A new cAMP response element in the transcribed region of the human c-fos gene

Härtig, E.; Lončarević, Ivan F.; Büscher, Marita; Herrlich, Peter; Rahmsdorf, Hans J.

doi:10.1093/nar/19.15.4153

Cited by 40 publications

(22 citation statements)

References 51 publications

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“…CRE is one of the important cis DNA elements in the c-fos gene promoter. 23 Infection of AdCREBM1 strongly inhibited thrombin-induced c-fos mRNA expression in VSMCs ( Figure 4B). …”

Section: Inhibition Of Thrombin-induced C-fos Mrna Expression By Overmentioning

confidence: 90%

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cAMP Response Element-Binding Protein Mediates Thrombin-Induced Proliferation of Vascular Smooth Muscle Cells

Tokunou

Ichiki

Takeda

et al. 2001

ATVB

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Abstract-Thrombin is a potent mitogen for vascular smooth muscle cells (VSMCs) and plays an important role in the progression of atherosclerosis. Although recent reports have suggested that cAMP response element-binding protein (CREB) is necessary for the survival of neuronal cells, the role of CREB in VSMC proliferation is not determined. We examined the role of CREB in thrombin-induced VSMC proliferation and the effect of thrombin on phosphorylation of CREB at Ser133, which is a critical marker for activation by Western blot analysis. Thrombin induced phosphorylation of CREB in a dose-dependent manner. An oligopeptide, SFLLRN, which activates the thrombin receptor, also induced the phosphorylation of CREB. Inhibition of extracellular signal-regulated protein kinase or inhibition of p38 mitogen-activated protein kinase suppressed the thrombin-induced CREB phosphorylation. Inhibition of the epidermal growth factor receptor by AG1478 also inhibited the thrombin-induced CREB phosphorylation. Overexpression of the dominant-negative form of CREB inhibited thrombin-induced c-fos mRNA expression and incorporation of

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“…CRE is one of the important cis DNA elements in the c-fos gene promoter. 23 Infection of AdCREBM1 strongly inhibited thrombin-induced c-fos mRNA expression in VSMCs ( Figure 4B). …”

Section: Inhibition Of Thrombin-induced C-fos Mrna Expression By Overmentioning

confidence: 90%

“…23 CRE in the promoter region plays a critical role in the induction of c-fos gene expression in response to mitogens. We showed that overexpression of the dominant-negative form of CREB strongly suppressed thrombin-induced c-fos mRNA expression, confirming the previous results.…”

Section: Discussionmentioning

confidence: 99%

cAMP Response Element-Binding Protein Mediates Thrombin-Induced Proliferation of Vascular Smooth Muscle Cells

Tokunou

Ichiki

Takeda

et al. 2001

ATVB

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“…However, there are several key differences in the promoters of the two genes as well. The c-fos promoter contains two cyclic adenosine monophosphate (cAMP) binding protein response (CRE)-like elements upstream of the transcription start site (TATA box) that confers enhancement of c-fos expression via cAMP (Hartig et al, 1991). The fra-2 promoter lacks a TATA box and has only one CRE (Yoshida et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Restraint-induced fra-2 and c-fos expression in the rat forebrain: Relationship to stress duration

2007

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The protein product of the fra-2 gene (Fra-2), a fos-family member, can compete with Fos protein for participation in AP-1 transcription factor complexes and each protein can contribute different transactivational consequences to an AP-1 complex. To date, there is limited characterization of fra-2 mRNA expression in the rat forebrain. We examined basal and restraint-induced mRNA expression (in situ hybridization) of fra-2 in the rat forebrain and compared its temporal-spatial pattern to c-fos. In contrast to the very low basal expression of c-fos, fra-2 basal expression was moderately high throughout cortex and some subcortical structures, including prominent basal expression in the hypothalamic paraventricular nucleus (PVN). Restraint-induced fra-2 expression was quantified in the prefrontal cortex (PFC), lateral septum (LS) and PVN. Maximal fra-2 gene induction in the PFC and LS was delayed (60 min) after restraint onset with respect to c-fos (15 min), whereas in the PVN, fra-2 mRNA increased within 15 min of restraint. Additionally we compared c-fos and fra-2 gene expression in rats given shorter or longer restraint durations, but equal total time from stress onset to sample collection, to determine the extent to which the kinetics of gene induction matched that of a hypothalamic-pituitary-adrenal axis hormone response. Rats given 45 min recovery after 15 min restraint showed less c-fos expression in the PVN, less fra-2 expression in the prelimbic and infralimbic PFC, and no difference in the LS compared with rats restrained for 60 min. Thus, the expression of both genes was sensitive to stressor duration, but this sensitivity varied with brain region. Differential basal and stress-induced expression patterns of the fra-2 and c-fos genes are likely to have important functional consequences for AP-1 transcription factor dependent regulation of neural plasticity.

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“…For example, bovine CYPJ7 gene (38) and human c-fos gene (39) have been shown to be tightly regulated by cAMP but consensus CRE sequences have not been found in the 5' regulatory regions of these genes. It is of interest that Godfrey et al (25) reported the presence of an AP-2-like sequence (TGGGGA) in the 5' regulatory region of a MNP gene of P. chrysosporium.…”

Section: Discussionmentioning

confidence: 99%

cAMP-mediated differential regulation of lignin peroxidase and manganese-dependent peroxidase production in the white-rot basidiomycete Phanerochaete chrysosporium.

Boominathan

Reddy

1992

Proc. Natl. Acad. Sci. U.S.A.

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Lignin peroxidases (LIPs) and manganesedependent peroxidases (MNPs) are major components of the -ignin-degrading enzyme system of Phanerochaete chrysosporium and typically appear during secondary metabolism. The involvement of cAMP in the regulation of production of LIPs and MNPs was investigated in this study. Production of LIPs and MNPs was preceded by a sharp rise in intracellular cAMP concentration. Addition of atropine, theophylline, or histamine to cultures resulted in a drop in intracellular cAMP concentration and a concomitant inhibition of production of LIPs only or of both LIPs and MNPs, depending on the concentration of the inhibitor added. These results were independently confirmed by fast protein liquid chromatographic proffles of the LIPs and MNPs in the extracellular fluid of the inhibitor-treated and untreated control cultures. LIP production was generally more sensitive to the inhibitors than MNP production. Northern blot analyses showed that the inhibitors affect the production of LIPs and MNPs at the level of transcription. Furthermore, LIP and MNP gene expression appears to be differentially regulated depending on the intracellular concentration of cAMP. These results show that cAMP plays a key role in the regulation of production of LIPs and MNPs in P. chrysosporium.The white-rot basidiomycete Phanerochaete chrysosporium has been the focus of intense worldwide research from the standpoint of lignin biodegradation and bioremediation of environmental pollutants (for review, see refs.

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A new cAMP response element in the transcribed region of the human c-fos gene

Cited by 40 publications

References 51 publications

cAMP Response Element-Binding Protein Mediates Thrombin-Induced Proliferation of Vascular Smooth Muscle Cells

cAMP Response Element-Binding Protein Mediates Thrombin-Induced Proliferation of Vascular Smooth Muscle Cells

Restraint-induced fra-2 and c-fos expression in the rat forebrain: Relationship to stress duration

cAMP-mediated differential regulation of lignin peroxidase and manganese-dependent peroxidase production in the white-rot basidiomycete Phanerochaete chrysosporium.

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