A New Copper (Ii)-Imidazole Derivative Effectively Inhibits Replication of Denv-2 in Vero Cell

Abstract

Background: Dengue is a kind of infectious disease that was distributed in the tropical and subtropical areas. To date, there is no clinically approved dengue vaccine or antiviral for humans, even though there have been great efforts towards this end. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the complex compounds, copper(II)-imidazole derivatives are of interest because of their biological and medicinal benefits. Materials and Methods: In the present study, antiviral activity of [Cu(2,4,5-triphenylimidazole) 2 ] n , was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cell using focus forming unit reduction assay and quantitative ELISA. Results: [Cu(2,4,5-triphenylimidazole) 2 ] n inhibited DENV-2 replication in Vero cells with IC 50 = 2.3 μg/ml and SI= 19.42 when cells were treated 2 days after virus infection, whereas its CC 50 for cytotoxicity to Vero cells was 44.174 μg/ml. Conclusion: The compound has high anti-DENV2 activity, less toxicity, and a high possibility to be considered a drug candidate.

The influence of metal on the performance of 2,4,5-triphenylimidazole as an inhibitor of dengue virus replication

“…Subsequently, the plates were incubated with 5% CO2 at 37°C for 24 hours. Finally, the plate was verified using DENV antibody (4G2) for quantitative ELISA using Microplate Reader with a wavelength of 415 nm (Sucipto et al, 2018).…”

“…Then the mixture was vortexed until homogeneous which is then incubated 30-60 minutes. After that, it is analyzed with a microplate reader with a wavelength of 450 nm and a reference wavelength of 650 nm (Sucipto et al, 2018).…”

“…Besides, phenyl-imidazole has a great potential as drug candidate to treatment of a variety of viral diseases, phenyl rings in imidazole presence of electron withdrawing groups improved the biological activity (Sharma et al, 2009). (Sucipto et al, 2018)] * Unpublished Data (CC50), Inhibition Concentration (IC50), and Selectivity Index (SI). The anti-dengue activity of complex compounds was also compared with the ligand TPI.…”

“…Until now, there is no vaccine or anti-viral for humans that could overcome this disease. Imidazole and their derivatives are proven has potency against dengue virus infection in cell lines (Sucipto et al, 2018;Sucipto et al, 2017). On the other hand, the presence of metal on the structure of organic ligand is proven could improve their biological activity (Geersing et al, 2018) The complex compound is formed as a result of metal and organic compound reaction.…”

Effect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell

“…The compound exhibited adsorption inhibitory activity against DENV-2 at IC 50 = 2.3 μg/ml. 12 The Zn 2+ ion can be used as an antiviral candidate. 13 Zn 2+ ions can change the activity of various transcription factors and thus, patterns of cellular and viral gene expression.…”