A new biological approach in generating an irritable bowel syndrome rat model – focusing on depression in sucrose splash test and body weight change

Lefter, Radu; Ciobîcă, Alin; Timofte, Daniel; Ababei, Daniela Carmen; Dobrin, Romeo; Luca, Andrei; Trifan, Anca; Stanciu, C; Sfarti, Cătălin

doi:10.25083/rbl/25.3/1554.1562

Cited by 8 publications

(8 citation statements)

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“…In order to induce the IBS symptoms to rats, we used a combined multifactorial heterotypic chronic and subchronic stress-exposure paradigm, as described with some variations by previous studies in the literature [48, 49]. For the current experiment, we adapted the method by using an original combination of stressors in order to optimize the protocol and reduce the time of exposure, which we presented previously [50]: thus, the IBS groups were exposed each day to two stress factors for a relative short period of only seven days. First stressor was constantly used each morning and succeeded by a second stress factor that differed daily in order to minimize habituation.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Capacity and Behavioral Relevance of a Polyphenolic Extract of Chrysanthellum americanum in a Rat Model of Irritable Bowel Syndrome

Cojocariu

Ciobîcă

Balmuș

et al. 2019

Oxidative Medicine and Cellular Longevity

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Chrysanthellum americanum L. (Vatke) is a medicinal plant from the Compositae family used in west-African traditional medicine, known for its flavonoid and saponin richness and for its strong antioxidant potential. In the present study, we assessed the effects of Chrysanthellum americanum polyphenolic extract in the psychological stress-induced rat model of irritable bowel syndrome (IBS), a chronic functional digestive tract disorder marked by immune and inflammatory-related disturbances of central nervous and peripheral intestinal systems, which is often associated with mood disorders including depression and anxiety. Consequently, memory impairment, anxiety and depression behavioral indicators, and cerebral oxidative stress biomarker dynamics were evaluated in a multifactorial heterotypic stress-exposed IBS rats after 6-day gavage with polyphenolic C. americanum extract (100 mg/kg body weight). Y-maze, elevated plus maze, and forced swimming tests were used for assessing behavioral responses. Administration of the extract exhibited significant anxiolytic and antidepressant-like effects coupled with significantly increased temporal lobe antioxidant enzyme specific activity (superoxide dismutase and glutathione peroxidase) and decreased malondialdehyde levels, a well-known lipid peroxidation marker. Furthermore, linear regression statistical analyses showed significant correlations between the oxidative stress parameters and behavioral tests. In conclusion, our results suggest that the administration of Chrysanthellum americanum polyphenolic extract could ameliorate mood and cognitive disturbances related to stress-induced in an IBS rat model. This could be also related to cerebral oxidative stress status attenuation.

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Section: Methodsmentioning

confidence: 99%

Antioxidant Capacity and Behavioral Relevance of a Polyphenolic Extract of Chrysanthellum americanum in a Rat Model of Irritable Bowel Syndrome

Cojocariu

Ciobîcă

Balmuș

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Depression-like behavior was assessed using the sucrose splash test. The rat grooming behavior following spraying the sucrose solution on the dorsal coat reflects self-care and motivational behavior ( Lefter et al, 2018 ). The sucrose splash test was performed in the open-field apparatus.…”

Section: Methodsmentioning

confidence: 99%

“…Each rat was splashed two times with 10% sucrose solution on the dorsal coat using an atomizer spray bottle. After applying the sucrose solution, the total time spent in grooming, the latency to the first bout of grooming, and the number of grooms within a 5 min test period were recorded as indices of depression-like behavior ( Ortmann et al, 2016 ; Hu et al, 2017 ; Lefter et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Early-Life Sleep Deprivation Enhanced Alcohol Consumption in Adolescent Rats

et al. 2022

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Evidence in the literature suggests that sleep deprivation during early-life developmental stages, by impacting important processes such as the reward circuit maturation, may increase the vulnerability for alcohol and substance use. The mechanisms involved are not fully understood. In this study, utilizing our previously established model, we examined the impact of early-life sleep deprivation on alcohol consumption in adolescent rats. Male Sprague Dawley rats served as either the control (CON) or sleep-deprived (SD) group. Sleep deprivation was induced using a Pinnacle automated sleep deprivation apparatus. The SD group of rats was sleep deprived for 6–8 h/day for 14 days from postnatal day (PND)19 to PND32. At PND33, anxiety- and depression-like behaviors were assessed in rats using elevated plus maze and sucrose splash test, respectively. At PND39, alcohol consumption was assessed in rats for five consecutive days using the two-bottle choice paradigm, water versus 5% ethanol. SD rats exhibited significant anxiety- and depression-like behaviors as compared to CON rats. Interestingly, SD rats consumed a larger volume of alcohol when compared to CON rats, which was significantly higher at day 5 (mean of alcohol consumption (ml) ± SD; CON = 6.67 ± 3.42; SD = 19.00 ± 6.05, p = 0.0126). SD rats also showed high preference for alcohol over water, which was significantly higher at day 5 (mean of alcohol preference (%) ± SD; CON = 26.85 ± 14.97; SD = 57.69 ± 5.61, p = 0.014). Our data suggest that early-life sleep deprivation enhanced alcohol consumption in adolescent rats.

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“…On the other side, irritable bowel syndrome (IBS) is a functional digestive disorder characterized by stool abnormalities, diarrhea, constipation or both form alternating between them over time, accompanied by abdominal pain, bloating or flatulence, without anatomical/ structural deficits [15]. Even more importantly, this disorder is considered lately a biopsychological clinical entity which is highly influenced and could be exacerbated by stressful stimuli [16,17], being associated with a variety of neuropsychiatric manifestations [15,18,19].…”

Section: Abstract: Cortisol Serum Depression Irritable Bowel Syndromementioning

confidence: 99%

Serum Cortisol Levels Modifications in Patients with Depression and Irritable Bowel Syndrome

Hrițcu¹,

Pădurariu²,

Ciobîcă³

et al. 2019

Rev. Chim.

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The correlations between depression and stress are quite accepted nowadays, as well as the fact that cortisol levels could also represent fundamental risk factors for major depressive disorder. However, when it comes to the modifications of cortisol levels in depression, the results are controversial. Same goes for the cortisol modifications in irritable bowel syndrome (IBS), which is a functional digestive disorder characterized by a strong biopsychological component, being highly influenced or exacerbated by stressful stimuli. Thus, in the present report we were interested in seeing if there are any serum cortisol levels variations in patients with depression and irritable bowel syndrome, by looking at cortisol modifications in two different groups of patients: a control group with major depression disorder and another study group composed by major depression disorder + IBS patients. Thus, our data presented here are suggesting that there is a significant increase in cortisol levels in the morning serum of the patients with major depression disorder + IBS, as compared to the group having only depression. The results presented here could have an important relevance in the context of managing the stress-related factors in the pathological entities described above.

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A new biological approach in generating an irritable bowel syndrome rat model – focusing on depression in sucrose splash test and body weight change

Cited by 8 publications

References 0 publications

Antioxidant Capacity and Behavioral Relevance of a Polyphenolic Extract of Chrysanthellum americanum in a Rat Model of Irritable Bowel Syndrome

Antioxidant Capacity and Behavioral Relevance of a Polyphenolic Extract of Chrysanthellum americanum in a Rat Model of Irritable Bowel Syndrome

Early-Life Sleep Deprivation Enhanced Alcohol Consumption in Adolescent Rats

Serum Cortisol Levels Modifications in Patients with Depression and Irritable Bowel Syndrome

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