A New Escherichia Coli O-Group, O159, Associated with Outbreaks of Enteritis in Infants

Rj, Gross; Rowe, B.; Henderson, A.; Me, Byatt; Jc, Maclaurin

doi:10.3109/inf.1976.8.issue-3.14

Cited by 33 publications

(10 citation statements)

References 10 publications

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“…'Toxigenic control and enteropathogenic strains were perfused at a concentration of 10 ng/ml; nontoxigenic control strains were perfused at 100 fig/ml. be responsible for outbreaks of epidemics of diarrhea in nurseries and in confined adult groups (1,19,25,41). Second, enteropathogenic serotypes which have been clearly incriminated by epidemiological evidence as the causative factor responsible for nursery epidemics, but which were negative on routine testing for enterotoxigenicity, have been shown in the present study to produce toxins or related toxic substances that induce water secretion.…”

Section: Discussionmentioning

confidence: 47%

Enterotoxigenicity of Enteropathogenic Serotypes of Escherichia coli Isolated from Infants with Epidemic Diarrhea

Klipstein

Rowe²,

Engert

et al. 1978

Infect Immun

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Enteropathogenic serotypes of Escherichia coli which have been incriminated by epidemiological evidence as responsible for epidemics of acute diarrhea in infants are often found to be nontoxigenic when tested by conventional systems such as Y1-adrenal, Chinese hamster ovary, and suckling mouse assays. Twelve such strains, representing four different enteropathogenic serotypes, were examined for their capacity to elaborate toxic materials which alter water transport. Ultrafiltration fractions prepared to contain either a high-molecular-weight, heatlabile or a low-molecular-weight, heat-stable form of toxin from each strain were perfused through rat jejuna in graded concentrations ranging from 100 ftg to 0.1 ng/ml. Ten of the twelve enteropathogenic strains produced one or both toxin forms that induced water secretion at concentrations of 1 to 10 ng/ml. Values in this range are considered indicative of clinically significant enterotoxigenicity in this assay system, and toxins from well-documented toxigenic strains examined in this study were active at these same concentrations. Similar preparations from ten control strains from healthy persons were either inactive or evoked water secretion only at concentrations of 10 to 100 ,g/ml. These observations suggest that enteropathogenic serotypes of E. coli isolated from epidemics of infantile diarrhea produce diarrhea by elaborating potent heat-labile and heat-stable toxin forms which alter water transport but which are inactive in conventional assay systems. The manner in which these toxins differ either quantitatively or qualitatively from those which stimulate the conventional test systems is unknown.on August 5, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Discussionmentioning

confidence: 47%

Enterotoxigenicity of Enteropathogenic Serotypes of Escherichia coli Isolated from Infants with Epidemic Diarrhea

Klipstein

Rowe²,

Engert

et al. 1978

Infect Immun

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“…The other two antisera used indicated the presence of ETEC in eight samples but none of these yielded ETEC by DNA probing. These antisera failed to detect the presence of ETEC belonging to 0 serogroups 25,128 and 148. ETEC strains belonging to the other seven serogroups identified were obtained from samples in which intestinal pathogens were not reported initially.…”

Section: Properties Of Etec Strains Isolatedmentioning

confidence: 95%

“…Strains of E. coli 0159 have been found in studies of travellers' diarrhoea [6], in surveys of the occurrence of human ETEC in underdeveloped areas [24] and from outbreaks of E. coli-associated diarrhoea [25,26]. These strains had the flagellar antigens 20 or 37, or were non-motile.…”

Section: Properties Of Etec Strains Isolatedmentioning

confidence: 99%

Isolation of enterotoxigenicEscherichia colifrom British troops in Saudi Arabia

Willshaw¹,

Cheasty²,

Rowe³

et al. 1995

Epidemiol. Infect.

