A new experimental model for studying the response of periodontal ligament cells to hydrostatic pressure

Yousefian, Joseph; Firouzian, Farzad; Shanfeld, Joseph; Ngan, Peter; Lanese, Richard R.; Davidovitch, Zéev

doi:10.1016/s0889-5406(95)70038-2

Cited by 57 publications

(24 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…either increase (Yousefian et al, 1995;Matsuda et al, 1998b) or inhibit proliferation (Hao et al, 2009). In our microarray analysis, DPT, ASCL2, APOE, BTG1, NPPA, and SPP1, which were involved in negative regulation of cell proliferation, were up-regulated.…”

mentioning

confidence: 79%

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

Han

Sheng

et al. 2013

DNA and Cell Biology

View full text Add to dashboard Cite

Physiological occlusal force constitutively exists in the oral environment and is important for periodontal homeostasis and remodeling. Cyclic tensile stress (CTS) triggers the biological response of periodontal ligament (PDL). However, a few reports have studied the correlation between CTS during physiological occlusal force and PDL cell activities such as osteogenic differentiation. In the present study, human PDL cells (hPDLCs) were subjected to 10% elongation CTS loading at 0.5 Hz for 24 h, which represents the physiological conditions of occlusal force. Gene expression microarray was used to investigate the mechano-induced differential gene profile and pathway analysis in vitro. The osteogenic relative factors, that is, SPP1, RUNX2, and SP7, were assessed by real-time PCR and Western blot. The involvement of mitogen-activated protein kinase (MAPK) signaling pathways was investigated by Western blot with a specific inhibitor. The expressions of SPP1, RUNX2, SP7, p-ERK1/2, and p-Elk1 were up-regulated after 10% CTS exposure. However, these up-regulated expressions were prevented by ERK1/2 inhibitor U0126 in the physiological occlusal force-applied hPDLCs. These results showed that 10% CTS could enhance osteogenic differentiation of hPDLCs via ERK1/2-Elk1 MAPK pathway, indicating that CTS during physiological occlusal force is a potent agent for PDL remodeling.

show abstract

mentioning

confidence: 79%

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

Han

Sheng

et al. 2013

DNA and Cell Biology

View full text Add to dashboard Cite

show abstract

“…The blot was reacted with rabbit anti-human elastin antibody. 132 pressure and compressive forces, cells respond to optimal levels of mechanical stimulation (Yousefian et al, 1995;Vouyouka et al, 1998;Haberstroh et al, 1999;Schwartz et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Hydrostatic pressure stimulates synthesis of elastin in cultured optic nerve head astrocytes

Hernandez

Peña

Selvidge

et al. 2000

Glia

View full text Add to dashboard Cite

“…To simulate a compressive stress to the cultured chondrocytes, we applied a self-designed, hydraulic pressure-controlling cellular-strain unit that followed the model developed by Yousefian et al [1995]. The pressure was generated by continuously compressing the gas phase (2% CO 2 in air) in a closed culture chamber (98% humidity) that was placed in a 37 ° C incubator.…”

Section: Mechanical Stimulation Of Bmscsmentioning

confidence: 99%

Estrogen and Its Receptor Enhance Mechanobiological Effects in Compressed Bone Mesenchymal Stem Cells

Zhang

Chen²,

Wang³

et al. 2011

Cells Tissues Organs

View full text Add to dashboard Cite

Mechanical stimulation and estrogen have been proven to be two important factors in promoting mesenchymal stem cell activity, which is closely associated with bone formation, mass maintenance and remodeling. However, the superposition effects of mechanical stimulation and estrogen on stem cells remain unknown. It is also unclear if the estrogen receptor (ER) plays only a key role in estrogen signaling or if it is also involved in the mechanotransduction of stem cells. To investigate the role of estrogen and its receptors in the mechanobiological effects in bone mesenchymal stem cells (BMSCs), isolated mesenchymal stem cells from bone marrow were exposed to mechanical pressure under additional estrogen treatment or ER blockade. Cell proliferation was examined using an MTT assay and alkaline phosphatase (ALP) activity was determined by a modified enzyme kinetic method. Alignment of the cytoskeleton was observed by Coomassie brilliant blue staining and F-actin fluorescent staining. Cellular ultrastructure was observed under transmission electron microscope. Expression of ERα was investigated using Western blot analysis. Results indicated that mechanical pressure promoted cell proliferation, ALP activity, ERα expression and F-actin stress fiber formation. Overall, this effect was enhanced by the addition of estrogen and inhibited by ER blockade. We concluded that pressure stimulated proliferation and differentiation capability via F-actin transduction in BMSCs. The effects were enhanced by the addition of estrogen, and the ER plays an important role in regulating mechanobiological effects and the mechanotransduction processes of BMSCs.

show abstract

A new experimental model for studying the response of periodontal ligament cells to hydrostatic pressure

Cited by 57 publications

References 20 publications

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

Hydrostatic pressure stimulates synthesis of elastin in cultured optic nerve head astrocytes

Estrogen and Its Receptor Enhance Mechanobiological Effects in Compressed Bone Mesenchymal Stem Cells

Contact Info

Product

Resources

About