A new fluorescent pH probe for acidic conditions

Zhang, Xuan; Song, Guoyong; Cao, Xiang‐Jian; Liu, Jinting; Chen, Mingyu; Cao, Xiaoqun; Zhao, Bao‐Xiang

doi:10.1039/c5ra14174e

Cited by 41 publications

(25 citation statements)

References 46 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 A variety of techniques including nuclear magnetic resonance, HPLC-MS acid-base indicator titration, absorption spectroscopy, and electrochemistry have been reported to measure pH values. [12][13][14][15] An optical probe, especially a uorescent probe, is one of the most powerful tools for detecting pH in cells for its advantages of simple operation, high sensitivity, and temporal resolution. [16][17][18] Many pH uorescent probes have been designed.…”

Section: Introductionmentioning

confidence: 99%

A “weak acid and weak base” type fluorescent probe for sensing pH: mechanism and application in living cells

et al. 2019

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

A “weak acid and weak base” type fluorescent probe for sensing pH: mechanism and application in living cells

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Thus, successful applications of small chemical molecules in inhibiting senescence in BMSCs by maintaining lysosomal functions provide potential intervention strategies to prevent body from aging and regenerate tissues [ 28 ]. Our previous work showed that a novel small molecule, 3-butyl-1-chloro imidazo [1, 5-a] pyridine-7-carboxylic acid (SGJ), had been demonstrated as a fluorescent probe to selectively and sensitively respond to acidic pH with fast response (within 3 min) [ 29 ]. Here, we wish to report our studies on SGJ, in which clarify its functions in lysosomes, relationship with Baf-A1 and role in suppressing cell senescence.…”

Section: Introductionmentioning

confidence: 99%

A pH probe inhibits senescence in mesenchymal stem cells

Wang

Han

et al. 2018

Stem Cell Res Ther

View full text Add to dashboard Cite

BackgroundBone marrow-derived mesenchymal stem cells (BMSCs) are gradually getting attention because of its multi-directional differentiation potential, hematopoietic support, and promotion of stem cell implantation. However, cultured BMSCs in vitro possess a very limited proliferation potential, and the presence of stem cell aging has substantially restricted the effect together with the efficiency in clinical treatment. Recently, increasing attention has been paid to the connection between cellular aging and lysosomal acidification as new reports indicated that vacuolar H+-ATPase (v-ATPase) activity was altered and lysosomal pH was dysregulated in the process of cellular aging. Therefore, promoting lysosomal acidification might contribute to inhibition of cell senescence. Our previous studies showed that a novel small molecule, 3-butyl-1-chloro imidazo [1, 5-a] pyridine-7-carboxylic acid (SGJ), could selectively and sensitively respond to acidic pH with fast response (within 3 min), but whether SGJ can promote lysosomal acidification and inhibit senescence in BMSCs is unknown.MethodsRat BMSCs were cultured based on our system that had been already documented. BMSCs were treated with SGJ and/or Bafilomycin-A1 (Baf-A1). The co-localization between SGJ and lysosomes was assessed by a confocal microscope. Acridine orange (AO) staining and the Lysosensor™ Green DND-189 reagents were used for indicating changes in lysosomal concentration of H+. Changes of senescence were detected by immunoblotting of p21 and senescence-associated beta-galactosidase (SA-β-gal) staining as well as immunofluorescence assay of senescence-associated heterochromatin foci (SAHF). Changes of autophagy were detected by immunoblotting of MAP1LC3 (LC3B) and SQSTM1 (p62). Cell proliferation was determined by flow cytometry. Cell viability was calculated by sulforhodamine B assay (SRB). The V0 proton channel of v-ATPase was knocked down by transfecting with its small interfering RNA (si-ATP6V0C).ResultsOur work showed that SGJ can promote lysosomal acidification and inhibit senescence in BMSCs. Firstly, SGJ and lysosomes were well co-located in senescent BMSCs with the co-localization coefficient of 0.94. Secondly, SGJ increased the concentration of H+ and the protein expression of lysosome-associated membrane protein 1 (LAMP1) and lysosome-associated membrane protein 2 (LAMP2). Thirdly, SGJ suppressed the expression of p21 in the senescent BMSCs and reduced SA-β-gal positive cells. Fourthly, SGJ promoted senescent BMSCs’ proliferation and protein level of LC3B but reduced the p62/SQSTM1 protein level. Furthermore, experimental group pretreated with 20 μM SGJ showed a stronger red fluorescent intensity, thinner cell morphology, less SA-β-gal positive cell, and less p21 protein level as well as higher cell viability in the presence of Baf-A1. Notably, ATP6V0C knockdown decreased the activity of v-ATPase and SGJ increased the concentration of H+.ConclusionOur work showed that SGJ could inhibit senescence in BMSCs and protect lysosomes by promoting ...

show abstract

“…In the case of neutral form, the dihedral angle between benzene ring and hemicyanine skeleton is only 0.30°, while the protonated form displayed a larger dihedral angle as 9.84°. Such higher energy gap and less structural rigidity of 1 + H + could be attributable to blue shift in the absorption spectra observed …”

Section: Resultsmentioning

confidence: 99%

A Colorimetric and Fluorescent pH Probe for Extremely Acidic Conditions and its Application in pH Test Paper

You¹,

Shen²,

Shen³

et al. 2018

Bulletin Korean Chem Soc

View full text Add to dashboard Cite

Here, we report a highly water‐soluble colorimetric and fluorescent pH probe that works in the pH range 0.2–3.0. The probe showed a rapid response, high stability, and excellent reversibility to acidity. Moreover, the fluorescence of probe was not influenced by the existence of high concentration of cations. The pKa of probe was calculated to be 2.25 ± 0.06. Furthermore, the pH test papers coated with the probe exhibited a distinct color change in acidic conditions.

show abstract

A new fluorescent pH probe for acidic conditions

Abstract: A new fluorescent probe based on imidazo[1,5-a]pyridine probe for low pH was synthesized and characterized.

Cited by 41 publications

References 46 publications

A “weak acid and weak base” type fluorescent probe for sensing pH: mechanism and application in living cells

A “weak acid and weak base” type fluorescent probe for sensing pH: mechanism and application in living cells

A pH probe inhibits senescence in mesenchymal stem cells

A Colorimetric and Fluorescent pH Probe for Extremely Acidic Conditions and its Application in pH Test Paper

Contact Info

Product

Resources

About