A New Eremophilane Sesquiterpene from Cultures of the Fungus Pholiota nameko

Shao, Lin-Jiao; Lou, Dengji; Li, Yin-Fei; Liang, Ying-Zhong; Niu, Wan-Rong; Guo, Min; Yang, Xiaoyan

doi:10.1007/s10600-022-03710-0

Cited by 2 publications

(2 citation statements)

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“…On the other hand, the Pholiota nameko coated with Pseudomonas BAS experienced a faster rate of autolysis throughout the 3 days, while the autolysis rate of the control group decreased over the same period. The most rapid autolysis rate was observed in the Pholiota nameko at ( Kawamura et al, 2007 ; Abreu et al, 2021 ; Shao et al, 2022 ; Zhang et al, 2022 ) days, with a sharp increase from 0.8 to 3.5. By day 6, the majority of the Pholiota nameko had undergone autolysis and decomposed, as evidenced by the presence of black sap, reflected by an autolysis index of 3.7.…”

Section: Resultsmentioning

confidence: 97%

“…It is abundant in crude protein, fat, carbohydrates, crude fibers, ash, calcium, phosphorus, iron, vitamin B (VB), vitamin (VC), niacin, and other essential amino acids, all of which contribute positively to human health ( Kawamura et al, 2007 ). Furthermore, Pholiota nameko ranks among the top 10 globally traded mushrooms and has been recommended by the Food and Agriculture Organization for cultivation in underdeveloped nations ( Shao et al, 2022 ). With a history spanning over 6,000 years, Pholiota nameko has gained immense popularity as both a food and a medicinal mushroom.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of microbial community evolution, autolysis phenomena, and energy metabolism pathways in Pholiota nameko endophytes

Zhao,

He,

Wang

et al. 2024

Front. Microbiol.

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IntroductionPholiota nameko is a widely consumed edible fungus. This study focuses on two crucial developmental stages of Pholiota nameko, namely, mycelium and ascospores. The objectives of this research were to investigate changes in microbial diversity and community structure during the growth of Pholiota nameko and to analyze the adaptability of the dominant strains to their respective habitats through metabolic.MethodsSpecifically, we conducted second-generation sequencing of the 16S rRNA gene (Illumina) on samples obtained from these stages. In addition, we isolated and characterized endophytes present in Pholiota nameko, focusing on examining the impact of dominant endophyte genera on autolysis. We also conducted a metabolic pathway analysis.Results and discussionThe results unveiled 578,414 valid sequences of Pholiota nameko endophytic fungi. At the phylum level, the dominant taxa were Basidiomycota, Ascomycota, Zoopagomycota, and Mucoromycota. At the genus level, the dominant taxa observed were Pholiota, Inocybe, Fusarium, and Hortiboletus. For endophytic bacteria, we obtained 458,475 valid sequences. The dominant phyla were Proteobacteria, TM6, Firmicutes, and Bacteroidetes, while the dominant genera were Edaphobacter, Xanthomonas, Burkholderia, and Pseudomonas. Moreover, we identified the isolated strains in Pholiota nameko using 16S rDNA, and most of them were found to belong to the genus Pseudomonas, with Pseudomonas putida being the most prevalent strain. The findings revealed that the Pseudomonas putida strain has the ability to slow down the breakdown of soluble proteins and partially suppress the metabolic processes that generate superoxide anion radicals in Pholiota nameko, thereby reducing autolysis. Additionally, our results demonstrated that molybdenum enzyme-mediated anaerobic oxidative phosphorylation reactions were the primary energy metabolism pathway in the Pseudomonas putida strain. This suggests that the molybdenum cofactor synthesis pathway might be the main mechanism through which Pholiota nameko adapts to its complex and diverse habitats.

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