A New Concept of Plant In Vitro Cultivation Liquid Medium: Temporary Immersion

Teisson, Claude; Alvard, Daniel

doi:10.1007/978-94-011-0307-7_12

Cited by 105 publications

(66 citation statements)

References 7 publications

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“…According to Teisson and Alvard (1995) with 1-litre temporary immersion bioreactors, 15 min' immersion every 6 h led to successive development and germination of coffee embryos, whereas for an identical culture medium, immersions of 1 min every 24 h halted embryo development and stimulated the production of adventive embryos.…”

Section: Somatic Embryo Mass Regeneration In Bioreactorsmentioning

confidence: 99%

Bioreactors in coffee micropropagation

Etienne

Déchamp

Barry-Etienne

et al. 2006

Braz. J. Plant Physiol.

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In coffee, bioreactors are the most promising way for scaling-up micropropagation processes, particularly somatic embryogenesis. The availability of an efficient somatic embryogenesis process would allow the rapid mass production of heterozygous materials such as selected Coffea canephora clones and F 1 Arabica hybrid varieties. For the last fifteen years, bioreactors (mechanically or pneumatically agitated bioreactors, temporary immersion bioreactors) have mostly been used on coffee to optimize the mass regeneration of somatic embryos from embryogenic tissues. This review presents the main results, obtained with several bioreactor models, concerning the different steps of the micropropagation process : i) the multiplication of embryogenic tissues, ii) the somatic embryo mass regeneration and iii) the production of pre-germinated embryos and plantlets in bioreactors. The literature shows that scaling-up can be successful, since very efficient embryo production has been achieved for both C. arabica and C. canephora. Moreover, it was proven that the pre-germinated coffee embryos -i.e. embryonic axis elongation (10-12 mm), root tip formation, cotyledon expansion and greening -obtained in temporary immersion bioreactors were photoautotrophic and able to regenerate vigorous plantlets after sowing under nursery conditions. The feasibility to apply the bioreactor technology in an industrial micropropagation procedure is also discussed in the particular socio−economic context of coffee growing.Key words: Coffea, breeding, liquid medium, mass propagation, somatic embryogenesis, temporary immersion.Bioreatores na micropropagação do café: Em café, o uso de bioreatores é a mais promissora maneira de aumentar o processo de micropropagação, particularmente a embriogênese somática. A disponibilidade de um eficiente processo de embriogênese somática poderia aumentar a rápida produção em massa de materiais heterozigotos, tais como clones selecionados de Coffea canephora clones e variedades híbridas F 1 de Coffea arabica. Nos últimos 15 anos, bioreatores (bioreatores mecanicamente ou pneumaticamente agitados, bioreatores de imersão temporária) têm sido predominantemente usados em café para otimizar a regeneração em massa de embriões somáticos, a partir de tecidos embriogênicos. Esta revisão apresenta os principais resultados obtidos com vários modelos de bioreatores, no que diz respeito aos vários passos do processo de micropropagação: i) a multiplicação de tecidos embriogênicos, ii) a regeneração em massa de embriões somáticos e iii) a produção de embriões pré-germinados e plântulas nos bioreatores. A literatura mostra que o escalonamento do processo de micropropagação pode ser útil, desde que uma produção muito eficiente de embriões seja atingida para C. arabica e C. canephora. Além disso, foi demonstrado que embriões pré-germinados de café -i.e. com alongamento do eixo embrionário (10-12 mm), formação de ponta de radícula, expansão do cotilédone e esverdeamento -obtidos em bioreatores de imersão temporária eram fotoautotrófico...

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Section: Somatic Embryo Mass Regeneration In Bioreactorsmentioning

confidence: 99%

Bioreactors in coffee micropropagation

Etienne

Déchamp

Barry-Etienne

et al. 2006

Braz. J. Plant Physiol.

