: Lycaenidae) and Ectomyelois ceratoniae (Lepidoptera: Pyralidae)

Alotaibi

Darwish

Zaynab

et al. 2022

Biology

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Virachola livia (Lepidoptera: Lycaenidae) and Ectomyelois ceratoniae (Lepidoptera: Pyralidae) are the key pests of pomegranates in Saudi Arabia that are managed mainly using broad-spectrum pesticides. Interactions between entomopathogenic nematodes (EPNs), Steinernematids, Heterorhabditids, and their entomopathogenic bacterial symbionts (EPBs) have long been considered monoxenic 2-partner associations responsible for killing insects and, therefore, are widely used in insect pest biocontrol. However, there are limited reports identifying such organisms in Taif, Saudi Arabia. The current study aimed to identify the EPNs and their associated bacteria isolated from Taif, Saudi Arabia, and evaluate their biocontrol potential on third instars of V. livia and E. ceratoniae larvae under laboratory conditions. A total of 35 EPN isolates belonging to Steinernema (20) and Heterorhabditis (15) were recovered from 320 soil samples. Twenty-six isolates of symbiotic or associated bacteria were isolated from EPNs and molecularly identified as Xenorhabdus (6 isolates), Photorhabdus (4 isolates), Pseudomonas (7), or Stenotrophomonas (9). A pathogenicity assay revealed that Steinernema spp. were more virulent than Heterorhabditis spp. against the two pomegranate insects, with LC50 values of 18.5 and 13.6 infective juveniles (IJs)/larva for Steinernema spp. and 52 and 32.4 IJs/larva for Heterorhabditis spp. at 48 and 72 h post-treatment of V. livia larvae, respectively. Moreover, LC50 values of 9 and 6.6 IJs/larva (Steinernema spp.) and 34.4 and 26.6 IJs/larva (Heterorhabditis spp.) were recorded for E. ceratoniae larvae at 48 and 72 h post-treatment. Moreover, the EPB Stenotrophomonas maltophilia CQ1, isolated from Steinernema spp., surpassed Pseudomonas mosselii SJ10, associated with Heterorhabditis spp., in their ability to kill V. livia or E. ceratoniae larvae within 6 h post-application, resulting in 100% mortality in both insects after 24 and 48 h of exposure. We conclude that applications of either the endogenous EPNs’ IJs or their associated EPB cells could serve as potential biocontrol agents for V. livia and E. ceratoniae.

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“…On the other hand, analysis of the 16S rRNA gene allowed for the identi cation of X. bovienii, the symbiotic bacterium of S. feltiae [6]. This bacterium species, like other members of the genus, produce a toxin complex with a potential use in crop protection against different insect pests [4,43].…”

Section: Discussionmentioning

confidence: 99%

Morphological, molecular and ecological characterization of a native isolate of Steinernema feltiae (Rhabditida: Steinernematidae) from southern Chile

Flores

Alvarado

Lankin

et al. 2020

Preprint

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Background: Steinernema feltiae is an entomopathogenic nematode used in biological control programs with a global distribution. Populations of this species show phenotypic plasticity derived from local adaptation, and vary in different traits, such as location and host penetration. The aim of this work was to describe a Chilean isolate of this nematode species, using integrative approaches.Methods: Nematode morphological and morphometric studies were conducted along with molecular analysis of nuclear genes. The symbiotic bacterium was also identified by sequencing the 16S rRNA gene. Some ecological characteristics were described, including the temperature requirements for nematode life cycle and the effect of soil water content for optimal reproduction. Results: Morphometric characterization revealed a large intra-specific variability. The isolate identity was also corroborated with the analysis of nuclear genes. Based on the 16S gene, its symbiont bacteria, Xenorhabdus bovienii, was identified. The lowest, optimal and highest temperatures found to limit the infestation and reproduction on Galleria mellonella were 10, 20 and 30 °C respectively; the emergence from the host larvae occurred approximately ten days after inoculation. Differences were observed in offspring, and 120 infective juveniles (IJ)/larva was the most prolific dose at 20 °C. The soil water content did not affect the number of IJ invaders, penetration efficacy and IJ emergence time or offspring per larva, but it caused a delay in achieving full mortality at the permanent wilting point with respect to saturation and field capacity.Conclusions: For the first time, a Chilean isolate of S. feltiae is described in detail considering morphological, molecular and ecological aspects. The isolate showed to be efficient in soil containing water, with optimal temperatures ranging from 15 to 25 °C for host infestation and production of an abundant offspring; these characteristics would allow its potential use as control agents in a wide geographical area of the country

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A new gene from Xenorhabdus bovienii and its encoded protease inhibitor protein against Acyrthosiphon pisum

Abstract: The results from this study suggest that this gene and the protease inhibitor protein encoded may offer an alternative method to control aphids in the future.

Cited by 11 publications

References 44 publications

Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)

Identification and environment-friendly biocontrol potential of five different bacteria against Aphis punicae and Aphis illinoisensis (Hemiptera: Aphididae)

Isolation, Identification, and Biocontrol Potential of Entomopathogenic Nematodes and Associated Bacteria against Virachola livia (Lepidoptera: Lycaenidae) and Ectomyelois ceratoniae (Lepidoptera: Pyralidae)

Morphological, molecular and ecological characterization of a native isolate of Steinernema feltiae (Rhabditida: Steinernematidae) from southern Chile

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