A New Enrichment Medium for Certain Salmonellae

A comparison of enrichment media for the isolation of salmonellae from seagull cloacal swabs

“…The carriage of S. typhi in European gulls (Steiniger, 1970) and of subgenus III salmonellae in Australian silver gulls (Western Australia Public Health Department, 1977) has been reported. Although these serotypes are relatively infrequently experienced in the United Kingdom and to our knowledge have not been isolated from seagulls in Scotland, if these serotypes, or indeed S. dublin were being sought from gulls, an alternative enrichment medium such as selenite or tetrathionate broth should be employed, as Rappaport's medium is not suitable for the isolation of these particular serotypes (Rappaport, Konforti & Navon, 1959;Harvey, Price & Hall, 1973;Harvey & Price, 1975).…”

“…After incubation at 37 'C for 20-24 h, each pre-enrichment culture was subcultured, using sterile Pasteur pipettes, into duplicate 10 ml volumes of selenite F and tetrathionate broths at an inoculation ratio of one in ten and to one bottle of each of two formulations of Rappaport's broth, that described by Vassiliadis et al (1970) for incubation at 37 'C (RB 25) and a modification (RB 10) for use at 43 'C (Vassiliadis et al 1976), at an inoculation ratio of one in two hundred. MKTB and SFB were prepared using a commercially available dehydrated base (Oxoid CM 343 and CM 395), whereas the two formulations of Rappaport's broth were prepared in the laboratory from individual constituents (Rappaport, Konforti & Navon, 1959;Vassiliadis et al 1970Vassiliadis et al , 1976.…”

The occurrence and significance to animal health of salmonellas in sewage and sewage sludges

“…and transported to the Institute for Research on Animal Diseases (IRAD) on the day of collection. Isolation of salmonellas Salmonellas were isolated by direct enrichment of 10 ml volumes of sample in two 100 ml volumes of Rappaport broth (Rappaport, Konforti & Navon, 1956) and two 100 ml volumes of selenite brilliant green broth (SBG, Difco Laboratories). In addition salmonellas were isolated by pre-enrichment of 10 ml of sewage in 100 ml of buffered peptone water (Peptone (Difco B1 18) 10 g, sodium chloride 5 g, disodium hydrogen orthophosphate 12 H2O 9 g, potassium dihydrogen orthophosphate 1.5 g, distilled water 11, pH 7.2) for 24 h at 37 0C followed by enrichment of 10 ml of the buffered peptone water in Rappaport broth, SBG, and Muller-Kauffmann tetrathionate broth (Oxoid CM 343).…”

An evaluation of commercially available dehydrated Rappaport-Vassiliadis medium for the isolation of salmonellae from poultry

“…Although Rappaport stressed the importance of Bacto-tryptone in his medium (Rappaport, Konforti & Navon, 1956;Rappaport & Konforti, 1959) recent work has suggested that substitution of other peptones such as soya or mycological peptone may be beneficial (van Schothorst & Renaud, 1983). In comparisons of the efficiency of RV medium containing soya peptone (RV-soya) with that of the standard RV medium, we have demonstrated that RV-soya is a marginally better medium (Fricker & Girdwood, 1985;McGibbon, Quail & Fricker, 1984).…”

“…Since its original description in 1956 (Rappaport, Konforti & Navon, 1956) Rappaport's medium has become widely used for the isolation of salmonellae. Early studies on the use of Rappaport's medium for isolating salmonellae from human faeces were encouraging (Collard & Unwin, 1958;Hooper & Jenkins, 1965; Iveson, Kovacs & Laurie, 1964) although Sen (1964) was disappointed with its performance.…”