A new G-triplex-based strategy for sensitivity enhancement of the detection of endonuclease activity and inhibition

Gao, Congcong; Che, Baoquan; Dai, Hong

doi:10.1039/d1ra04203c

Cited by 3 publications

(1 citation statement)

References 44 publications

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“…Nevertheless, G-quadruplexes are problematic in terms of releasing and controlling their structure, when applied to biochemical analysis and detection [33]. As folding intermediates of G-quadruplexes, G-triplexes have shorter base sequences and novel DNA structures [34]. Therefore, the design of SS-aptamers based on G-triplexes is easier than the design of those based on G-quadruplexes.…”

Section: Feasibility Analysis Of Structure-switching Aptasensorsmentioning

confidence: 99%

Structure‐switching aptasensors for sensitive detection of ochratoxin A

Fan

Jiang

et al. 2023

Luminescence

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Ochratoxin A (OTA) is a toxic metabolite commonly found in various foods and feedstuffs. Accurate and sensitive detection of OTA is needed for food safety and human health. Based on a common OTA‐binding aptamer (OTABA), two structure‐switching OTABAs, namely OTABA4 and OTABA3, were designed by configuring a split G‐quadruplex and a split G‐triplex, respectively, at the two ends of OTABA to construct aptasensors for the detection of OTA. The OTABA, G‐quadruplex, and G‐triplex all can capture the thioflavin T (ThT) probe, thereby enhancing the fluorescence intensity of ThT. Bonding with OTA could change the conformations of OTABA and G‐quadruplex or G‐triplex regions, resulting in the release of the captured ThT and diminution of its fluorescence intensity. Dual conformation changes in structure‐switching OTABA synergistically amplified the fluorescence signal and improved the sensitivity of the aptasensor, especially for that with OTABA3. The detection limits of the OTABA4‐ThT and OTABA3‐ThT systems for OTA were 0.28 and 0.059 ng ml−1, with a 1.4‐fold and 6.7‐fold higher sensitivity than that of the original OTABA‐ThT system, respectively. They performed well in corn and peanut samples and met the requirements of the food safety inspections.

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Section: Feasibility Analysis Of Structure-switching Aptasensorsmentioning

confidence: 99%

Structure‐switching aptasensors for sensitive detection of ochratoxin A

Fan

Jiang

et al. 2023

Luminescence

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Determination of Endonuclease Activity by an Enzyme-Free Fluorescent Biosensor Using the Hybridization Chain Reaction (HCR)

Lin

Wang

et al. 2022

Analytical Letters

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Calcium-Dependent Chemiluminescence Catalyzed by a Truncated c-MYC Promoter G-Triplex DNA

Das,

Williams,

Myhre

et al. 2024

Molecules

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The dynamic landscape of non-canonical DNA G-quadruplex (G4) folding into G-triplex intermediates has led to the study of G-triplex structures and their ability to serve as peroxidase-mimetic DNAzymes. Here we report the formation, stability, and catalytic activity of a 5′-truncated c-MYC promoter region G-triplex, c-MYC-G3. Through circular dichroism, we demonstrated that c-MYC-G3 adopts a stable, parallel-stranded G-triplex conformation. The chemiluminescent oxidation of luminol by the peroxidase mimicking DNAzyme activity of c-MYC-G3 was increased in the presence of Ca2+ ions. We utilized surface plasmon resonance to characterize both c-MYC-G3 G-triplex formation and its interaction with hemin. The detailed study of c-MYC-G3 and its ability to form a G-triplex structure and its DNAzyme activity identifies issues that can be addressed in future G-triplex DNAzyme designs.

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A new G-triplex-based strategy for sensitivity enhancement of the detection of endonuclease activity and inhibition

Abstract: A new G-triplex-based probe was developed for detecting EcoRI activity and inhibition. The probe showed good selectivity towards EcoRI. The assay was colorimetric and can be monitored by the naked eye.

Cited by 3 publications

References 44 publications

Structure‐switching aptasensors for sensitive detection of ochratoxin A

Structure‐switching aptasensors for sensitive detection of ochratoxin A

Determination of Endonuclease Activity by an Enzyme-Free Fluorescent Biosensor Using the Hybridization Chain Reaction (HCR)

Calcium-Dependent Chemiluminescence Catalyzed by a Truncated c-MYC Promoter G-Triplex DNA

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