A new experimental model of glaucoma in rats through intracameral injections of hyaluronic acid

Moreno, Maria Cecilia Rey; Marcos, Hernán J. Aldana; Croxatto, J. Oscar; Sande, Pablo H.; Campanelli, Julieta; Jaliffa, Carolina O.; Benozzi, Jorge; Rosenstein, Ruth E.

doi:10.1016/j.exer.2005.01.008

Cited by 91 publications

(70 citation statements)

References 19 publications

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“…First, the current experimental model is an acute OHT model that mimics only some features of glaucoma, such as variable IOP profile and pressure-induced RGC loss [47]. However, chronicity, as seen in primary open-angle glaucoma, is not present in our acute experimental model.…”

Section: Discussionmentioning

confidence: 98%

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Neuroprotective effects of intravitreally transplanted adipose tissue and bone marrow–derived mesenchymal stem cells in an experimental ocular hypertension model

et al. 2015

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Section: Discussionmentioning

confidence: 98%

“…The injections were made through the corneal limbus into the anterior chamber to create a self-sealing entry. The needle was moved in a clockwise direction, and contact of the needle tip with the iris and the lens was avoided [47]. Induction of OHT was repeated at 1-week intervals for 3 weeks [50,51].…”

Section: Induction Of Ocular Hypertensionmentioning

confidence: 99%

Neuroprotective effects of intravitreally transplanted adipose tissue and bone marrow–derived mesenchymal stem cells in an experimental ocular hypertension model

et al. 2015

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“…Electroretinographic activity was assessed in dark-adapted rodents as previously described (Moreno et al, 2005). Briefly, after 6 h of dark adaptation, mice were anesthetized with an intraperitoneal injection of ketamine hydrochloride and xylazine hydrochloride under dim red illumination.…”

Section: Electroretinographymentioning

confidence: 99%

Circadian modulation of interval timing in mice

et al. 2011

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Temporal perception is fundamental to environmental adaptation in humans and other animals. To deal with timing and time perception, organisms have developed multiple systems that are active over a broad range of order of magnitude, the most important being circadian timing, interval timing and millisecond timing. The circadian pacemaker is located in the suprachiasmatic nuclei (SCN) of the hypothalamus, and is driven by a selfsustaining oscillator with a period close to 24 h. Time estimation in the second-to-minutes range -known as interval timing -involves the interaction of the basal ganglia and the prefrontal cortex. In this work we tested the hypothesis that interval timing in mice is sensitive to circadian modulations. Animals were trained following the peak-interval (PI) procedure. Results show significant differences in the estimation of 24-second intervals at different times of day, with a higher accuracy in the group trained at night, which were maintained under constant dark (DD) conditions. Interval timing was also studied in animals under constant light (LL) conditions, which abolish circadian rhythmicity. Mice under LL conditions were unable to acquire temporal control in the peak interval procedure.Moreover, short time estimation in animals subjected to circadian desynchronizations (modeling jet lag-like situations) was also affected. Taken together, our results indicate that short-time estimation is modulated by the circadian clock.© 2010 Elsevier B.V. All rights reserved. Keywords:Circadian rhythms Interval timingBasal ganglia Suprachiasmatic nuclei IntroductionTiming and time perception are fundamental to survival and goal reaching in humans and other animals. Organisms have developed diverse mechanisms for timing across different scales, the most important being circadian timing, interval timing and millisecond timing . The circadian pacemaker -which is driven by a self-sustaining oscillator with a period close to 24 h -is located in the suprachiasmatic nuclei (SCN) of the hypothalamus (Dunlap et al., 2004), and the principal signal that adjusts its activity is the light-dark cycle (Morin and Allen, 2006;Golombek and Rosenstein, 2010). The molecular mechanism of the endogenous circadian clock is comprised by interlocked transcription-translation feedback loops (Reppert and Weaver, 2002). On the other hand, the perception of shorter durations in the seconds-to-minutes range, known as interval timing, is crucial to learning, memory, decision making and other cognitive

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“…Unilateral glaucoma was induced in the right eye by the injection of 30 lL hyaluronic acid (HA) (10 mg/mL) into the anterior chamber through the clear cornea, near the corneoscleral limbus, using a hypodermic needle (22 gauge) once per week for 4 or 5 weeks on the same calendar day and at the same time, according to the protocol of Moreno et al 21 The rats were anesthetized intramuscularly with ketamine (70 mg/ kg) and xylazine (10 mg/kg). In addition, two drops of 0.4% benoxinate hydrochloride was instilled directly on the cornea as a topical anesthetic.…”

Section: Induction Of Glaucomamentioning

confidence: 99%

Antiglaucomatous Effects of the Activation of Intrinsic Angiotensin-Converting Enzyme 2

Foureaux

Nogueira

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To evaluate the effects of the activation of endogenous angiotensin-converting enzyme 2 (ACE2) using the compound diminazene aceturate (DIZE) in an experimental model of glaucoma in Wistar rats.METHODS. DIZE (1 mg/kg) was administered daily, either systemically or topically, and the IOP was measured weekly. To examine the role of the Mas receptor in the effects of DIZE, the Ang-(1-7) antagonist A-779 was co-administered. Drainage of the aqueous humor was evaluated by using scintigraphy. The analysis of ACE2 expression by immunohistochemistry and the counting of retinal ganglion cells (RGCs) were performed in histologic sections. Additionally, the nerve fiber structure was evaluated by transmission electron microscopy. RESULTS.The systemic administration and topical administration (in the form of eye drops) of DIZE increased the ACE2 expression in the eyes and significantly decreased the IOP of glaucomatous rats without changing the blood pressure. Importantly, this IOP-lowering action of DIZE was similar to the effects of dorzolamide. The antiglaucomatous effects of DIZE were blocked by A-779. Histologic analysis revealed that the reduction in the number of RGCs and the increase in the expression of caspase-3 in the RGC layer in glaucomatous animals were prevented by DIZE. This compound also prevented alterations in the cytoplasm of axons in glaucomatous rats. In addition to these neuroprotective effects, DIZE facilitated the drainage of the aqueous humor. CONCLUSIONS.Our results evidence the pathophysiologic relevance of the ocular ACE2/Ang-(1-7)/Mas axis of the renin-angiotensin system and, importantly, indicate that the activation of intrinsic ACE2 is a potential therapeutic strategy to treat glaucoma.

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A new experimental model of glaucoma in rats through intracameral injections of hyaluronic acid

Cited by 91 publications

References 19 publications

Neuroprotective effects of intravitreally transplanted adipose tissue and bone marrow–derived mesenchymal stem cells in an experimental ocular hypertension model

Neuroprotective effects of intravitreally transplanted adipose tissue and bone marrow–derived mesenchymal stem cells in an experimental ocular hypertension model

Circadian modulation of interval timing in mice

Antiglaucomatous Effects of the Activation of Intrinsic Angiotensin-Converting Enzyme 2

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