A New DBS Card with Spot Sizes Independent of The Hematocrit Value of Blood

Mengerink, Ynze; Mommers, John; Qiu, Jun; Mengerink, Josh G. M.; Steijger, O.M.; Honing, Maarten

doi:10.4155/bio.15.133

Cited by 22 publications

(15 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, Mengerink et al presented the use of a hydrophilically coated woven polyester fiber as a Hct-independent substrate for DBS analysis, named Qyntest cards (Qynion, Geelen, The Netherlands). It was demonstrated that the cards generate spots with a Hct-independent size within a Hct range of 20 to 70%, allowing to use partial spot analysis without the Hct volume bias [55]. A clear drawback here is that this is a completely distinct material, which also may be more expensive to produce, as compared to conventional cellulose-based cards.…”

Section: Minimizing the Hct Issuementioning

confidence: 99%

Is the hematocrit still an issue in quantitative dried blood spot analysis?

Velghe

Delahaye

Stove

2019

Journal of Pharmaceutical and Biomedical Analysis

158

107

View full text Add to dashboard Cite

Hematocrit-related issues remain a major barrier for (regulatory) acceptance of the classical dried blood spot (DBS) analysis in the bioanalytical and clinical field. Lately, many attempts to cope with these issues have been made. Throughout this review, an overview is provided on new strategies that try to cope with this hematocrit effect (going from avoiding to minimizing), on methods estimating a DBS volume, and on methods estimating or measuring the hematocrit of a DBS. Although many successful strategies have been put forward, a combination of different technologies still provides the most complete solution. Therefore, further efforts and the availability of a straightforward guideline for analytical and clinical method validation should help to overcome the hurdles still associated with DBS sampling.

show abstract

Section: Minimizing the Hct Issuementioning

confidence: 99%

Is the hematocrit still an issue in quantitative dried blood spot analysis?

Velghe

Delahaye

Stove

2019

Journal of Pharmaceutical and Biomedical Analysis

158

107

View full text Add to dashboard Cite

show abstract

“…The small volume decreases sensitivity and accuracy of quantitation, while non-uniform blood distribution across a DBS and changes in hematocrit levels can arise due to collection volume uncertainty. Non-homogeneity across a DBS sample have been addressed with a variety of approaches, including whole spot analysis and volumetric sampling 30 – 37 , 47 , 48 . We have developed a specially designed volumetric blood collection device which yields a sufficient quantity of sample to test any number of proteins in a multi-plex immunoassay, with bleeding capacity being the most significant limiting factor of sample volume.…”

Section: Discussionmentioning

confidence: 99%

Dried blood sample analysis by antibody array across the total testing process

Whittaker

Mao

Lin

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Dried blood samples (DBSs) have many advantages; yet, impediments have limited the clinical utilization of DBSs. We developed a novel volumetric sampling device that collects a precise volume of blood, which overcomes the heterogeneity and hematocrit issues commonly encountered in a traditional DBS card collection as well as allowing for more efficient extraction and processing procedures and thus, more efficient quantitation, by using the entire sample. We also provided a thorough procedure validation using this volumetric DBS collection device with an established quantitative proteomics analysis method, and then analyzed 1000 proteins using this approach in DBSs concomitantly with serum for future consideration of utility in clinical applications. Our data provide a first step in the establishment of a DBS database for the broad application of this sample type for widespread use in clinical proteomic and other analyses applications.

show abstract

“…[31][32][33][34] Moreover, some types of collection devices have been reported to be less affected by the HT effect and may help to overcome this issue. 35,36 Additionally, chitosan and alginate foams have been proposed as collection substrates to help increase analyte recovery, as they dissolve during sample extraction. 37 Although most DBS-based bioanalytical methods use regular, cellulose-based, untreated filter paper (cards), for certain applications it may be valuable to evaluate the use of pretreated or non-cellulose-based alternatives.…”

Section: A C C E P T E Dmentioning

confidence: 99%