A new FTIR-ATR cell for drug diffusion studies

Hartmann, Marcus D.; Hanh, Bui Duc; Podhaisky, Helmut; Wensch, Jörg; Bodzenta, Jerzy; Wartewig, S.; Neubert, Reinhard H.H.

doi:10.1039/b408940p

Cited by 19 publications

(10 citation statements)

References 6 publications

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“…Wartewig and coworkers modified the geometry of such measurements to incorporate an acceptor layer between the donor (i.e., drug formulation) and membrane to be studied. 35,36 A number of examples of this approach are given in a review by Wartewig and Neubert, 37 and the reader is advised to examine this article and the references therein for more detail. Kazarian et al 38 used ATR/FT-IR imaging to study the hydration and dissolution kinetics of a number of hydroxy propyl methyl cellulose (HPMC)-based binary pharmaceutical formulations.…”

Section: Volatile Componentmentioning

confidence: 98%

The Role and Potential of Vibrational Spectroscopy in the Study and Characterization of Traditional and Novel Food Packaging Structures

Lagarón

López‐Rubio

2001

Handbook of Vibrational Spectroscopy

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The suitability and potential of vibrational spectroscopic techniques, in particular mid‐infrared spectroscopy, to study molecular organization and structural changes in packaging materials are discussed in this chapter. Specifically, some examples on the use of these techniques to characterize polymer blends, molecular order, and degradation or crosslinking in polymers are given. Moreover, the interest of using vibrational spectroscopy to study packaging materials also relies on its ability to follow mass transport and migration properties, which is of outstanding importance for food‐packaging applications. Finally, the interest of using vibrational spectroscopy techniques in evaluating novel active packaging applications is highlighted, as these techniques can provide information about the mechanisms of the action of, for instance, antimicrobial polymers, and can also be useful in following the release kinetics of bioactive or functional substances from encapsulating polymeric structures.

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Section: Volatile Componentmentioning

confidence: 98%

The Role and Potential of Vibrational Spectroscopy in the Study and Characterization of Traditional and Novel Food Packaging Structures

Lagarón

López‐Rubio

2001

Handbook of Vibrational Spectroscopy

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“…9,10 It is also able to measure humidity variations 11 and the structure of lipid bilayers. 12 In this context, Fourier transform infrared ͑FTIR͒ spectroscopic imaging was applied to skin to measure the heterogeneity of the skin at a microscopic level.…”

Section: Introductionmentioning

confidence: 99%

In situ permeation study of drug through the stratum corneum using attenuated total reflection Fourier transform infrared spectroscopic imaging

2009

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Infrared (IR) spectroscopy is one of the most chemically specific analytical methods that gives information about composition, structure, and interactions in a material. IR spectroscopy has been successfully applied to study the permeation of xenobiotics through the skin. Combining IR spectroscopy with an IR array detector led to the development of Fourier transform infrared (FTIR) spectroscopic imaging, which generates chemical information from different areas of a sample at the microscopic level. This is particularly important for heterogeneous samples, such as skin. Attenuated total reflectance [corrected] (ATR)-FTIR imaging has been applied to measure, in situ, the diffusion of benzyl nicotinate (BN) through the outer layer of human skin [stratum corneum (SC)]. In vitro experiments have demonstrated the heterogeneous distribution of SC surface lipids before the penetration of a saturated solution of BN. Image analysis demonstrated a strong correlation between the distribution of lipids and drugs, while ethanol appeared to be homogenously distributed in the SC. These results show the ability of ATR-FTIR imaging to measure simultaneously the affinities of drug and solvent to the lipid-rich and lipid-poor skin domains, respectively, during permeation. This information may be useful in better understanding drug-diffusion pathways through the SC.

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“…The permeation experiments were conducted with the IFS 28 spectrometer and an ATR measuring unit from Thermo Spectra Tech with the Teflon diffusion cell developed by Hartmann et al [32] and modified by Günther [33] (Fig. 2).…”

Section: Methodsmentioning

confidence: 99%

Dermal Peptide Delivery Using Enhancer Molecules and Colloidal Carrier Systems – Part IV: Search for an Alternative Model Membrane for Future ATR Permeation Studies Using PKEK as the Model Substance

Ac¹,

Rhh

2019

Skin Pharmacol Physiol

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The main barrier of the human skin is the stratum corneum (SC). Its properties (also depending on the health and age of the individual) and its influence on improved penetration of active ingredients into the skin are the subject of many research projects. Since the availability of human skin, as the ideal model membrane, is limited, the aim of this study was to find a suitable alternative model membrane from the animal kingdom. The alternative model membrane should be used in subsequent permeation experiments with the Teflon diffusion cell instead of human SC. Previous studies have already investigated the permeation properties of pig, snake, and human skin, but not in a Teflon diffusion cell using ATR. Therefore, it first had to be proven that comparable results can be achieved with animal membranes even under these measurement conditions. This is the precondition for meaningful future permeation experiments with potential enhancers. For this purpose, permeation experiments on various model membranes (human isolated SC, sunburned SC, pig isolated SC, and shed snake skin) by means of FTIR-ATR in a Teflon diffusion cell containing the acceptor and the donor compartment as well as the model membrane were conducted and concentration-time courses of the model peptide PKEK determined. These concentration-time courses were used to calculate and compare the pharmacokinetic parameters (permeation coefficients, lag time, and flux). The starting point was a 10% PKEK solution in D₂O. It turned out that snake skin is the appropriate alternative model membrane for this type of permeation test.

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A new FTIR-ATR cell for drug diffusion studies

Cited by 19 publications

References 6 publications

The Role and Potential of Vibrational Spectroscopy in the Study and Characterization of Traditional and Novel Food Packaging Structures

The Role and Potential of Vibrational Spectroscopy in the Study and Characterization of Traditional and Novel Food Packaging Structures

In situ permeation study of drug through the stratum corneum using attenuated total reflection Fourier transform infrared spectroscopic imaging

Dermal Peptide Delivery Using Enhancer Molecules and Colloidal Carrier Systems – Part IV: Search for an Alternative Model Membrane for Future ATR Permeation Studies Using PKEK as the Model Substance

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