A new biomimetic route to engineer enzymatically active mechano-responsive materials

Rios, César; Longo, Johan; Zahouani, Sarah; Garnier, Tony; Vogt, Cédric; Reisch, Andreas; Senger, Bernard; Boulmedais, Fouzia; Hemmerlé, Joseph; Benmlih, Karim; Frisch, Benoı̂t; Schaaf, Pierre; Jierry, Loı̈c; Lavalle, Philippe

doi:10.1039/c5cc00329f

Cited by 18 publications

(20 citation statements)

References 22 publications

(22 reference statements)

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“…One of the most widely used L‐b‐L film formulation with HA is based on poly‐L‐Lysine (PLL, polycation) and HA (polyanion) which was first developed in our laboratory, as shown in Figure c–e. A large and continuous green fluorescent zone demonstrates that the PLL has diffused within the whole film.…”

Section: Use Of Ha and Its Derivatives In Coatings Polyelectrolyte Mmentioning

confidence: 99%

Hyaluronic Acid and Its Derivatives in Coating and Delivery Systems: Applications in Tissue Engineering, Regenerative Medicine and Immunomodulation

Knopf‐Marques

Pravda

Wolfová

et al. 2016

Adv Healthcare Materials

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180

159

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epithelial, connective, and nerve tissues of vertebrates. It is designated as a glycosaminoglycan. [1] Glycosaminoglycans are composed of disaccharide blocks of N-acetylgalactosamine or N-acetylglucosamine, (amino sugars) and uronic sugars such as glucuronic acid, iduronic acid or galactose. This group of heteropolysaccharides comprises HA, chondroitin sulfates, dermatan sulfate, heparin and heparin sulfates whose sulfation degree is less than that of heparin. Unlike other glycosaminoglycans, hyaluronan is not sulfated and it is self-standing, i.e. without an association with a core protein. [2] It was isolated in 1934 by Meyer and Palmer from bovine vitreous humor. [3] The polymer chain of hyaluronan comprises repeating disaccharide units where the pyranose rings are connected by β-1,3 bonds. The repeating units are bonded with a β-1,4 glycosidic bond within the chain between N-acetyl-D-glucosamine and D-glucuronic acid. At physiological pH each glucuronate unit, associated with its carboxylate group, carries an anionic charge. The hundreds of negative charges are fixed to each chain. These anionic groups are balanced with mobile cations such as Na + , K + , Ca 2+ and Mg 2+ . During ionization of D-glucuronic acid with the carboxylic groups, the charges influence the organization of the chains and their interactions with their surroundings. In turn, they are affected by pH and ionic strength. The chain organization and charge directly affect the solubility in water, since hyaluronan is water-insoluble when convertedAs an Extracellular Matrix (ECM) component, Hyaluronic acid (HA) plays a multi-faceted role in cell migration, proliferation and differentiation at micro level and system level events such as tissue water homeostasis. Among its biological functions, it is known to interact with cytokines and contribute to their retention in ECM microenvironment. In addition to its biological functions, it has advantageous physical properties which result in the industrial endeavors in the synthesis and extraction of HA for variety of applications ranging from medical to cosmetic. Recently, HA and its derivatives have been the focus of active research for applications in biomedical device coatings, drug delivery systems and in the form of scaffolds or cell-laden hydrogels for tissue engineering. A specific reason for the increase in use of HA based structures is their immunomodulatory and regeneration inducing capacities. In this context, this article reviews recent literature on modulation of the implantable biomaterial microenvironment by systems based on HA and its derivatives, particularly hydrogels and microscale coatings that are able to deliver cytokines in order to reduce the adverse immune reactions and promote tissue healing.

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Section: Use Of Ha and Its Derivatives In Coatings Polyelectrolyte Mmentioning

confidence: 99%

Hyaluronic Acid and Its Derivatives in Coating and Delivery Systems: Applications in Tissue Engineering, Regenerative Medicine and Immunomodulation

Knopf‐Marques

Pravda

Wolfová

et al. 2016

Adv Healthcare Materials

Self Cite

180

159

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“…The effects of mechanical deformation on the functional properties of proteins and other bioactive molecules immobilized on polymeric supports have been studied for nearly 40 years [133,134]. Immobilization of an enzyme molecule on a polymer support followed by mechanical deformation of this support can result in deformation of the protein globule and considerable change of enzyme activity [135]. Such approaches can be further extended to single molecule studies to modulate biocatalysis, membrane transport, protein synthesis and other essential biological functions by mechanically affecting enzymes, membrane channels, ribosomes, etc.…”

