A new disposable electrode for electrochemical study of leukemia K562 cells and anticancer drug sensitivity test

Yu, Chunmei; Zhu, Zhenkun; Wang, Li; Wang, Qiuhong; Bao, Ning; Gu, Hongchen

doi:10.1016/j.bios.2013.09.044

Cited by 46 publications

(21 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a study of Chunmei Yu et al ‏. 25 by modified electrode on K562 cancer cell line treated with arsenic trioxide and cyclophosphamide through CV and DPV exhibited that increasing the drug concentration induced the decrease of electrochemical responses significantly. In another works by João G. Pacheco et al‏.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Flavonoid Derivative and Doxorubicin Effects in Lung Cancer Cells (A549) Using Differential Pulse Voltammetry Method

et al. 2018

View full text Add to dashboard Cite

Purpose: Electrochemical measurements have prompted the progress as a consequence of their affectability, cost-affectivity and comparatively short examination time. The aim of this study was the fast evaluation of the effect of chemotherapy compounds on the viability of lung cancer cells (A549) via electrochemical methods.Methods: Cyclic voltammetry (CV) was used as a primary method to distinguish between electrochemical behavior of normal and lung cancer cells. Differential pulse voltammetry (DPV) was employed as a complementary analyses method for the impact of doxorubicin (DOX) and Flavonoid modified drug (FMD) (US patent Application number: 62548886) on Lung cancer cells.Results: Only one oxidative peak, at approximately -0.15 V was detected through DPV method in cancer cell line. While a significant distinguish was not seen in CV. The current intensity (I) was decreased in cancer cells with increasing the DOX and FMD levels (t=99.027, α=0.05, P=0.0000), (t=135.513, α=0.05, P=0.0000), respectively.Conclusion: The movement of cancerous cells towards death through chemotherapy drugs such as DOX and FMD can make distinct and significant changes in the electrochemical behaviors of those cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Evaluation of Flavonoid Derivative and Doxorubicin Effects in Lung Cancer Cells (A549) Using Differential Pulse Voltammetry Method

et al. 2018

View full text Add to dashboard Cite

show abstract

“…A key example was reported by Yu et al [52] which home-made an analytical device using indium tin oxide (ITO) as working electrode and paper as electrolytic cell to perform electrochemical study of leukaemia K562 cells, to evaluate the cytotoxicity of anticancer drugs. In this case, an ITO glass was combined with punched adhesive tape and filter paper to measure guanine oxidation in cells dropped on a disposable electrode treated or untreated with arsenic trioxide and cyclophosphamide.…”

Section: Sensorsmentioning

confidence: 99%

Past, Present and Future Challenges of Biosensors and Bioanalytical Tools in Analytical Chemistry: A Tribute to Professor Marco Mascini

Arduini¹,

Cinti²,

Viviana³

et al. 2017

Comprehensive Analytical Chemistry

View full text Add to dashboard Cite

“…In recent years, particular interest has been focused on methods analyzing the electrochemical behavior of cells, [8][9][10] which are rapid, highly sensitive, and inexpensive compared with conventional morphological methods. Electrochemical methods such as electrochemical impedance spectroscopy, 11,12 cyclic voltammetry, 13,14 and electric cell-substrate impedance sensing 15,16 can effectively determine cell viability and be used as in vitro sensitivity tests for toxins. Efforts launched in this area have been focused mostly on constructing nanomaterial-modied electrodes to obtain better electrochemical responses 17,18 and on cell immobilization.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical behavior of a pheochromocytoma cell suspension and the effect of acrylamide on the voltammetric response

Jiang

Sun

et al. 2015

Anal. Methods

View full text Add to dashboard Cite

The electrochemical behavior of electroactive species in a pheochromocytoma cell (PC-12) suspension was studied to establish a simple and rapid measurement method to obtain strong and direct electrochemical responses that objectively reflect cell viability. Two electrochemical signals of the PC-12 suspension, caused by the oxidation of guanine, xanthine, adenine and hypoxanthine, were simultaneously detected by using a glass carbon electrode modified with a multi-walled carbon nanotube-graphene nanosheet hybrid film. The presence of the four purines in the PC-12 cell cytoplasm was verified by HPLC assay using a DAD system and LC-MS analysis. Additionally, a linear relationship between the peak currents of purines in the cell suspension and culture time was found, which is in accord with the cell counts. The cytotoxic effect of acrylamide (0.1 mM to 10 mM) on cells was evaluated by differential pulse voltammetry (DPV) analysis, which detected a decrease in the voltammetric response of purines in the cytoplasm, in agreement with that obtained by the MTT test and with morphological analysis. Therefore, the voltammetric response of the ultrasonicated PC-12 cell suspension could be used to monitor cell growth and to evaluate the effects of potential toxic hazards on cells, and in drug screening.

show abstract

A new disposable electrode for electrochemical study of leukemia K562 cells and anticancer drug sensitivity test

Cited by 46 publications

References 44 publications

Evaluation of Flavonoid Derivative and Doxorubicin Effects in Lung Cancer Cells (A549) Using Differential Pulse Voltammetry Method

Evaluation of Flavonoid Derivative and Doxorubicin Effects in Lung Cancer Cells (A549) Using Differential Pulse Voltammetry Method

Past, Present and Future Challenges of Biosensors and Bioanalytical Tools in Analytical Chemistry: A Tribute to Professor Marco Mascini

Electrochemical behavior of a pheochromocytoma cell suspension and the effect of acrylamide on the voltammetric response

Contact Info

Product

Resources

About