A New Enterobacter cloacae Bacteriophage EC151 Encodes the Deazaguanine DNA Modification Pathway and Represents a New Genus within the Siphoviridae Family

Morozova, Vera; Jdeed, Ghadeer; Kozlova, Yuliya; Бабкин, И. В.; Tikunov, Artem; Tikunova, Nina

doi:10.3390/v13071372

Cited by 4 publications

(1 citation statement)

References 30 publications

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“…Instead, phages are also described as polyvalent, as presented by Finney et al (2022), with phages lysing both Enterobacter spp. and Klebsiella aerogenes [ 73 ], or by Addablah et al (2021), with phages infecting E. cloacae and E. coli simultaneously [ 74 ]. Jamal et al (2018) reported the phage MJ2, which was able to inhibit the growth of E. cloacae , Achromobacter xylosoxidans , K. pneumoniae , and P. aeruginosa [ 75 ].…”

Section: Discussionmentioning

confidence: 99%

Two Newly Isolated Enterobacter-Specific Bacteriophages: Biological Properties and Stability Studies

Cieślik

Harhala

Orwat

et al. 2022

Viruses

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In an era of antibiotic therapy crisis caused by spreading antimicrobial resistance, and when recurrent urinary tract infections constitute a serious social and medical problem, the isolation and complex characterization of phages with a potential therapeutic application represents a promising solution. It is an inevitable, and even a necessary direction in the development of current phage research. In this paper, we present two newly isolated myoviruses that show lytic activity against multidrug-resistant clinical isolates of Enterobacter spp. (E. cloacae, E. hormaechei, and E. kobei), the genomes of which belong to a poorly represented phage group. Both phages were classified as part of the Tevenvirinae subfamily (Entb_43 was recognized as Karamvirus and Entb_45 as Kanagawavirus). Phage lytic spectra ranging from 40 to 60% were obtained. The most effective phage-to-bacteria ratios (MOI = 0.01 and MOI = 0.001) for both the phage amplification and their lytic activity against planktonic bacteria were also estimated. Complete adsorption to host cells were obtained after about 20 min for Entb_43 and 10 min for Entb_45. The phage lysates retained their initial titers even during six months of storage at both −70 °C and 4 °C, whereas storage at 37 °C caused a complete loss in their activity. We showed that phages retained their activity after incubation with solutions of silver and copper nanoparticles, which may indicate possible synergistic antibacterial activity. Moreover, a significant reduction in phage titers was observed after incubation with a disinfectant containing octenidinum dihydrochloridum and phenoxyethanol, as well as with 70% ethanol. The observed maintenance of phage activity during incubation in a urine sample, along with other described properties, may suggest a therapeutic potential of phages at the infection site after intravesical administration.

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Section: Discussionmentioning

confidence: 99%

Two Newly Isolated Enterobacter-Specific Bacteriophages: Biological Properties and Stability Studies

Cieślik

Harhala

Orwat

et al. 2022

Viruses

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Isolation, characterization, and application of lytic bacteriophages for controlling Enterobacter cloacae complex (ECC) in pasteurized milk and yogurt

et al. 2023

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Reducing bacterial pathogen contamination not only improves overall global public health but also diminishes food waste and loss. The use of lytic bacteriophages (phages) that infect and kill bacteria could be a beneficial tool for suppressing bacterial growth during dairy products storage time. Four Enterobacter cloacae (E. cloacae) complex isolates which were previously isolated from contaminated dairy products were used to identify lytic phages in wastewater. Phages specific to multi-drug resistant (MDR) E. cloacae complex 6AS1 were isolated from local sewage. Two novel phages vB_EclM-EP1 and vB_EclM-EP2 were identified as myoviral particles and have double-stranded DNA genome. Their host range and lytic capabilities were detected using spot test and efficiency of plating (EOP) against several bacterial isolates. The phages had a latent period of 30 min, and a large burst size of about 100 and 142 PFU/cell for vB_EclM-EP1 and vB_EclM-EP2, respectively. Both phages were viable at pH ranging 5–9 and stable at 70 °C for 60 min. The individual phages and their cocktail preparations (vB_EclM-EP1 and vB_EclM-EP2) reduced and inhibited the growth of E. cloacae complex 6AS1 during challenge test in milk and yogurt samples. These results indicate that the E. cloacae complex-specific phages (vB_EclM-EP1 and vB_EclM-EP2) have a potential application as microbicidal agents in packaged milk and milk derivatives during storage time. In addition, our environment is a rich sources of lytic phages which have potential use in eliminating multidrug-resistant isolates in food industry as well as in biocontrol.

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Transcriptomic Profiling and Characterization of Micrornas in Macrobrachium Rosenbergii Potentially Involved in Immune Response to Enterobacter Cloacae Infection

Zhang

et al. 2023

Preprint

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A New Enterobacter cloacae Bacteriophage EC151 Encodes the Deazaguanine DNA Modification Pathway and Represents a New Genus within the Siphoviridae Family

Cited by 4 publications

References 30 publications

Two Newly Isolated Enterobacter-Specific Bacteriophages: Biological Properties and Stability Studies

Two Newly Isolated Enterobacter-Specific Bacteriophages: Biological Properties and Stability Studies

Isolation, characterization, and application of lytic bacteriophages for controlling Enterobacter cloacae complex (ECC) in pasteurized milk and yogurt

Transcriptomic Profiling and Characterization of Micrornas in Macrobrachium Rosenbergii Potentially Involved in Immune Response to Enterobacter Cloacae Infection

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