A New Function of MbIAA19 Identified to Modulate Malus Plants Dwarfing Growth

Wang, Jian; Xue, Li; Zhang, Xiao; Hou, Yali; Zheng, Ke; Fu, Dongxu; Dong, Wenxuan

doi:10.3390/plants12173097

Cited by 4 publications

(1 citation statement)

References 69 publications

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“…To explore the molecular mechanisms of auxin signal transduction, Aux/IAA and ARF genes have been identified and characterized in several plant species, including Arabidopsis thaliana [ 13 ], Malus [ 14 ], Zea mays [ 15 ], Coix lacryma-jobi [ 16 ], tomato [ 17 ], soybean [ 18 ], Vitis vinifera [ 19 ], Brassica rapa [ 20 ], Triticum aestivum [ 21 ], sorghum [ 22 ], Citrus sinensis [ 23 ] and Cucumis sativus [ 24 ]. Previous studies have found that the functions of Aux/IAA and ARF genes are relatively different in different plants [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of PmaIAA27 and PmaARF15 genes on drought stress tolerance in pinus massoniana

Li,

Quan

et al. 2023

BMC Plant Biol

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Background Auxin plays an important role in plant resistance to abiotic stress. The modulation of gene expression by Auxin response factors (ARFs) and the inhibition of auxin/indole-3-acetic acid (Aux/IAA) proteins play crucial regulatory roles in plant auxin signal transduction. However, whether the stress resistance of Masson pine (Pinus massoniana), as a representative pioneer species, is related to Aux/IAA and ARF genes has not been thoroughly studied and explored. Results The present study provides preliminary evidence for the regulatory role of the PmaIAA27 gene in abiotic stress response in Masson pine. We investigated the effects of drought and hormone treatments on Masson pine by examining the expression patterns of PmaIAA27 and PmaARF15 genes. Subsequently, we conducted gene cloning, functional testing using transgenic tobacco, and explored gene interactions. Exogenous auxin irrigation significantly downregulated the expression of PmaIAA27 while upregulating PmaARF15 in Masson pine seedlings. Moreover, transgenic tobacco with the PmaIAA27 gene exhibited a significant decrease in auxin content compared to control plants, accompanied by an increase in proline content - a known indicator of plant drought resistance. These findings suggest that overexpression of the PmaIAA27 gene may enhance drought resistance in Masson pine. To further investigate the interaction between PmaIAA27 and PmaARF15 genes, we performed bioinformatics analysis and yeast two-hybrid experiments which revealed interactions between PB1 structural region of PmaARF15 and PmaIAA27. Conclusion The present study provides new insights into the regulatory functions of Aux/IAA and ARF genes in Masson pine. Overexpression of PmaIAA gene may have negative effects on the growth of Masson pine, but may improve the drought resistance. Therefore, this study has great application prospects.

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Section: Introductionmentioning

confidence: 99%

Effects of PmaIAA27 and PmaARF15 genes on drought stress tolerance in pinus massoniana

Li,

Quan

et al. 2023

BMC Plant Biol

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Target Detection for Coloring and Ripening Potted Dwarf Apple Fruits Based on Improved YOLOv7-RSES

Ma,

Li,

Zhang

et al. 2024

Applied Sciences

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Dwarf apple is one of the most important forms of garden economy, which has become a new engine for rural revitalization. The effective detection of coloring and ripening apples in complex environments is important for the sustainable development of smart agricultural operations. Addressing the issues of low detection efficiency in the greenhouse and the challenges associated with deploying complex target detection algorithms on low-cost equipment, we propose an enhanced lightweight model rooted in YOLOv7. Firstly, we enhance the model training performance by incorporating the Squeeze-and-Excite attention mechanism, which can enhance feature extraction capability. Then, an SCYLLA-IoU (SIoU) loss function is introduced to improve the ability of extracting occluded objects in complex environments. Finally, the model was simplified by introducing depthwise separable convolution and adding a ghost module after up-sampling layers. The improved YOLOv7 model has the highest AP value, which is 10.00%, 5.61%, and 6.00% higher compared to YOLOv5, YOLOv7, and YOLOX, respectively. The improved YOLOv7 model has an MAP value of 95.65%, which provides higher apple detection accuracy compared to other detection models and is suitable for potted dwarf anvil apple identification and detection.

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Morphological Structure Identification, Comparative Mitochondrial Genomics and Population Genetic Analysis toward Exploring Interspecific Variations and Phylogenetic Implications of Malus baccata ‘ZA’ and Other Species

Wang,

Zhang

et al. 2024

Biomolecules

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Malus baccata, a valuable germplasm resource in the genus Malus, is indigenous to China and widely distributed. However, little is known about the lineage composition and genetic basis of ‘ZA’, a mutant type of M. baccata. In this study, we compared the differences between ‘ZA’ and wild type from the perspective of morphology and ultrastructure and analyzed their chloroplast pigment content based on biochemical methods. Further, the complete mitogenome of M. baccata ‘ZA’ was assembled and obtained by next-generation sequencing. Subsequently, its molecular characteristics were analyzed using Geneious, MISA-web, and CodonW toolkits. Furthermore, by examining 106 Malus germplasms and 42 Rosaceae species, we deduced and elucidated the evolutionary position of M. baccata ‘ZA’, as well as interspecific variations among different individuals. In comparison, the total length of the ‘ZA’ mitogenome (GC content: 45.4%) is 374,023 bp, which is approximately 2.33 times larger than the size (160,202 bp) of the plastome (GC: 36.5%). The collinear analysis results revealed abundant repeats and genome rearrangements occurring between different Malus species. Additionally, we identified 14 plastid-driven fragment transfer events. A total of 54 genes have been annotated in the ‘ZA’ mitogenome, including 35 protein-coding genes, 16 tRNAs, and three rRNAs. By calculating nucleotide polymorphisms and selection pressure for 24 shared core mitochondrial CDSs from 42 Rosaceae species (including ‘ZA’), we observed that the nad3 gene exhibited minimal variation, while nad4L appeared to be evolving rapidly. Population genetics analysis detected a total of 1578 high-quality variants (1424 SNPs, 60 insertions, and 94 deletions; variation rate: 1/237) among samples from 106 Malus individuals. Furthermore, by constructing phylogenetic trees based on both Malus and Rosaceae taxa datasets, it was preliminarily demonstrated that ‘ZA’ is closely related to M. baccata, M. sieversii, and other proximate species in terms of evolution. The sequencing data obtained in this study, along with our findings, contribute to expanding the mitogenomic resources available for Rosaceae research. They also hold reference significance for molecular identification studies as well as conservation and breeding efforts focused on excellent germplasms.

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A New Function of MbIAA19 Identified to Modulate Malus Plants Dwarfing Growth

Cited by 4 publications

References 69 publications

Effects of PmaIAA27 and PmaARF15 genes on drought stress tolerance in pinus massoniana

Effects of PmaIAA27 and PmaARF15 genes on drought stress tolerance in pinus massoniana

Target Detection for Coloring and Ripening Potted Dwarf Apple Fruits Based on Improved YOLOv7-RSES

Morphological Structure Identification, Comparative Mitochondrial Genomics and Population Genetic Analysis toward Exploring Interspecific Variations and Phylogenetic Implications of Malus baccata ‘ZA’ and Other Species

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