A New Enzyme Preparation for Reducing the Viscosity of Whole-Grain Rye Extracts

Рожкова, А. М.; Miarzlou, Dzmitry A; Bashirova, Anna V.; Зоров, И. Н.; Короткова, О. Г.; Шашков, И. А.; Sinitsyn, A. P.

doi:10.3103/s0027131418020141

Cited by 3 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1). The homologies between the KIT-19 16S rDNA sequence and the 16S rDNA sequences of strains Microbulbifer elongatus [10] and Microbulbifer salipaludis [11] were 99. including alginate lyase, to cause a decrease in substrate viscosity [12,13,14,15] . This experimental result suggested the possibility that ALG19 is an endo-type enzyme.…”

Section: Description Of Microbulbifer Sp Kit-19mentioning

confidence: 99%

Purification and characterization of an alginate lyase from marine bacterium Microbulbifer sp. KIT−19

Hakamada

Syouyama

2020

J. Biol. Macromol.

View full text Add to dashboard Cite

Alginate-degrading bacteria were isolated from seawater and a-systematic classification was performed using 16S rDNA sequence. The isolated strain was identified as Microbulbifer sp. strain KIT-19. The properties of alginate lyase (ALG19) derived from the strain KIT-19 were investigated. ALG19 was purified by various column chromatography techniques, with specific activity of 10.1 units/mg and 7.08-fold purity. When the substrate was sodium alginate, the optimum pH of the purified ALG19 was 8.5 and optimum temperature was 40°C. The activity of the purified ALG19 increased by 2.34-fold in the presence of 100 mM Na +. Alginate is composed of β-D-mannuronic acid (M) and 1,4-linked α-L-guluronic acid (G) residues. The activity toward poly-M blocks and poly-G blocks of purified ALG19 on the basis of relative activity scale were 123% and 91% respectively, when the decomposed activity to sodium alginate was 100%. Since ALG19 was poly-M blocks specific alginate lyase, it was thought that ALG19 belongs to the polysaccharide lyase (PL)-7 family. ALG19 could be considered to be an endo-type enzyme because it lowers the kinematic viscosity of sodium alginate from 23.5 to 1.65 cSt (mm 2 /s).

show abstract

Section: Description Of Microbulbifer Sp Kit-19mentioning

confidence: 99%

Purification and characterization of an alginate lyase from marine bacterium Microbulbifer sp. KIT−19

Hakamada

Syouyama

2020

J. Biol. Macromol.

View full text Add to dashboard Cite

show abstract

Production of Industrial Enzymes based on the Expression System of the Penicillium verruculosum Fungus

Синицына

Рожкова

2020

Biiotehnologiâ (Mosk.)

View full text Add to dashboard Cite

The review provides the data on the creation of highly active recombinant strains producers of a wide range of enzymes for various fields of industry and agriculture using the expression system of the Penicillium verruculosum filamentous fungus: endoglucanases, β-glucanases, xylanases, mannanases, phytases, α-galactosidases, pectin lyases, polygalacturonases, dextranases, exo-inulinases and chitinases. The recipient strain, P. verruculosum B1-537(ΔniaD), has a high secretory capacity (up to 60 g/L of extracellular protein in the culture liquid), and the recombinant producer strains are characterized by high productivity of target enzymes. The obtained enzyme preparations contain, as a rule, 30--70% of the target recombinant enzymes (in some cases, up to 80%) of the total protein pool. The P. verruculosum expression system makes it easy to transform expression constructs containing target heterologous or homologous genes, functionally linked to the promoter and terminator of the strong inducible promoter of the cellobiohydrolase-1 (cbh1) gene, and the genetic transformation of the recipient strain can be carried out simultaneously using several vector constructs. The incorporation of target genes into the chromosome of the recipient strain is an integrative process, and the foreign recombinant proteins encoded by them are not subjected to proteolysis. The technique of primary selection of positive transformants on agar media, as well as secondary selection during their cultivation in shaking flasks and fermenters, is simple and reliable in use, partly owing to standardized parameters: the nutrient composition of the fermentation medium and the culturing conditions of recombinant strains producing various target enzymes. The time spent on creating a producer of the target enzyme, from setting the problem to obtaining a recombinant strain, takes from 3 to 6 months. It is important to emphasize that recombinant P. verruculosum strains are successfully used in industrial processes as producers of various target enzymes. expression system, cloning, industrial enzymes, feed additives, Penicillium verruculosum The work was supported by the Ministry of Science and Higher Education of the Russian Federation (State Assignment 0104-2019-0009).

show abstract

Carbohydrases: 50 Years of Research at the Department of Chemical Enzymology of Moscow State University—History and Prospects

Sinitsyn,

Sinitsyna,

Zorov

et al. 2023

Moscow Univ. Chem. Bull.

View full text Add to dashboard Cite

A New Enzyme Preparation for Reducing the Viscosity of Whole-Grain Rye Extracts

Cited by 3 publications

References 10 publications

Purification and characterization of an alginate lyase from marine bacterium Microbulbifer sp. KIT−19

Purification and characterization of an alginate lyase from marine bacterium Microbulbifer sp. KIT−19

Production of Industrial Enzymes based on the Expression System of the Penicillium verruculosum Fungus

Carbohydrases: 50 Years of Research at the Department of Chemical Enzymology of Moscow State University—History and Prospects

Contact Info

Product

Resources

About