A new design intended to relate high pressure treatment to yeast cell mass transfer

Perrier-Cornet, Jean-Marie; Marechal, Pierre-André; Gervais, Patrick

doi:10.1016/0168-1656(95)00052-r

Cited by 59 publications

(39 citation statements)

References 15 publications

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“…The volume of nonviable cells was found to be 65% after the holding time of 15 min. -Cornet et al (1995) tally validated with yeast cell volume reduction data from Perrier-Cornet et al (1995). Instead of using a volume loss equation as was done in the study of Smith et al (1998), a reduced form of the Cauchy equation of motion represented the mechanical behaviour of the yeast cell.…”

Section: The Mechanisms Of Cellular Inactivationmentioning

confidence: 99%

Recent Advances in the Use of High Pressure as an Effective Processing Technique in the Food Industry

Norton

Sun

2007

Food Bioprocess Technol

364

172

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High pressure processing is a food processing method which has shown great potential in the food industry. Similar to heat treatment, high pressure processing inactivates microorganisms, denatures proteins and extends the shelf life of food products. But in the meantime, unlike heat treatments, high pressure treatment can also maintain the quality of fresh foods, with little effects on flavour and nutritional value. Furthermore, the technique is independent of the size, shape or composition of products. In this paper, many aspects associated with applying high pressure as a processing method in the food industry are reviewed, including operating principles, effects on food quality and safety and most recent commercial and research applications. It is hoped that this review will promote more widespread applications of the technology to the food industry.

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Section: The Mechanisms Of Cellular Inactivationmentioning

confidence: 99%

Recent Advances in the Use of High Pressure as an Effective Processing Technique in the Food Industry

Norton

Sun

2007

Food Bioprocess Technol

364

172

View full text Add to dashboard Cite

show abstract

“…Several researchers used Diamond-Anvil-Cells, which produce transmitted light images, but fluorescence observations are still lacking (Bassett et al, 1993;Chou et al, 1998;Couzin, 2002;Maeda and Koizumi, 1996;Sharma et al, 2002;Yayanos, 2002). The high-pressure in situ observation systems in combination with common microscopes meets the same fate (Besch and Hogan, 1996;Butz, 1987;Deguchi and Tsujii, 2002;Hogan et al, 1981;Koyama et al, 2001;Pagliaro et al, 1995;Perrier-Cornet et al, 1995;Reck et al, 1998). The first attempt to work with fluorescent dyes and high hydrostatic pressure is described by a fixation system under high pressure (Ishii et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Many attempts were carried out to obtain microscopic pictures under high pressure, with a sufficient quality and a high resolution (Besch and Hogan, 1996;Butz, 1987;Deguchi and Tsujii, 2002;Hogan et al, 1981;Pagliaro et al, 1995;Perrier-Cornet et al, 1995;Reck et al, 1998). In 2001, Koyama described a practicable microscopic system withstanding pressures up to 100 MPa.…”

Section: Introductionmentioning

confidence: 99%

Microscopy under pressure—An optical chamber system for fluorescence microscopic analysis of living cells under high hydrostatic pressure

Frey

Hartmann

Herrmann

et al. 2006

Microscopy Res & Technique

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High hydrostatic pressure (HHP) becomes more and more interesting for life science research, since it can be employed to inactivate various cells. To directly monitor "cells under pressure," the development of an optical high-pressure chamber is required. Therefore, an optical pressure chamber that can be used for up to 300 MPa was constructed. This chamber has already been described as a tool for in situ observation of dynamic changes of microscopic structures in bright field as well as phase contrast. In combination with an inverted microscope, we obtained brilliant microscopic color pictures with an optical resolution more than 0.56 microm. Here, we demonstrate the capabilities of the HHP cell, in combination with epifluorescence microscopy. Using a nonadherent human B-cell line (Raji, ATCC CCL 86), stained with the fluorescent dyes propidium iodide, Hoechst 33342, or dihexyloxacarbocyanine iodide, we were able to show that the system is suitable to perform fluorescence microscopic analyses, with pressures up to 300 MPa, with viable mammalian cells.

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“…Stop flow cells have been tested by Zalewski and Buchholz [40] for yeast cells and by Treskatis et al [41] for S. tendae pellets, combined with an inverted microscope. Specific cells have been designed to investigate the effect of osmotic shifts [42] and high pressure [43] on yeast cells. As in any automated sampling system for bioprocesses, there are risks of tubing clogging and fouling of the transparent optical surfaces.…”

Section: Mode Of Operationmentioning

confidence: 99%

Biomass Quantification by Image Analysis

Pons

Vivier

1999

Bioanalysis and Biosensors for Bioprocess Monitoring

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Microbiologists have always rely on microscopy to examine microorganisms. When microscopy, either optical or electron-based, is coupled to quantitative image analysis, the spectrum of potential applications is widened: counting, sizing, shape characterization, physiology assessment, analysis of visual texture, motility studies are now easily available for obtaining information on biomass. In this chapter the main tools used for cell visualization as well as the basic steps of image treatment are presented. General shape descriptors can be used to characterize the cell morphology, but special descriptors have been defined for filamentous microorganisms. Physiology assessment is often based on the use of fluorescent dyes. The quantitative analysis of visual texture is still limited in bioengineering but the characterization of the surface of microbial colonies may open new prospects, especially for cultures on solid substrates. In many occasions, the number of parameters extracted from images is so large that data-mining tools, such as Principal Components Analysis, are useful for summarizing the key pieces of information.

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A new design intended to relate high pressure treatment to yeast cell mass transfer

Cited by 59 publications

References 15 publications

Recent Advances in the Use of High Pressure as an Effective Processing Technique in the Food Industry

Recent Advances in the Use of High Pressure as an Effective Processing Technique in the Food Industry

Microscopy under pressure—An optical chamber system for fluorescence microscopic analysis of living cells under high hydrostatic pressure

Biomass Quantification by Image Analysis

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