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SUAIMARYSpecimens from 181 patients with diarrhoea were examined by a Military General Hospital in a 3-month period during deployment of troops to Saudi Arabia in 1990/1. DNA probes for heat labile (LT) and heat stable (ST) enterotoxin genes identified enterotoxigenic Escherichia coli (ETEC) in 47 of the specimens (26%) and 49 ETEC strains were isolated. The majority (55%) belonged to a novel ETEC serotype having the 0-antigen 159 and a flagellar antigen designated as a provisional new type. They produced ST and the coli surface associated antigen (CS)6. Strains of serotype 06: H16 represented 22 % of the ETEC examined. They produced ST, LT and C53 together with either CS1 or C52. The remaining ETEC belonged to seven 0: H serotypes. Overall, ST was the only enterotoxin gene identified in 73 % of the ETEC and 67 % of the strains expressed CS6 in the absence of other colonization antigens. Resistance to three or more antibiotics was observed in 53 % of the ETEC, including most of the 0159 strains.

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“…Bacterial agents have mostly been reported to cause outbreaks of nosocomial gastroenteritis. These include Salmonella species (Hammami et al, 1991), C. difficile (Pépin et al, 2004), Shigella species (Paton et al, 1991), Yersinia species (Jarvis, 1992), enteropathogenic Escherichia coli (EPEC) (Boyer et al, 1975;Gross et al, 1976), enterotoxigenic E. coli (ETEC) (Gross et al, 1976) and Vibrio cholerae (Ryder et al, 1986). The most common sources of the organism are an infected infant, the hands of hospital personnel or contaminated articles.…”

Section: Introductionmentioning

confidence: 99%

Diarrhoeagenic Escherichia coli as a predominant cause of paediatric nosocomial diarrhoea in India

Chandra

Singh

Taneja

et al. 2012

Journal of Medical Microbiology

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Intestinal nosocomial infections remain a major concern in paediatric wards leading to increased morbidity and mortality. This study determined the aetiological and epidemiological profile of nosocomial diarrhoea (ND) among children admitted to a hospital in India. During the period of January 2008 to June 2009, we consecutively enrolled 100 children between the age of 2 months and 14 years who developed ND as defined by the Centers for Disease Control and Prevention. A control group of patients matched for age and severity score but with no diarrhoea at admission or during their hospital stay (n550) were also enrolled. Stool samples were cultured for various pathogens using standard protocols. Clostridium difficile toxins and rotavirus antigen were detected using commercial ELISAs. Detection of diarrhoeagenic Escherichia coli was carried out by multiplex PCR assay. All patient details were noted. In this study, males predominated (77 %), and 56 % children were ,1 year of age and 96 % were ,5 years. The mean duration of diarrhoea and hospitalization in the case group was 3.2 days and 27.5 days, respectively. Malignancy and nasogastric tube usage were significant underlying factors for the development of ND. Diarrhoeagenic E. coli was the commonest agent (47 %: enterotoxigenic E. coli, enteroaggregative E. coli and enteropathogenic E. coli were isolated in 22, 18 and 7 % of patients, respectively). C. difficile toxin was seen in 9 % of cases, whilst rotavirus was found in 8 % of cases. Although rotavirus and C. difficile are major causative agents of hospital-acquired diarrhoea in the developed world, in this setting diarrhoeagenic E. coli was responsible for the majority of cases of hospital-acquired diarrhoea. ND was most common in children aged ,5 years.

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A New Escherichia Coli O-Group, O159, Associated with Outbreaks of Enteritis in Infants

Cited by 33 publications

References 10 publications

Enterotoxigenicity of Enteropathogenic Serotypes of Escherichia coli Isolated from Infants with Epidemic Diarrhea

Enterotoxigenicity of Enteropathogenic Serotypes of Escherichia coli Isolated from Infants with Epidemic Diarrhea

Isolation of enterotoxigenicEscherichia colifrom British troops in Saudi Arabia

Diarrhoeagenic Escherichia coli as a predominant cause of paediatric nosocomial diarrhoea in India

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