A new four‐color flow cytometric assay to detect apoptosis in lymphocyte subsets of cultured peripheral blood cells

Hasper, H.J.; Weghorst, R.M.; Richel, Dick J.; Meerwaldt, J.H.; Olthuis, F.M.F.G.; Schenkeveld, Cees

doi:10.1002/(sici)1097-0320(20000601)40:2<167::aid-cyto11>3.3.co;2-t

Cytometry

2000

DOI: 10.1002/(sici)1097-0320(20000601)40:2<167::aid-cyto11>3.3.co;2-t

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A new four‐color flow cytometric assay to detect apoptosis in lymphocyte subsets of cultured peripheral blood cells

H.J. Hasper

R.M. Weghorst

Dick J. Richel

et al.

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2009

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“…However, this assay is relatively labor-intensive, requires 4 days incubation, and is unable to completely exclude from its results the chemosensitivity response of normal marrow lymphocytes from malignant lymphoblasts. Several recent reports suggest that techniques studying in vitro drug sensitivity using flow cytometry may be readily applicable to the study of childhood ALL [28-31]. …”

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Flow cytometric chemosensitivity assay as a predictive tool of early clinical response in acute lymphoblastic leukemia

Galderisi

Stork

et al. 2009

Pediatric Blood & Cancer

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Background Residual disease or rapidity of response to induction therapy is among the most powerful predictors of outcome in pediatric Acute Lymphoblastic Leukemia (ALL). Method Utilizing a multiparameter flow cytometric chemosensitivity assay (FCCA), we studied the relationship between in vitro drug sensitivity of diagnostic leukemic blasts from 30 children with ALL and rapidity of response to induction therapy. We also analyzed the in vitro drug sensitivity of de novo leukemic blasts among various clinical subsets. Results Compared to rapid early responders (RER), slow early responders (SER) had a significantly greater in vitro drug resistance to Dexamethasone (DEX) (P = 0.04) and Prednisone (P = 0.05). The studies with all other drugs showed a non-significant trend with the SER having a higher in vitro drug resistance compared to the RER. Risk group stratified analyses indicated that in vitro resistance to Asparaginase (ASP), DEX, and Vincristine (VCR) were each significantly related to having very high risk ALL. Additionally, a significantly higher in vitro drug resistance to ASP and VCR was associated with unfavorable lymphoblast genetics and ultimate relapse. Conclusion Our data indicate that this FCCA is a potentially simple and rapid method to detect inherent resistance to initial ALL therapy very early in induction, thus allowing for treatment modification shortly thereafter.

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confidence: 99%

Flow cytometric chemosensitivity assay as a predictive tool of early clinical response in acute lymphoblastic leukemia

Galderisi

Stork

et al. 2009

Pediatric Blood & Cancer

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A new four‐color flow cytometric assay to detect apoptosis in lymphocyte subsets of cultured peripheral blood cells

Cited by 1 publication

References 5 publications

Flow cytometric chemosensitivity assay as a predictive tool of early clinical response in acute lymphoblastic leukemia

Flow cytometric chemosensitivity assay as a predictive tool of early clinical response in acute lymphoblastic leukemia

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