A New Enzyme Immunoassay Specific for Blastomycosis

Lo, Ching Y.; Notenboom, Robert

doi:10.1164/ajrccm/141.1.84

Cited by 15 publications

(6 citation statements)

References 5 publications

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“…Immunodiffusion (ID) assays are frequently used for detection of tube precipitin (TP) and complement fixation (CF) antibody to C. immitis antigens in patients (14)(15)(16). However, limitations in the successful clinical application of these and other methods of detection of serum antibodies in coccidioidomycosis are recognized (18,25). The shortcomings are in part due to a lack of precise compositional data on the C. immitis antigens employed which makes standardization of the tests difficult to establish, as well as to serologic cross-reactivity with sera derived from patients with histoplasmosis, blastomycosis, and certain nonmycotic conditions (21).…”

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confidence: 99%

Antigen complex of Coccidioides immitis which elicits a precipitin antibody response in patients

1991

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The occurrence in patients of elevated levels of immunoglobulin M (IgM) precipitin antibody to Coccidioides immitis antigens, which are commonly detected by the immunodiffusion-tube precipitin (TP) assay, is suggestive of primary nondisseminating coccidioidomycosis. We previously demonstrated that the concanavalin Abound mycelial culture filtrate plus lysate preparation is a source of at least two TP antibody-reactive antigens (TP-Ags), which were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis as 120and 110-kDa fractions. Evidence is presented here that the crude filtrate plus lysate preparation contains additional lectin-bound, TP antibody-reactive fractions as well as a component which elicits a complement fixation antibody response in patients. The 120and 110-kDa fractions were isolated from the antigen complex and further characterized in this paper. Both TP-Ags are glycoproteins and have been shown by immunoelectron microscopy to be colocalized within cytoplasmic vesicles and the wall of spherules. Deglycosylation of these TP-Ags by sodium periodate treatment resulted in a loss in patients of 82 to 95% of IgM adsorption to the antigens as detected by the enzyme-linked immunosorbent assay (ELISA). Comparison of their carbohydrate compositions revealed that mannose and glucose are the predominant monosaccharides of both TP-Ags but only the 120-kDa fraction contained 3-O-methylmannose, a sugar which appears to be unique to C. immitis among the systemic fungal pathogens. We previously showed that 3-O-methylmannose is at least partly responsible for the reactivity of IgM antibody with the 120-kDa TP-Ag. Good correlation was shown between results of immunodiffusion-TP assays and ELISAs of IgM response to both the 120and 110-kDa fractions by using 70 serum samples from patients with proved coccidioidomycosis. However, only 2.8% (3 of 109) of the serum samples from patients with other mycoses and nonmycotic infections showed IgM adsorption to the 120-kDa TP-Ag as detected by the ELISA, while 21.1% (23 of 109) showed IgM adsorption to the 110-kDa TP-Ag. The 120-kDa TP-Ag is a potentially valuable serodiagnostic reagent for detection of specific IgM by ELISA in patients with primary coccidioidomycosis.

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confidence: 99%

Antigen complex of Coccidioides immitis which elicits a precipitin antibody response in patients

1991

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“…In our patient, besides steroid immunosuppression, there appears to be no other predisposing factor, although the rapidity of the clinical course precluded a detailed workup of the immunological status. Blastomycosis has been described in the setting of the AIDS [ 11,17]; in our patient, there is no evidence to suggest this as a possible predisposing factor; furthermore, besides this uncommon fungal pathogen, no other opportunistic infections are identified.…”

Section: Discussionmentioning

confidence: 52%

Fulminant blastomycosis with blastomycotic infection of a cerebral glioma

1991

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Except for isolated case reports, blastomycosis has not been identified as a significant problem in immunosuppressed patients. We describe an unusual case with blastomycotic infection of a cerebral glioma in a 56-year-old man who underwent radiotherapy for his tumor and died of fulminant blastomycotic pneumonia. This is believed to be the first reported case of Blastomyces dermatitidis infection of a cerebral glioma. The light microscopic and ultrastructural features of B. dermatitidis, the giant forms of which were encountered in our patient, are described, and thr role of immunosuppression due to steroid therapy in the pathogenesis of this fulminant infection are reviewed.

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“…A new sandwich immunoassay technique was reported to have high sensitivity and specificity, but is not commercially available. 21 The larynx may be involved via a hematogenous route from quiescent pulmonary disease, or alternatively, fungus-laden sputum traversing the larynx may cause infection by direct inoculation. 22 Dennis, 7 in 1918, reported the first case of laryngeal involvement in the English-language literature.…”

Section: Discussionmentioning

confidence: 99%

Laryngeal Blastomycosis: A Commonly Missed Diagnosis Report of Two Cases and Review of the Literature

Hanson

Spector

El‐Mofty

2000

Ann Otol Rhinol Laryngol

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Blastomycosis is a relatively uncommon fungal disease that most commonly affects the lungs. Other organs may be involved, usually secondary to dissemination of the organism. Laryngeal blastomycosis may occur in isolation from active pulmonary disease. The signs, symptoms, clinical features, and pathological findings of laryngeal blastomycosis mimic those of squamous cell carcinoma. Misdiagnosis may result in inappropriate treatment with potential morbidity. Proper understanding of the clinical presentation and familiarity with the histopathologic features of this disease are therefore imperative. In this paper, we report 2 cases of laryngeal blastomycosis, 1 of which was misdiagnosed as squamous cell carcinoma, clinically and microscopically, with consequent radiotherapy and laryngectomy. In the other case, a clinical diagnosis of glottic squamous cell carcinoma was rendered. However, blastomycosis was identified in a biopsy specimen. We also review cases of isolated laryngeal blastomycosis that have been reported in the English-language literature during the last 80 years. A number of those cases were misdiagnosed clinically and microscopically as squamous cell carcinoma.

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A New Enzyme Immunoassay Specific for Blastomycosis

Cited by 15 publications

References 5 publications

Antigen complex of Coccidioides immitis which elicits a precipitin antibody response in patients

Antigen complex of Coccidioides immitis which elicits a precipitin antibody response in patients

Fulminant blastomycosis with blastomycotic infection of a cerebral glioma

Laryngeal Blastomycosis: A Commonly Missed Diagnosis Report of Two Cases and Review of the Literature

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