A New Centric Fusion Translocation in Cattle, Rob(16;18)

Abstract

A Barrosã bull (Portugal) has been found to carry a new Robertsonian translocation involving chromosomes 16 and 18 of standard cattle karyotype, as demonstrated by GBG- and RBG-banding techniques. C-banding patterns revealed the dicentric nature of this translocation. A comparison between normal cattle chromosome 16 and the q-arms of translocation chromosome and river buffalo chromosome 5 revealed the same G- and R-banding patterns, with only exception of a pericentromeric G-positive band which has been lost in river buffalo 5q and conserved in normal cattle chromosome 16 and rob(16;18) q-arms.

The Cytogenetics of the Water Buffalo: A Review

“…These chromosome changes have been the result of karyotype evolution, determining several species. Furthermore, the study of chromosomes has been mainly used in animal cytogenetics to (1) verify the relationship between chromosome abnormalities and fertility [ 3 , 4 , 5 , 6 , 7 ]; (2) physically map both type I (expressed sequences) and type II (SSRs, microsatellite marker, STSs) loci, especially using fluorescence in situ hybridization (FISH) techniques [ 8 , 9 , 10 , 11 , 12 ]; (3) correctly identify the chromosomes involved in chromosomal abnormalities via chromosome banding techniques [ 13 , 14 ]; (4) reveal chromosome rearrangements occurring in some chromosomal abnormalities, especially using both FISH mapping [ 15 , 16 , 17 ] and comparative genome hybridization array (aCGH) techniques [ 18 , 19 ]; (5) compare related and unrelated genomes by using the Zoo-FISH technique [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ], centromeric SAT sequences by FISH mapping [ 29 ], or detailed FISH mapping along chromosomes [ 30 , 31 , 32 , 33 , 34 , 35 , 36 ]; and (6) test the genome stability of several bovids, including the river buffalo, with both in vitro and in vivo (natural) exposure to potential mutagens [ 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ], or affected by limb malformation...…”

Robertsonian translocation as a result of telomere shortening during replicative senescence and immortalization of bovine oviduct epithelial cells

“…Chromosomes 1, 4, 13, 16, and 21 have multiple partners (Miyake et al 1991;Iannuzzi et al 1993;Hanada et al 1995;Geshi et al 1996;Pearce et al 1997;Molteni et al 1998;Rubes et al 1999). These combined results suggest that particular chromosomes might preferentially fuse with other chromosomes in cattle cells and stably remain as fused chromosomes in somatic cells in vivo.…”

A New Robertsonian Translocation in Cattle, Rob(16;20)

“…5). Dicentric fusion is common to many other reported translocations: (LOCUE and HARVEY 1978;CRIBIU et al 1989;IANNUZZI et al 1993;BAHRT-DARWICH et al 1993;BERLAND et al 1988;ELLSWORTH et al 1979;SLOTA and SWITONSKI 1992;DI BERARDINO et al 1979;MIYAKE et al 1991). There is general agreement that dicentric fusions are unstable and can disappear after only a few cell divisions: therefore, more observed dicentric fusions are probably of recent origin (IANNUZZI et al 1992;POPESCU 1977).…”

“…The bull carrying the new translocation rob(16;20) was phenotypically normal, as is the case for other cattle with various centric fusions (IANNUZZI et al 1993;SLOTA and SWITONSKI 1992;BAHRI-DARWICH et al 1993;MIYAKE et al 1991;POLLOCK and BOWMAN 1974). Only a few cases of altered phenotype have been described in cattle with Robertsonian translocations, and these phenotypic alterations appear to be coincidental (ELLSWORTH et al 1979; 1987,1988).…”

“…Both chromosomes 16 and 20 have been shown to be involved in other centric fusion translocations. Specifically, chromosome 16 in rob( 16;18) (IANNUZZI et al 1993) and chromosome 20 in rob( 14;20) ( LOGUE and HARVEY 1978). The 14/20 centric fusion aberration appears to be unique to the Simmental breed.…”