A New Biotin Derivative−DOTA Conjugate as a Candidate for Pretargeted Diagnosis and Therapy of Tumors

Sabatino, Giuseppina; Chinol, Marco; Paganelli, Giovanni; Papi, Stefano; Chelli, Mario; Leone, Giuseppe; Papini, Anna Maria; Luca, and Angelo De; Ginanneschi, Mauro

doi:10.1021/jm030789z

Cited by 44 publications

(50 citation statements)

References 11 publications

(28 reference statements)

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“…One possible problem in in vivo circumstances is the biotinidase enzyme (especially in plasma), which is capable of cleaving biotin-conjugates [80] and thereby lowering the targeting efficacy of the system. The development of biotin derivatives that are resistant to biotinidase, however, reduces the severity of this problem [81]. baculoviruses are taken up by a variety of cells, it would be useful to restrict viral transduction to the target cells.…”

Section: The Potential Of Novel Avidin Fusion Proteins In Medical Tarmentioning

confidence: 99%

Avidin Fusion Protein Strategies in Targeted Drug and Gene Delivery

Laitinen¹,

Airenne²,

Räty³

et al. 2005

LDDD

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Chicken avidin is used in a wide variety of applications in the life sciences, due to its extraordinarily strong affinity for biotin. Avidin-biotin strategy has also recently established a role in medical targeting approaches for cancer treatments. An interesting new exploitation for avidin-biotin techniques is the utilization of this strong affinity in gene therapy, to target and enhance gene delivery to the appropriate target cells and tissues. In this communication, we review novel avidin fusion proteins that have been developed for enhanced gene delivery and drug targeting purposes. The principles of this approach are highlighted by the avidin fusion proteins Scavidin (fusion with the macrophage scavenger receptor class A), Lodavin (fusion with the endocytotic LDL receptor) and avidin-displaying baculovirus (Baavi). Scavidin and Lodavin are cell membrane proteins in which avidin is expressed as an extracellular portion, thereby enhancing the targeted drug and gene delivery with biotinylated drug molecules and vectors. Baavi, on the other hand, displays avidin moieties on its surface, and opens up possibilities for flexible modification of its targeting properties and other characteristics by using different biotin-conjugated molecules. Potential applications of avidin fusion proteins and future trends of targeted drug and gene delivery based on these strategies will be discussed.

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Section: The Potential Of Novel Avidin Fusion Proteins In Medical Tarmentioning

confidence: 99%

Avidin Fusion Protein Strategies in Targeted Drug and Gene Delivery

Laitinen¹,

Airenne²,

Räty³

et al. 2005

LDDD

View full text Add to dashboard Cite

show abstract

“…The monomer units combine into the active form, 2,3 so each avidin unit is capable of binding four biotin molecules. 3,4 The avidin-biotin system has been used as a tool for various purposes, such as the diagnosis 5 of, for example, tumours 6 and for isolation (affinity chromatography). 7 The intention of the present work was to use the avidin-biotin system in combination with positron emission tomography (PET) imaging to follow the fate of the transplanted islets of Langerhans.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of ¹⁸F‐labelled biotin analogues

Blom

Itsenko

Långström

2011

Labelled Comp Radiopharmac

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A one-step 18 F-labelling strategy was used to prepare three labelled analogues of the vitamin biotin, which can be useful as tracers because of biotin's high affinity for avidin. The labelled compounds were obtained in decay-corrected yields of up to 35% and specific radioactivity of 320GBq/mmol. When evaluated in situ, the analogues showed good affinity for avidin: 60-75% of the radiolabelled compounds were bound to avidin within 5minutes. The binding was site-specific, as shown by blocking experiments with native biotin.

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“…68 Ga-1,4,7,10-tetra-azacyclododecane-1,4,7,10-tetraacetic acidTyr3-octreotide (DOTA-TOC), 68 Ga-DOTA-1-Nal-octreotide (DOTA-NOC), 68 Ga-DOTA-bombesin, 68 Ga-1,4,7-triazacyclononanetriacetic acid (NOTA)-RGD, 68 Ga-DOTAalbumin, and 68 Ga-DOTA-human epidermal growth factor (hEGF) are examples of such agents (Fig. 1) [8,[10][11][12][13][14][15]. Similarly, some agents, such as 68 Ga-[(4,6-MeO 2 sal) 2-BAPEN] + and 68 Ga-N 2 S 2 , are chelates of radioactive gallium and used for myocardial imaging [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

68Ga-Labeled Radiopharmaceuticals for Positron Emission Tomography

Shetty

Lee

Jeong

2010

Nucl Med Mol Imaging

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68 Ga is a promising emerging radionuclide for positron emission tomography (PET). It is produced using a 68 Ge/ 68 Ga-generator, and thus, would enable the cyclotronindependent distribution of PET. However, new 68 Galabeled radiopharmaceuticals that can replace 18 F-labeled agents like [ 18 F]fluorodeoxyglucose (FDG) are needed. Most of the 68 Ga-labeled derivatives currently used are peptide agents, but the developments of other agents, such as amino acid derivatives, nitroimidazole derivatives, and glycosylated human serum albumin, are being actively pursued in many laboratories. Thus, appearance of new 68 Ga-labeled radiopharmaceuticals with high impact are expected in the near future. Here, we present an overview of 68 Ga-labeled agents in terms of their clinical significances and relevances to the management of certain tumors, and pertinent pre-clinical developments.

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A New Biotin Derivative−DOTA Conjugate as a Candidate for Pretargeted Diagnosis and Therapy of Tumors

Cited by 44 publications

References 11 publications

Avidin Fusion Protein Strategies in Targeted Drug and Gene Delivery

Avidin Fusion Protein Strategies in Targeted Drug and Gene Delivery

Synthesis of ¹⁸F‐labelled biotin analogues

68Ga-Labeled Radiopharmaceuticals for Positron Emission Tomography

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A New Biotin Derivative−DOTA Conjugate as a Candidate for Pretargeted Diagnosis and Therapy of Tumors

Cited by 44 publications

References 11 publications

Avidin Fusion Protein Strategies in Targeted Drug and Gene Delivery

Avidin Fusion Protein Strategies in Targeted Drug and Gene Delivery

Synthesis of 18F‐labelled biotin analogues

68Ga-Labeled Radiopharmaceuticals for Positron Emission Tomography

Contact Info

Product

Resources

About

Synthesis of ¹⁸F‐labelled biotin analogues