Section: Observation Of Magneto-mechanical Actuation In Biologicalmentioning

confidence: 99%

Towards nanomedicines of the future: Remote magneto-mechanical actuation of nanomedicines by alternating magnetic fields

Golovin

Gribanovsky

Golovin

et al. 2015

Journal of Controlled Release

190

156

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The paper describes the concept of magneto-mechanical actuation of single-domain magnetic nanoparticles (MNPs) in super-low and low frequency alternating magnetic fields (AMFs) and its possible use for remote control of nanomedicines and drug delivery systems. The applications of this approach for remote actuation of drug release as well as effects on biomacromolecules, biomembranes, subcellular structures and cells are discussed in comparison to conventional strategies employing magnetic hyperthermia in a radio frequency (RF) AMF. Several quantitative models describing interaction of functionalized MNPs with single macromolecules, lipid membranes, and proteins (e.g. cell membrane receptors, ion channels) are presented. The optimal characteristics of the MNPs and an AMF for effective magneto-mechanical actuation of single molecule responses in biological and bio-inspired systems are discussed. Altogether, the described studies and phenomena offer opportunities for the development of novel therapeutics both alone and in combination with magnetic hyperthermia.

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“…The homemade stretching device used for the experiments was composed of two jaws made of stainless steel allowing to tighten the films and to stretch them manually in a uniaxial direction. 5 The stretching rate (or strain) ε has been defined by the relation ε = 100 × (L − L 0 )/L 0 (in %) where L 0 and L represent respectively the lengths in the nonstretched and in the stretched states. The experiments were performed at room temperature in the presence of buffer solution (NaCl 0.15 M/Tris 10 mM, pH 7.4) to avoid the drying of the films.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…Moreover, one can expect that the processes onto which soft-mechanochem-istry is based are at least partially reversible. There already exist several examples of soft-mechanochemistry processes such as the stretch-induced modulation of enzymatic reactions 4,5 or the reversible tuning of the torsion angle of amphiphilic chiral molecules at the air/water interface by application of a mechanical force at a molecular monolayer. 8 According to several theoretical predictions, a coil-to-helix transition can be induced by stretching macromolecules which are close to the spontaneous coil-to-helix transition.…”

Section: ■ Introductionmentioning

confidence: 99%

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Stretch-Induced Helical Conformations in Poly(l-lysine)/Hyaluronic Acid Multilayers

Zahouani

Chaumont

Senger

et al. 2015

ACS Appl. Mater. Interfaces

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We investigate the effect of stretching on the secondary structure of cross-linked poly(l-lysine)/hyaluronic acid (PLL/HA) multilayers. We show that stretching these films induces changes in the secondary structure of PLL chains. Our results suggest that not only α- but also 310-helices might form in the film under stretching. Such 310-helices have never been observed for PLL so far. These changes of the secondary structure of PLL are reversible, i.e., when returning to the nonstretched state one recovers the initial film structure. Using molecular dynamics simulations of chains composed of 20 l-lysine residues (PLL20), we find that these chains never adopt a helical conformation in water. In contrast, when the end-to-end distance of the chains is restrained to values smaller than the mean end-to-end distance of free chains, a distance domain rarely explored by the free chains, helical conformations become accessible. Moreover, the formation of not only α- but also 310-helices is predicted by the simulations. These results suggest that the change of the end-to-end distance of PLL chains in the stretched film is at the origin of the helix formation.

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A new biomimetic route to engineer enzymatically active mechano-responsive materials

Cited by 18 publications

References 22 publications

Hyaluronic Acid and Its Derivatives in Coating and Delivery Systems: Applications in Tissue Engineering, Regenerative Medicine and Immunomodulation

Hyaluronic Acid and Its Derivatives in Coating and Delivery Systems: Applications in Tissue Engineering, Regenerative Medicine and Immunomodulation

Towards nanomedicines of the future: Remote magneto-mechanical actuation of nanomedicines by alternating magnetic fields

Stretch-Induced Helical Conformations in Poly(l-lysine)/Hyaluronic Acid Multilayers

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