A new class of biological materials: Cell membrane-derived hydrogel scaffolds

Fan, Zhiyuan; Deng, Junjie; Li, Peter Y.; Chery, Daphney R.; Su, Yunfei; Zhu, Pu; Kambayashi, Taku; Blankenhorn, Elizabeth P.; Han, Lin; Cheng, Hao

doi:10.1016/j.biomaterials.2019.01.020

Cited by 56 publications

(37 citation statements)

References 69 publications

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“…Hydrogels with good flexibility and water‐storage performance belong to the class of highly promising functional materials. Their high hydrophilicity, water retention, and good biocompatibility endows them with extensive application prospects in biological tissue scaffold, controlled release of drug, adsorption and separation of pollutants, sensors, intelligent soft robots, petroleum engineering, mining engineering, and other fields. However, the traditional hydrogels are brittle.…”

Section: Introductionmentioning

confidence: 99%

Poly(acrylic acid)‐chitosan @ tannic acid double‐network self‐healing hydrogel based on ionic coordination

Zhang

et al. 2020

Polymers for Advanced Techs

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In this work, poly(acrylic) acid-chitosan @ tannic acid-aluminum ion (PAA-CS@TA-Al 3+ ) double-network hydrogel was prepared via prefabrication, blending method, and Al 3+ immersion method. The interaction between chitosan and tannic acid (CS@TA) was analyzed using Fourier transfer infrared spectra and UV-Vis spectra. The UV-Vis spectrum was also used to confirm the formation of ionic coordination in the gel. Then, the possible coordination modes were studied and analyzed. The microscopic pore structure and macroscopic strain behavior of the gel were analyzed using SEM and tensile testing, respectively, which verified that the tensile strength (≈32 KPa) and elongation at break (≈1700%) of the gel primarily resulted from its crosslinking structure. In addition, the gel also demonstrated a good self-healing performance with recovery ≈92.2% at 60 minutes.Hence, the proposed novel self-healing gel can provide inspiration for the preparation of future self-healing gels. K E Y W O R D Schitosan, double-network hydrogel, ionic coordination, self-healing, tannic acid

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Section: Introductionmentioning

confidence: 99%

Poly(acrylic acid)‐chitosan @ tannic acid double‐network self‐healing hydrogel based on ionic coordination

Zhang

et al. 2020

Polymers for Advanced Techs

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“…In recent examples in this context, cell membrane vesicles are used as cross‐linking agents for hydrogels. [ 52 ] In addition, RBC membranes as coating for glucose sensors were explored. [ 53 ]…”

Section: Discussionmentioning

confidence: 99%

“…In recent examples in this context, cell membrane vesicles are used as cross-linking agents for hydrogels. [52] In addition, RBC membranes as coating for glucose sensors were explored. [53] Over the last years, the use of cell membranes as coating has advanced toward applications in sensing, potential treatments of a variety of medical conditions, the use of increasing diverse types of cell sources, and the increased consideration of hybrid cell membranes on the way to become a clinically relevant concept.…”

Section: (10 Of 11)mentioning

confidence: 99%

Cell Membrane Coated Particles

Spanjers

Städler

2020

Adv. Biosys.

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that red blood cells (RBCs) circulate over 100 days although they are larger than the smallest capillaries. Nature not only employs the surface chemistry of the cells, but also their geometry and mechanical properties, i.e., the biconcave shape of the RBCs, give them the deformability to pass capillaries. Therefore, efforts to mimic RBCs are being reported from fabricating geometrically matching entities to matching their chemical composition. [2] However, making an artificial copy of a cell is challenging. Hence, the compromise between relying on a single interaction point (typically receptor-antibody interactions) of nanoformulations with cells and tissue, and the great complexity of an entire cell is currently a more realistic approach. An interesting example in this context is the use of purified cell membrane to camouflage (nano)particles. This concept offers opportunity to mimic cell−cell interactions employing multiple types of contact points while avoiding the need to obtain/purify specific proteins. This nature-inspired approach was first shown in 2011 with RBC membranes to enhance particle blood circulation times and developed in the next 4-5 years to include white blood cells, platelets, and cancerous cell sources. [3] The field gained increasing attention over the past years, expanding on types of cell sources, coated particles, and envisioned applications. This progress report will highlight selected efforts in the field from the past 2-3 years focusing on mammalian cell sources and coating of colloidal systems (Scheme 1). Efforts pre-dating 2017/2018 were already discussed in detail in several reviews. [4] First, a short overview over the general cell membrane purification procedure and subsequent characterization will be provided. Then, we will outline advances made when non-nuclei or nuclei containing cells sources were used. Finally, coatings consisting of two types of cell membrane (hybrid coatings) will be discussed 2. Formation and Characterization of Cell Membrane Coated Particles Despite of the variation in cell sources, the general purification methods of cell membranes and sequential coating of particles are rather similar and therefore described only briefly. Furthermore, important characterization techniques for size, protein content, and surface charge are discussed. We would like to note that there are a variety of other techniques not mentioned here specifically that can provide valuable specific information such as Fourier transform infrared spectroscopy for analysis of Nanoformulations are widely considered in the biomedical field for drug delivery, imaging, or detoxification purposes. Cell membrane coatings are a growing concept that aims to camouflage nanomaterials. The cell membranes are the first point of contact for cells to other biological or synthetic materials and nature has established signaling pathways in this context. In contrast to using purified membrane associated proteins, the use of purified cell membranes contains the protein of interest in a very native environme...

