“…in a low yield of 0.00061% . Although methoxyeugenol could be chemically synthesized from syringate (4-hydroxy-2,6-dimethoxybenzoic acid), the method has suffered from the high cost and pollution risk by using synthetic material syringate and environmentally hazardous reagent hydrochloric acid. − With the increasing demand for methoxyeugenol, finding a new, sustainable, and practical method for producing this multifunctional compound is necessary. In recent years, synthetic biology has been successfully employed to produce plant bioactive compounds such as paclitaxel, tanshinone, and artemisinin, which inspires us to elucidate the biosynthetic pathway of methoxyeugenol and solve the problem of its shortage by biosynthesis. − In the biosynthetic pathway from coniferyl alcohol to eugenol, coniferyl alcohol is acylated to generate an esterification product, producing eugenol via eugenol synthase .…”