A new broad specificity alkaline metalloprotease from a Pseudomonas sp. isolated from refrigerated milk: Role of calcium in improving enzyme productivity

Ertan, Haluk; Cassel, Camilo; Verma, Arjun; Poljak, Anne; Charlton, Tim; Aldrich‐Wright, Janice R.; Omar, Suhaila Mohd; Siddiqui, Khawar Sohail; Cavicchioli, Ricardo

doi:10.1016/j.molcatb.2014.12.010

Cited by 19 publications

(30 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The turns between strands are composed of a repeated GGxGxD motif. In these repeats, Ca 2+ is coordinated to the carboxylate groups of the aspartic acid side chains and the carbonyl groups of the glycine backbones, thereby being stabilized by these six‐residues‐long repeats (Ertan et al., ). This motif is characteristic of proteins secreted via the type I secretion process (Chabeaud et al., ; Matéos et al., ). A high content of hydrophobic amino acids. No cysteine residues and a high content of glycine residues (Marchand et al., ; Matéos et al., ).…”

Section: Biological Properties Of Protease Aprxmentioning

confidence: 99%

“…Two minerals have been found to be relevant for AprX production, calcium and iron. Calcium/Ca 2+ has been implicated as the most important inorganic compound in modulating the protease production from pseudomonads, because Ca 2+ is necessary for the protein structure of AprX (Ertan et al., ). Calcium‐stimulated protease production has also been reported for many Pseudomonas strains (Ertan et al., ; Nicodème et al., ).…”

Section: Biological Properties Of Protease Aprxmentioning

confidence: 99%

“…The turns Figure 2-X-ray structure of extracellular alkaline metalloprotease from Pseudomonas aeruginosa (1KAP). Zn 2+ ions in the N-terminal catalytic domain are shown in yellow; Zn 2+ binding histidine residues are in blue; catalytic glutamic acid residue is in red; putative methionine residues in the Met-turn characteristic of metzincins are in magenta, with Ca 2+ ions in the C-terminal domain shown in green, reproduced with permission from Ertan et al (2015).…”

Section: Biological Properties Of Protease Aprxmentioning

confidence: 99%

See 2 more Smart Citations

The Extracellular Protease AprX from Pseudomonas and its Spoilage Potential for UHT Milk: A Review

Zhang

Bijl

Svensson

et al. 2019

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

The negative effects of proteases produced by psychrotrophic bacteria on dairy products, especially ultra‐high‐temperature (UHT) milk, are drawing increasing attention worldwide. These proteases are especially problematic, because it is difficult to control psychrotrophic bacteria during cold storage and to inactivate their heat‐resistant proteases during dairy processing. The predominant psychrotrophic species with spoilage potential in raw milk, Pseudomonas, can produce a thermostable extracellular protease, AprX. A comprehensive understanding of AprX on the aspects of its biological properties, regulation, proteolytic potential, and its impact on UHT milk can contribute to finding effective approaches to minimize, detect, and inactivate AprX. AprX also deserves attention as a representative of all extracellular metalloproteases produced by psychrotrophic bacteria in milk. The progress of current research on AprX is summarized in this review, including a view on the gap in current understanding of this enzyme. Reducing the production and activity of AprX has considerable potential for alleviating the problems that arise from the instability of UHT milk during shelf‐life.

show abstract

Section: Biological Properties Of Protease Aprxmentioning

confidence: 99%

Section: Biological Properties Of Protease Aprxmentioning

confidence: 99%

Section: Biological Properties Of Protease Aprxmentioning

confidence: 99%

See 1 more Smart Citation

The Extracellular Protease AprX from Pseudomonas and its Spoilage Potential for UHT Milk: A Review

Zhang

Bijl

Svensson

et al. 2019

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

show abstract

“…It not only participates in neuromuscular transmission and blood clotting but also maintains the pH values of blood and electrolyte. Furthermore, it could adjust enzyme productivity . Especially, lack of calcium has been singled out as a major public health concern since it is critically important to bone health .…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, it could adjust enzyme productivity. [15][16][17] Especially, lack of calcium has been singled out as a major public health concern since it is critically important to bone health. [18][19][20] Quite a few diseases such as dysplasia, rickets, and osteoporosis can be attributed to the lack of calcium.…”

mentioning

confidence: 99%

Degradation performance of polyglutamic acid and its application of calcium supplement

Zhan

Chi

et al. 2018

Polymers for Advanced Techs

View full text Add to dashboard Cite

Degradable biopolymers with functional groups play a crucial role in the biomedical field. In this case, the influences of temperature and pH values on the degradation performance of poly (γ‐glutamic acid) (γ‐PGA) were fully explored by gel permeation chromatography. Further, γ‐PGA‐Ca was prepared by using calcium chloride to react with the low molecular weight γ‐PGA and characterized by Fourier transform infrared, differential scanning calorimetry test, gel permeation chromatography, atomic absorption spectrophotometric, Ca2+ release in vivo, and cytotoxicity experiments. Furthermore, Caco‐2 cell model was constructed to study the mechanism of γ‐PGA‐Ca intestinal absorption. Results indicated that low pH value and high temperature are the suitable conditions for the degradation of γ‐PGA. It also suggested that γ‐PGA‐Ca can be used as calcium supplements due to its high rate of absorption.

show abstract

Screening, characterization, and kinetic studies of a serine alkaline protease from kitchen wastewater bacteria P2S1An and evaluation of its application in nutraceutical production

Das

Ghosh

2023

Water Environment Research

View full text Add to dashboard Cite

This present investigation aimed at characterizing the biochemical potential and kinetic study of the protease isolated from kitchen wastewater bacteria, P2S1An. The enzymatic activity was optimum when incubated for 96 h, at 30°C and pH 9.0. The enzymatic activity of the purified protease (PrA) was 10.47‐folds that of crude protease (S1). PrA was about 35 kDa in molecular weight. The broad pH and thermal stability, chelators, surfactants and solvent tolerance, and favorable thermodynamics suggested the potentiality of the extracted protease PrA. Thermal activity and stability were enhanced in presence of 1‐mM Ca2+ ion at high temperatures. The protease was a serine one as its activity was completely diminished in presence of 1‐mM PMSF. The Vmax, Km, and Kcat/Km suggested stability and catalytic efficiency of the protease. PrA hydrolyzes fish protein with 26.61 ± 0.16% of peptide bond cleavage after 240 min, comparable to Alcalase 2.4L (27.13 ± 0.31%). Practitioner points A serine alkaline protease PrA was extracted from kitchen wastewater bacteria Bacillus tropicus Y14. Protease PrA showed significant activity and stability in a wide temperature and pH range. Protease showed great stability towards additives like metal ions, solvents, surfactants, polyols, and inhibitors. Kinetic study showed that the protease PrA had a prominent affinity and catalytic efficiency for the substrates. PrA hydrolysed fish proteins into short bioactive peptides which signify its potential in the formation of functional food ingredients.

show abstract

A new broad specificity alkaline metalloprotease from a Pseudomonas sp. isolated from refrigerated milk: Role of calcium in improving enzyme productivity

Cited by 19 publications

References 52 publications

The Extracellular Protease AprX from Pseudomonas and its Spoilage Potential for UHT Milk: A Review

The Extracellular Protease AprX from Pseudomonas and its Spoilage Potential for UHT Milk: A Review

Degradation performance of polyglutamic acid and its application of calcium supplement

Screening, characterization, and kinetic studies of a serine alkaline protease from kitchen wastewater bacteria P2S1An and evaluation of its application in nutraceutical production

Contact Info

Product

Resources

About