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“…[1,2] Naturally, molecules pass across biological membranes by either active transport [3][4][5][6] or passive diffusion. [19][20][21][22][23][24][25][26][27][28] However, artificial membrane channels created so far mainly rely on complicated chemical synthesis and are inefficient to dynamically control the transport process. [19][20][21][22][23][24][25][26][27][28] However, artificial membrane channels created so far mainly rely on complicated chemical synthesis and are inefficient to dynamically control the transport process.…”

Section: Doi: 101002/marc201900518mentioning

confidence: 99%

Mechanical Deformation Mediated Transmembrane Transport

Ming

Pang

Liu

2019

Macromol. Rapid Commun.

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Ideal methods to regulate transmembrane transport should be simple and able to accurately manipulate transmembrane transport without restructuring molecules.Here, we describe a novel approach in which we regulate transmembrane transport of molecules by mechanical deformation (Figure 1a), meeting these requirements, supported by its capability to precisely control the transmembrane diffusion of molecules. By mechanically deforming liposomal bilayers covalently embedded in a crosslinked hydrogel network (Figure 1b), we can loosen the compactness of phospholipid arrangement to trigger the diffusional transport of molecules. With the assistance of this new strategy, we are able to tune the transport across the lipid bilayer by simply stretching and loosening. Transmembrane diffusion of molecules can be initiated and ceased, and accurately adjusted by varying strain. Besides applying external mechanical force, the entire transport process can be well regulated without restructuring molecules.We prepared liposomal bilayers loaded hydrogels (LLHs) by a one-step aqueous radical polymerization of acrylamide using both polyethylene glycol dimethacrylate and acrylamide-decorated liposomes as crosslinkers. Fluorescent probes including 5,6-carboxyfluorescein (CF) and doxorubicin (Dox) were encapsulated inside the liposomes to facilitate observation and measurements. Transmission electron microscopy and dynamic light scattering results showed that the liposomes had a uniform size with an average diameter of 208 ± 10 nm ( Figure S1a,b, Supporting Information). It is worth noting that no influences were observed on the stability of liposomes after mixing with hydrogel precursors (Figure S1c,d, Supporting Information). The successful crosslinking of liposomes was confirmed by forming a solid gel in the absence of polyethylene glycol dimethacrylate (Figure 2a; Figure S2, Supporting Information). As expected, the gels dissolved quickly after treating with Triton X-100, a reagent that can disrupt phospholipid bilayers [31] and decrosslink the polymer network. Oscillatory rheological measurements indicate that LLHs exhibited higher storage moduli than gels prepared by using unmodified liposomes (without surface acrylamide groups), as displayed in Figure S3, Supporting Information. The resultant LLHs were highly stretchable (Figure 2b). The tensile strength and fracture strain were 27 ± 3 kPa and 6.3 ± 1, respectively, as recorded by tensile testing. Scanning electron microscopy (SEM) images Transmembrane transport is essential and plays critical roles for molecule exchange for cell survival. Methods capable of mimicking and regulating transmembrane transport have transformed the ability to create biosensors, separation membranes, and drug carriers. However, artificial channels have been largely restricted by their complicated chemical fabrication and inefficiency to dynamically manipulate the transport process. Here, a novel approach to regulate transmembrane transport is described by simply adjusting the mechanical deformation of li...

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A new class of biological materials: Cell membrane-derived hydrogel scaffolds

Cited by 56 publications

References 69 publications

Poly(acrylic acid)‐chitosan @ tannic acid double‐network self‐healing hydrogel based on ionic coordination

Poly(acrylic acid)‐chitosan @ tannic acid double‐network self‐healing hydrogel based on ionic coordination

Cell Membrane Coated Particles

Mechanical Deformation Mediated Transmembrane Transport

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