A new endangered species of Megaleporinus (Characiformes: Anostomidae) from the Rio de Contas basin, eastern Brazil

Birindelli, José L. O.; Britski, Heraldo A.; Ramírez, Jorge L.

doi:10.1111/jfb.14299

Cited by 7 publications

(5 citation statements)

References 20 publications

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“…Despite underperforming eDNA accuracy regarding species identification, we could still draw important ecological information from the dataset. The high number of Characiformes MOTUs per site falls in line with previous studies in this region (Birindelli et al, 2020;Sales et al, 2021).…”

Section: Discussionsupporting

confidence: 91%

eDNA in a bottleneck: Obstacles to fish metabarcoding studies in megadiverse freshwater systems

et al. 2021

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Section: Discussionsupporting

confidence: 91%

eDNA in a bottleneck: Obstacles to fish metabarcoding studies in megadiverse freshwater systems

et al. 2021

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show abstract

Section: Discussionsupporting

confidence: 90%

eDNA in a bottleneck: obstacles to fish metabarcoding studies in megadiverse freshwater systems

Jackman

Benvenuto

Coscia

et al. 2021

Preprint

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The current capacity of environmental DNA (eDNA) to provide accurate insights into the biodiversity of megadiverse regions (e.g., the Neotropics) requires further evaluation to ensure its reliability for long-term monitoring. In this study, we first evaluated the taxonomic resolution capabilities of a short fragment from the 12S rRNA gene widely used in fish eDNA metabarcoding studies, and then compared eDNA metabarcoding data from water samples with traditional sampling using nets. For the taxonomic discriminatory power analysis, we used a specifically curated reference dataset consisting of 373 sequences from 264 neotropical fish species (including 47 newly generated sequences) to perform a genetic distance-based analysis of the amplicons targeted by the MiFish primer set. We obtained an optimum delimitation threshold value of 0.5% due to lowest cumulative errors. The barcoding gap analysis revealed only a 50.38% success rate in species recovery (133/264), highlighting a poor taxonomic resolution from the targeted amplicon. To evaluate the empirical performance of this amplicon for biomonitoring, we assessed fish biodiversity using eDNA metabarcoding from water samples collected from the Amazon (Adolpho Ducke Forest Reserve and two additional locations outside the Reserve). From a total of 84 identified Molecular Operational Taxonomic Units (MOTUs), only four could be assigned to species level using a fixed threshold. Measures of α-diversity analyses within the Reserve showed similar patterns in each site between the number of MOTUs (eDNA dataset) and species (netting data) found. However, β-diversity revealed contrasting patterns between the methods. We therefore suggest that a new approach is needed, underpinned by sound taxonomic knowledge, and a more thorough evaluation of better molecular identification procedures such as multi-marker metabarcoding approaches and tailor-made (i.e., order-specific) taxonomic delimitation thresholds.

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“…The teeth preserve at their lateral edges a contact surface for other premaxillary teeth. Among the Anostomidae, premaxillary symphyseal teeth with a massive unicuspid incisiform shape are characteristics of Megaleporinus, of which eleven species are recognized (Ramirez et al 2017;Birindelli et al 2020). In contrast, Abramites, Anostomoides, and Leporinus have compressed teeth with a mesial ridge and usually a single outstanding cusp, often accompanied by smaller lateral cusps in juveniles (see Birindelli et al 2013;Assega and Birindelli 2019).…”

Section: General Description Comparisons and Remarksmentioning

confidence: 99%

A Pliocene–Pleistocene continental biota from Venezuela

Carrillo-Briceño

Sánchez²,

Scheyer

et al. 2021

Swiss J Palaeontol

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The Pliocene–Pleistocene transition in the Neotropics is poorly understood despite the major climatic changes that occurred at the onset of the Quaternary. The San Gregorio Formation, the younger unit of the Urumaco Sequence, preserves a fauna that documents this critical transition. We report stingrays, freshwater bony fishes, amphibians, crocodiles, lizards, snakes, aquatic and terrestrial turtles, and mammals. A total of 49 taxa are reported from the Vergel Member (late Pliocene) and nine taxa from the Cocuiza Member (Early Pleistocene), with 28 and 18 taxa reported for the first time in the Urumaco sequence and Venezuela, respectively. Our findings include the first fossil record of the freshwater fishes Megaleporinus, Schizodon, Amblydoras, Scorpiodoras, and the pipesnake Anilius scytale, all from Pliocene strata. The late Pliocene and Early Pleistocene ages proposed here for the Vergel and Cocuiza members, respectively, are supported by their stratigraphic position, palynology, nannoplankton, and 86Sr/88Sr dating. Mammals from the Vergel Member are associated with the first major pulse of the Great American Biotic Interchange. In contrast to the dry conditions prevailing today, the San Gregorio Formation documents mixed open grassland/forest areas surrounding permanent freshwater systems, following the isolation of the northern South American basin from western Amazonia. These findings support the hypothesis that range contraction of many taxa to their current distribution in northern South America occurred rapidly during at least the last 1.5 million years.

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A new endangered species of Megaleporinus (Characiformes: Anostomidae) from the Rio de Contas basin, eastern Brazil

Cited by 7 publications

References 20 publications

eDNA in a bottleneck: Obstacles to fish metabarcoding studies in megadiverse freshwater systems

eDNA in a bottleneck: Obstacles to fish metabarcoding studies in megadiverse freshwater systems

eDNA in a bottleneck: obstacles to fish metabarcoding studies in megadiverse freshwater systems

A Pliocene–Pleistocene continental biota from Venezuela

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