A new fluorescent probe for sensing of biothiols and screening of acetylcholinesterase inhibitors

Wu, Shengjun; Yuge, Louis; Deng, Tao; Wang, Xiaojuan; Hu, Shiyou; Peng, Guiyuan; Huang, Xinan; Ling, Yanwu; Liu, Fang

doi:10.1039/d0ob00020e

Cited by 14 publications

(5 citation statements)

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“…The results showed that the fluorescence intensities versus GSH, Cys, and Hcy were linear up to 1 μM ( Figure 4 ). The limit of detections (LODs) for GSH, Cys, and Hcy were calculated as low as 13.4, 4.5, and 18.3 nM, respectively, according to the equation LOD = 3 S/K [ 29 , 30 ], where S is the standard deviation of fluorescence for blank and K is the slope of the calibration plot. These LOD and the linear range values are among the best results of those recently reported biothiol sensors ( Table S1 ), revealing high sensitivity of the present system.…”

Section: Resultsmentioning

confidence: 99%

Dual-Functional Peroxidase-Copper Phosphate Hybrid Nanoflowers for Sensitive Detection of Biological Thiols

Kim

2021

IJMS

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An effective strategy to detect biological thiols (biothiols), including glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), holds significant incentive since they play vital roles in many cellular processes and are closely related to many diseases. Here, we demonstrated that hybrid nanoflowers composed of crystalline copper phosphate and horseradish peroxidase (HRP) served as a functional unit exhibiting dual catalytic activities of biothiol oxidase and HRP, yielding a cascade reaction system for a sensitive one-pot fluorescent detection of biothiols. The nanoflowers were synthesized through the anisotropic growth of copper phosphate petals coordinated with the amine/amide moieties of HRP, by simply incubating HRP and copper(II) sulfate for three days at room temperature. Copper phosphates within the nanoflowers oxidized target biothiols to generate H2O2, which activated the entrapped HRP to oxidize the employed Amplex UltraRed substrate to produce intense fluorescence. Using this strategy, biothiols were selectively and sensitively detected by monitoring the respective fluorescence intensity. This nanoflower-based strategy was also successfully employed for reliable quantification of biothiols present in human serum, demonstrating its great potential for clinical diagnostics.

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Section: Resultsmentioning

confidence: 99%

Dual-Functional Peroxidase-Copper Phosphate Hybrid Nanoflowers for Sensitive Detection of Biological Thiols

Kim

2021

IJMS

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“…Secondly, a boronate fluorescent probe is utilized for the detection of the h202 generation [50]. Lastly, there are probes specifically designed for screening acetylcholinesterase inhibitors [51].…”

Section: Fluorescent Probes For Tissues and Organsmentioning

confidence: 99%

New Advances in the Exploration of Esterases with PET and Fluorescent Probes

Gil-Rivas,

de Pascual-Teresa,

Ortín

et al. 2023

Molecules

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Esterases are hydrolases that catalyze the hydrolysis of esters into the corresponding acids and alcohols. The development of fluorescent probes for detecting esterases is of great importance due to their wide spectrum of biological and industrial applications. These probes can provide a rapid and sensitive method for detecting the presence and activity of esterases in various samples, including biological fluids, food products, and environmental samples. Fluorescent probes can also be used for monitoring the effects of drugs and environmental toxins on esterase activity, as well as to study the functions and mechanisms of these enzymes in several biological systems. Additionally, fluorescent probes can be designed to selectively target specific types of esterases, such as those found in pathogenic bacteria or cancer cells. In this review, we summarize the recent fluorescent probes described for the visualization of cell viability and some applications for in vivo imaging. On the other hand, positron emission tomography (PET) is a nuclear-based molecular imaging modality of great value for studying the activity of enzymes in vivo. We provide some examples of PET probes for imaging acetylcholinesterases and butyrylcholinesterases in the brain, which are valuable tools for diagnosing dementia and monitoring the effects of anticholinergic drugs on the central nervous system.

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“…Among all these, the fluorescence spectroscopic assay is one of the most commonly used methods, as it has high sensitivity and easy accessibility for high-throughput screening. 9 Traditional fluorescence analysis relies on the changes of intrinsic fluorescence from HSA tryptophan residues. 10 However, the intrinsic fluorescence has a wavelength typically ranging from ultraviolet (UV) to blue light, which is easily subject to interferences due to the presence of other fluorescent biomolecules and drugs.…”

Section: Introductionmentioning

confidence: 99%

Probing the serum albumin binding site of fenamates and photochemical protein labeling with a fluorescent dye

et al. 2022

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A new fluorescent probe for sensing of biothiols and screening of acetylcholinesterase inhibitors

Abstract: An axial N2O-type BODIPY probe has been proposed for sensitive and selective sensing of biothiols and screening of AChE inhibitors using a fluorescence turn-on assay.

Cited by 14 publications

References 50 publications

Dual-Functional Peroxidase-Copper Phosphate Hybrid Nanoflowers for Sensitive Detection of Biological Thiols

Dual-Functional Peroxidase-Copper Phosphate Hybrid Nanoflowers for Sensitive Detection of Biological Thiols

New Advances in the Exploration of Esterases with PET and Fluorescent Probes

Probing the serum albumin binding site of fenamates and photochemical protein labeling with a fluorescent dye

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