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“…The results from this study showed that 30 min immersion every 12 h and 60 min immersion every 24 h induced vitrification and pollution rate in shoot tips 16.6% and 19%, 33% and 37%, respectively. There are distinct decreases in pollution rate (8.3%) in TIBS (10 min every 6 h) compared with that (more than 50%) in conventional semi-solid and liquid culture, which may due to that TIBS causing gentle ventilation of the air in the bioreactor allows medium components sufficient and the sequential medium changing [19][20][21]. However, changing immersion frequencies from every 4 h to 8h, vitrification did not occur.…”

Section: High Efficiency Of Propagation For Sagittaria Sagittifolia Umentioning

confidence: 91%

High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

Gao¹,

Lin²,

Luo³

et al. 2016

JAST-A

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Abstract:Although several studies have reported on the propagation of the Sagittaria sagittifolia to date, none of these methods have efficiently achieved the mass production of these plants. The present study aimed to investigate the propagation and growth of S. sagittifolia using a temporary immersion bioreactor system (TIBS) compared with conventional semi-solid and liquid culture. The effect of different immersion frequencies and immersion times together with supplementation of various plant growth regulators to the Murashige and Skoog (MS) medium was evaluated on shoot proliferation and plant growth. The results showed that the higher immersion frequency (every 6 h) and shorter immersion time (3 min and 10 min) in medium containing 4 mg/L BA and 0.1 mg/L NAA produced the highest multiplication rate (23), which are significantly higher than conventional semi-solid (3.6) and liquid (4.5) method, and the best plant growth parameter. While, the lower immersion frequency and longer immersion time (30 min every 12 h and 60 min every 24 h) induced vitrification and pollution rate in shoot tips 16.6% and 19%, 42% and 37%, respectively. There is distinct decrease in pollution rate (8.3%) in TIBS (10 min every 6 h) compared with the conventional semi-solid and liquid cultures. Medium containing 4 mg/L BA and 0.5 mg/L NAA using 10 min immersion every 6 h showed satisfaction at the rooting stage, with high shoot proliferation rate (21.6), 100% rooting and 94% plant survival. Therefore, applying TIBS in S. sagittifolia is an efficient method for scaling up the production of plantlets with high quality seedlings.

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“…The temporal immersion system, type Rita ® [developed by Teisson and Alvard (1995) with 200 ml culture medium for germination], is used for germination of somatic embryos according to López (2006). The immersion frequency was three times daily, during 1 min each according to Escalant et al (1994).…”

Section: Germination Of Somatic Embryos In Temporal Immersion Systemmentioning

confidence: 99%

Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.)

López

Rayas

Santos

et al. 2016

Biotechnologies for Plant Mutation Breeding

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Bananas and plantains (Musa spp.) are among the world's most important crops and are considered as a poor man's crop in tropical and subtropical countries. For this reason, they play a very important role in tropical regions' food security. Several pathogens throughout the tropical areas of the world have become a threat for production, including "black sigatoka" (Mycosphaerella fijiensis Morelet) which is the most damaging disease for Musa plantations in Cuba. It is present in all areas of the country, and it replaced "sigatoka disease" (Mycosphaerella musicola) as the main threat. Chemical control and cultural practices reduce damages, but they are not solving the problem. Genetic variations are the basic tools to develop new cultivars with better traits, such as tolerance against various environmental stresses, resistance against pests and diseases, and improved yield and quality. Tissue culture techniques offer the opportunity for variation induction, handling of large plant populations using established selection methods, and rapidly cloning selected variants. All these strategies can increase the efficiency of mutagenic treatments and subsequent screening of mutant materials. Somatic embryogenesis (SE) is also an excellent system for clonal propagation and mutation induction. The present chapter describes a protocol on how to use embryogenic cell suspensions (ECS) in plantain (Musa spp.) using both in vitro gamma irradiation and plant regeneration in order to achieve genetic improvement. The process involves a series of steps to properly select ECS for irradiation and the posttreatment handling for plant regeneration as well as mutant selection during acclimatization phase and under field conditions.

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A New Concept of Plant In Vitro Cultivation Liquid Medium: Temporary Immersion

Cited by 105 publications

References 7 publications

Bioreactors in coffee micropropagation

Bioreactors in coffee micropropagation

High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.)

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