A new closterovirus in Alcea rosea identified by small RNA deep sequencing

Wang, Defu; Cui, Liyan; Yu, Peixia; Yonghong, Qi; Wang, Baoxia; Niu, Yanbing

doi:10.1007/s42161-019-00369-5

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…Interestingly, highly abundant 22-nt-class small RNAs, potentially derived from the 3′-terminal end of the WhCV1 negative-strand genome RNA, were found ( Figure 5 C and Supplementary Figure S4 ). This may be due to the formation of highly structured RNA in the terminal end of the genome, but the genomes of other closterovirids are not known to have such a characteristic [ 15 , 53 , 54 ]. It is tempting to speculate that these small RNAs may possibly act as decoy RNA (small RNA sponge) to counteract antiviral RNA silencing, as previously demonstrated [ 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…Most of the members of the family Closteroviridae are pathogens of or associated with dicotyledonous crops, including vegetables and fruit trees. In recent studies, the de novo RNA-sequencing (RNA-seq) approach has enabled researchers to uncover many thus-far-unknown viral genome sequences in the family, including the members or candidates of the genus Closterovirus [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan

et al. 2023

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Many aphid-borne viruses are important pathogens that affect wheat crops worldwide. An aphid-transmitted closterovirus named wheat yellow leaf virus (WYLV) was found to have infected wheat plants in Japan in the 1970s; however, since then, its viral genome sequence and occurrence in the field have not been investigated. We observed yellowing leaves in the 2018/2019 winter wheat-growing season in an experimental field in Japan where WYLV was detected five decades ago. A virome analysis of those yellow leaf samples lead to the discovery of a closterovirus together with a luteovirus (barley yellow dwarf virus PAV variant IIIa). The complete genomic sequence of this closterovirus, named wheat closterovirus 1 isolate WL19a (WhCV1-WL19a), consisted of 15,452 nucleotides harboring nine open reading frames. Additionally, we identified another WhCV1 isolate, WL20, in a wheat sample from the winter wheat-growing season of 2019/2020. A transmission test indicated that WhCV1-WL20 was able to form typical filamentous particles and transmissible by oat bird-cherry aphid (Rhopalosiphum pad). Sequence and phylogenetic analyses showed that WhCV1 was distantly related to members of the genus Closterovirus (family Closteroviridae), suggesting that the virus represents a novel species in the genus. Furthermore, the characterization of WhCV1-WL19a-derived small RNAs using high-throughput sequencing revealed highly abundant 22-nt-class small RNAs potentially derived from the 3′-terminal end of the WhCV1 negative-strand genomic RNA, indicating that this terminal end of the WhCV1 genome is likely particularly targeted for the synthesis of viral small RNAs in wheat plants. Our results provide further knowledge on closterovirus diversity and pathogenicity and suggest that the impact of WhCV1 on wheat production warrants further investigations.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan

et al. 2023

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“…Alcea rosea L. (family: Malvaceae), also known as common hollyhock, is an annual, sometimes biennial plant native to China, but cultivars are cosmopolitan ornamentals in the tropics, subtropics and temperate climates. A number of viruses are known to affect A. rosea and include: hollyhock yellow vein mosaic virus (Srivastava et al 2014), hollyhock leaf crumple virus (Abdel-Salam et al 1998), tomato leaf curl virus (Ashwathappappa et al 2020) (Geminiviridae); zucchini yellow mosaic virus (Choi et al 2002), malva vein clearing virus (Menzel et al 2010) (Potyviridae) and alcea rosea virus 1 (Closteroviridae) (Wang et al 2020). Although the viruses known to infect A. rosea are diverse, observed symptoms have been mostly restricted to chlorotic mosaic and/or vein clearing of the leaves.…”

Section: Introductionmentioning

confidence: 99%