A new diabetic strain of rat (WBN/Kob)

Tsuchitani, Minoru; Saegusa, Tadashi; Narama, Isao; Nishikawa, Tetsu; Gonda, Tatsuo

doi:10.1258/002367785780893575

Cited by 95 publications

(52 citation statements)

References 8 publications

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“…In WBN/kob rat, myelinopathy and axonopathy with myelinated fiber loss caused by subsequent chronic pancreatitis and hyperglycemia have also been reported [16,19,28,33]. We consider that continuous hyperglycemia or hyperinsulinemia from a young age in TSOD mice might affect the peripheral nerves metabolically [34].…”

Section: Discussionmentioning

confidence: 84%

Diabetic Complications in a New Animal Model (TSOD mouse) of Spontaneous NIDDM with Obesity

et al. 2005

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Section: Discussionmentioning

confidence: 84%

Diabetic Complications in a New Animal Model (TSOD mouse) of Spontaneous NIDDM with Obesity

et al. 2005

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“…These findings suggest a dysfunction of the intracellular Ca 2+ handling system in the spontaneously diabetic heart, which is likely to include impaired Ca 2+ sequestration and/or extrusion. The WBN/Kob (Wistar Bonn/Kobori) rat is an inbred strain of Wistar rat in which the male rats develop diabetes between the ages of 9 and 15 months [41,42,44]. Tsuchida et al [41] demonstrated abnormal ventricular function in 8 to 19-month-old male WBN/Kob rats, and showed that these rats resemble the NIDDM model, to some extent, in terms of the slow progression of pathophysiological change and a long span without insulin administration.…”

Section: Discussionmentioning

confidence: 99%

“…This suggests possible differences of electrophysiological and mechanical properties between STZ-treated diabetic rats and spontaneously diabetic rats. It has been reported that male WBN/Kob rats spontaneously develop DM between the ages of 9 and 15 months [41,42,44]. However, acute pancreatitis and the pancreatic fibrosis were found even in 2-3 month-old WBN/Kob rats [18,43].…”

Section: Introductionmentioning

confidence: 99%

Postrest Contraction in the Ventricular Papillary Muscle of Spontaneously Diabetic WBN/Kob Rat.

et al. 2001

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Abstract:In the present study, we investigated the characteristics of the postrest contraction (PRC) in chronic diabetic ventricular muscle. We used WBN/Kob rats of 7-8 weeks as the spontaneously diabetic animal and Wistar rats of 7-8 weeks as the control. We found: (1) No significant differences were seen in the amplitude, the contracting speed, and the relaxing speed of electrically stimulated twitch tension between control and WBN/ Kob rats. In addition, the relationship between amplitude of twitch tension and stimulus cycle lengths (0.2-5 sec) was very similar in both animals. (2) The ratios of the first twitch tension (T 1 ) of PRC with various rest intervals (5-600 sec) to the steady-state tension (T ss ) were significantly smaller in the diabetic rats than in the controls. (3) When the preparation was stimulated at shorter cycle lengths, the recovery process of PRC was separated into at least two components (fast and slow components). In the diabetic rats, the time constant (τ) of both components was significantly longer than in controls. (4) After caffeine (10 -3 M) treatment, τ of the fast component in the control rats became longer, whereas it remained unchanged in diabetic rats. These findings suggest a dysfunction of the intracellular calcium handling system in spontaneously diabetic heart that is likely to include impaired calcium sequestration and/or extrusion.

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“…The spontaneous diabetic syndrome has been reported in aged males of the WBN/Kob rat strain [15][16][17][18][19][20][21][22]. These animals show reduced glucose tolerance at 5 months of age and develop the spontaneous diabetic syndrome around 9 months of age [18].…”

Section: Discussionmentioning

confidence: 99%

Changes in the immunoreactivity of protein gene product (PGP) 9.5 in the cochlea of spontaneously diabetic WBN/Kob rats

1997

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In our previous study, hearing impairment of spontaneously diabetic WBN/Kob rats was first detected as an elevation of the hearing threshold in the auditory brainstem response in animals expressing glucose intolerance, then as a decrease in the number of spiral ganglion cells in the cochlea, and finally as oedematous changes in the stria vascularis [1]. Atrophy of the spiral ganglion was also reported as the most common pathological change in the cochlea of diabetic patients [2]. On the other hand, functional changes in the spiral ganglion cells caused by diabetes mellitus remain to be examined.Protein gene product (PGP) 9.5 is a ubiquitin carboxyl-terminal hydrolase [3] prevalent in cells of neural origin [4][5][6]. Although the functional significance of PGP 9.5 is still unclear, this protein may play an essential role in the ubiquitin-related metabolism in nervous tissues. Recently, PGP 9.5 has been increasingly used as a convenient neural marker in neurophysiological studies [7][8][9][10][11][12]. In the present study, therefore, we examined the immunoreactivity for PGP 9.5 in the spiral ganglion cells of the cochlea in prediabetic and diabetic WBN/Kob rats by an immunohistochemical method with computer-assisted image analysis and compared the results with those obtained from normal age-matched Wistar rats in order to reveal the functional changes in the spiral ganglion cells from a quantitative point of view. Diabetologia (1997) 40: 173-178 Changes in the immunoreactivity of protein gene product (PGP) 9.5 in the cochlea of spontaneously diabetic WBN/Kob rats Summary The mechanism of hearing impairment due to diabetes mellitus was examined in relation to changes in the level of the immunoreaction for the protein gene product 9.5 in the cochlea of spontaneously diabetic WBN/Kob rats. At 7 months (33 weeks) of age, when half of the males of this strain manifest diabetes, male WBN/Kob rats were divided into two groups as follows: one group consisted of prediabetic animals showing slightly decreased tolerance to glucose with a normal plasma concentration of glucose, normal urinary excretion of glucose, and functional hearing impairment (assessed in terms of elevation of hearing threshold). The second group consisted of diabetic animals with glucose intolerance, high plasma glucose level, polyuria, urinary glucose excretion, and more apparent elevation of hearing threshold. According to morphometric analysis of the spiral ganglion, the number of ganglion cells was significantly smaller in both the prediabetic and the diabetic animals than in the age-matched control Wistar rats. The staining intensity for protein gene product 9.5 was increased in some spiral ganglion cells of diabetic animals, but decreased in others according to quantitative immunohistochemical analysis. On the other hand, the immunoreactivity for protein gene product 9.5 was similar in the prediabetic animals to that in the control Wistar rats. These results suggest that numerical and immunohistochemical changes in the spiral ganglion cells r...

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A new diabetic strain of rat (WBN/Kob)

Cited by 95 publications

References 8 publications

Diabetic Complications in a New Animal Model (TSOD mouse) of Spontaneous NIDDM with Obesity

Diabetic Complications in a New Animal Model (TSOD mouse) of Spontaneous NIDDM with Obesity

Postrest Contraction in the Ventricular Papillary Muscle of Spontaneously Diabetic WBN/Kob Rat.

Changes in the immunoreactivity of protein gene product (PGP) 9.5 in the cochlea of spontaneously diabetic WBN/Kob rats

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