A New Fixative and Technics for the Diagnosis of Intestinal Parasites

Burrows, Robert B.

doi:10.1093/ajcp/48.3_ts.342

Cited by 33 publications

(27 citation statements)

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“…Three faecal samples were collected, one every second day, during the first week of life. These specimens were processed by Burrow's technique (Burrows, 1967).…”

Section: Methodsmentioning

confidence: 99%

Faecal excretion of rotavirus and other enteropathogens in newborns of the high and low socio-economic stratum in Santiago, Chile

et al. 1988

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SUMMARYFaecal excretion of enteropathogens was studied in newborns in their first week of life. Rotavirus was investigated in 225 neonates, of whom 107 belonged to the low socio-economic stratum (SES) and 118 to the high SES. Half of each group were delivered by caesarean section. Rotavirus was detected in 10 infants (4 4 %). Eight of them had been in the same ward and excreted the same viral electrophoretype. Enteropathogenic bacteria were isolated from 8 out of 57 (14-0 %) newborns. Positive cultures were equally distributed by SES and route of delivery. Giardia lamblia was the only parasite detected, in one infant (2-6 %) of the high SES. None of the children developed symptoms. Faecal excretion of enteropathogens ended spontaneously within a week in all cases. It is suggested that the lack of symptomatology and the spontaneous termination of the faecal excretion are related to immaturity of the small intestinal mucosa, that does not allow the completion of the steps that must take place during a successful infectious event.

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“…Three faecal samples were collected, one every second day, during the first week of life. These specimens were processed by Burrow's technique (Burrows, 1967).…”

Section: Methodsmentioning

confidence: 99%

Faecal excretion of rotavirus and other enteropathogens in newborns of the high and low socio-economic stratum in Santiago, Chile

et al. 1988

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“…Many different stains and fixatives have been used successfully with D. fragilis. Suitable fixatives include mercury-based compounds (11,75), sodium acetate-acetic acid-formalin (SAF) (74), phenol alcohol-formalin (76), modified Schaudinn's fixative (77), and thimerosal (Merthiolate)-iodine-formalin (78). A wide variety of permanent stains have been used to detect D. fragilis, including Mayer's hemalum and Lawless' stain (27), with the most common stains still in use today being iron-hematoxylin and trichrome stains (79).…”

Section: Microscopymentioning

confidence: 99%

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

et al. 2016

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SUMMARYDientamoeba fragilisis a protozoan parasite of the human bowel, commonly reported throughout the world in association with gastrointestinal symptoms. Despite its initial discovery over 100 years ago, arguably, we know less about this peculiar organism than any other pathogenic or potentially pathogenic protozoan that infects humans. The details of its life cycle and mode of transmission are not completely known, and its potential as a human pathogen is debated within the scientific community. Recently, several major advances have been made with respect to this organism's life cycle and molecular biology. While many questions remain unanswered, these and other recent advances have given rise to some intriguing new leads, which will pave the way for future research. This review encompasses a large body of knowledge generated on various aspects ofD. fragilisover the last century, together with an update on the most recent developments. This includes an update on the latest diagnostic techniques and treatments, the clinical aspects of dientamoebiasis, the development of an animal model, the description of aD. fragiliscyst stage, and the sequencing of the firstD. fragilistranscriptome.

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“…For this purpose mothers were provided with three wide-mouth vials containing PAFS (phenol-acetic-acid-formaldehyde-salt) solution. Once the collection was completed the vials were picked-up and sent to the laboratories, where they were processed according to a modification of Burrows' technique and studied for ova, cysts and trophozoites (Burrows, 1967).…”

Section: Microbiological Studiesmentioning

confidence: 99%

Acute diarrhoeal disease in children under 7 years of age in a peri-urban slum of Santiago, Chile

Araya

Figueroa

Espinoza

et al. 1985

J. Hyg.

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SUMMARYA group of 168 families who lived in a peri-urban slum in Santiago were surveyed for 9 months. All of them had a child under 7 years of age. Medical activities and data collection were carried out at a Field Station and by means of twice-weekly visits to each home, at which time cases ofdiarrhoea were recorded and investigated. Faecal samples for bacteriological, parasitological and rotavirus studies were obtained during each episode. The characteristics of clinical course, hygienic practices in the family, and monthly anthropometric measurements of infants and toddlers were also recorded. The mean monthly incidence of diarrhoea was 7-1 episodes per 100 children. Of the episodes, 44-2 Qo were associated with pathogenic bacteria, 14-4 % with rotavirus, 38-4 % with parasites and in 27 9 % no enteropathogens were identified. It was found that adequate hygienic habits were not associated with a decreased risk of developing diarrhoea and that about 600 of children did not have diarrhoea throughout the study period. The nutritional status was adequate in most cases: weight-for-age was below the 5th percentile in 115 00 of subjects and the height-for-age was normal in all. No moderate or severe cases of malnutrition were detected.

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A New Fixative and Technics for the Diagnosis of Intestinal Parasites

Cited by 33 publications

References 0 publications

Faecal excretion of rotavirus and other enteropathogens in newborns of the high and low socio-economic stratum in Santiago, Chile

Faecal excretion of rotavirus and other enteropathogens in newborns of the high and low socio-economic stratum in Santiago, Chile

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

Acute diarrhoeal disease in children under 7 years of age in a peri-urban slum of Santiago, Chile

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