A new family and genus in Dothideales for Aureobasidium-like species isolated from house dust

Humphries, Zoë; Seifert, Keith A.; Hirooka, Y.; Visagie, C.M.

doi:10.5598/imafungus.2017.08.02.05

Cited by 24 publications

(33 citation statements)

References 58 publications

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“…NR_121524 [ 28 ]) was nested within Aureobasidium ( Figure 4 A). The same result was found based on an ITS phylogeny, as well as an rpb2 phylogeny that both included ex-type sequences of K. bupleuri [ 29 ]. Both analyses also included the type species of Kabatiella , K. microsticta Bubák, which was retrieved within Aureobasidium with high support.…”

Section: Resultssupporting

confidence: 74%

Isolation and Molecular Characterization of the Romaine Lettuce Phylloplane Mycobiome

Haelewaters

Urbina

Brown

et al. 2021

JoF

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Romaine lettuce (Lactuca sativa) is an important staple of American agriculture. Unlike many vegetables, romaine lettuce is typically consumed raw. Phylloplane microbes occur naturally on plant leaves; consumption of uncooked leaves includes consumption of phylloplane microbes. Despite this fact, the microbes that naturally occur on produce such as romaine lettuce are for the most part uncharacterized. In this study, we conducted culture-based studies of the fungal romaine lettuce phylloplane community from organic and conventionally grown samples. In addition to an enumeration of all such microbes, we define and provide a discussion of the genera that form the “core” romaine lettuce mycobiome, which represent 85.5% of all obtained isolates: Alternaria, Aureobasidium, Cladosporium, Filobasidium, Naganishia, Papiliotrema, Rhodotorula, Sampaiozyma, Sporobolomyces, Symmetrospora and Vishniacozyma. We highlight the need for additional mycological expertise in that 23% of species in these core genera appear to be new to science and resolve some taxonomic issues we encountered during our work with new combinations for Aureobasidiumbupleuri and Curvibasidium nothofagi. Finally, our work lays the ground for future studies that seek to understand the effect these communities may have on preventing or facilitating establishment of exogenous microbes, such as food spoilage microbes and plant or human pathogens.

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Section: Resultssupporting

confidence: 74%

Isolation and Molecular Characterization of the Romaine Lettuce Phylloplane Mycobiome

Haelewaters

Urbina

Brown

et al. 2021

JoF

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“…In our case, the edited sequence was compared via BLAST to GenBank nucleotide collections, and the obtained highest-scoring pair (100% of identity) allowed the identification of the isolated microorganism as Zalaria obscura. In addition, our sequence showed a 100% identity to the sequences reported by Humphries et al [22] that have introduced a new family (Zalariaceae) and a new genus (Zalaria) with two species, Z. alba and Z. obscura, to accommodate this relatively novel species.…”

Section: Resultssupporting

confidence: 73%

Isolation and molecular identification of a strain belonging to the new species Zalaria obscura from a deteriorated wooden artwork

et al. 2020

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We report the case of an outdoor deteriorated wooden sculpture of Madonna, completely blackened in the face, and thus suspected of fungal attack. A multidisciplinary approach, including microbiological analysis, molecular biology, and Fourier transform infrared (FT-IR) spectroscopy, was applied to understand the real nature of the observed alteration. FT-IR showed that the blackening was due to the application of a natural terpene resin subjected to alteration over time. The microbiological assay allowed to isolate a particular black fungus that has been recovered in the vegetative phase, growing as the only species adapted to the examined substrate. Basic Local Alignment Search Tool (BLAST) analysis of the ITS (internal transcribed spacer) region sequence identified the fungus (LS31012019) as Zalaria obscura, a black yeast belonging to the new genus Zalaria, family Dothideales. Overall, this study evidenced the importance of a multidisciplinary approach to understand the real causes of observed deterioration of artworks. More interestingly, the recovery of a strain identified as Z. obscura from this type of substrate is never reported in the literature and this finding could offer the possibility to investigate the role of this microorganism in the deterioration process of cultural heritage.

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“…Strains of A. melanogenum can be isolated from diverse, mainly oligotrophic and aqueous habitats [5]-they were found in hypersaline water [7], in Greenland glacial ice [8], but also in association with plants [9,10] and in desert soil [11]. Importantly, A. melanogenum can colonize indoor environments and is found often in large numbers in washing machines [12], dishwashers [13], tap water [14], house dust [15], and food [16].…”

Section: Introductionmentioning

confidence: 99%

Virulence Traits and Population Genomics of the Black Yeast Aureobasidium melanogenum

Černoša

Sun²,

Fang³

et al. 2021

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The black yeast-like fungus Aureobasidium melanogenum is an opportunistic human pathogen frequently found indoors. Its traits, potentially linked to pathogenesis, have never been systematically studied. Here, we examine 49 A. melanogenum strains for growth at 37 °C, siderophore production, hemolytic activity, and assimilation of hydrocarbons and human neurotransmitters and report within-species variability. All but one strain grew at 37 °C. All strains produced siderophores and showed some hemolytic activity. The largest differences between strains were observed in the assimilation of hydrocarbons and human neurotransmitters. We show for the first time that fungi from the order Dothideales can assimilate aromatic hydrocarbons. To explain the background, we sequenced the genomes of all 49 strains and identified genes putatively involved in siderophore production and hemolysis. Genomic analysis revealed a fairly structured population of A. melanogenum, raising the possibility that some phylogenetic lineages have higher virulence potential than others. Population genomics indicated that the species is strictly clonal, although more than half of the genomes were diploid. The existence of relatively heterozygous diploids in an otherwise clonal species is described for only the second time in fungi. The genomic and phenotypic data from this study should help to resolve the non-trivial taxonomy of the genus Aureobasidium and reduce the medical hazards of exploiting the biotechnological potential of other, non-pathogenic species of this genus.

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A new family and genus in Dothideales for Aureobasidium-like species isolated from house dust

Cited by 24 publications

References 58 publications

Isolation and Molecular Characterization of the Romaine Lettuce Phylloplane Mycobiome

Isolation and Molecular Characterization of the Romaine Lettuce Phylloplane Mycobiome

Isolation and molecular identification of a strain belonging to the new species Zalaria obscura from a deteriorated wooden artwork

Virulence Traits and Population Genomics of the Black Yeast Aureobasidium melanogenum

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